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The map label for this gene is trmB [H]

Identifier: 57238150

GI number: 57238150

Start: 1331083

End: 1332261

Strand: Reverse

Name: trmB [H]

Synonym: CJE1414

Alternate gene names: 57238150

Gene position: 1332261-1331083 (Counterclockwise)

Preceding gene: 57238151

Following gene: 57238149

Centisome position: 74.94

GC content: 27.82

Gene sequence:

>1179_bases
GTGCCAAATTTTAAAAGTAAAAAGATCAAAGAAATAAATCTGCCTTATAGTAAGGATGATGTTGAATTTCTTTGGCTTGC
TAAGAATGATAATGTTAGTCTTATTTATACAAAGGTTCAAGAAGAGAGTTTTTTTCTGCAGATTAAAAAAGCACAAAATG
GTTTTGTGATTAAAGGCGATAAACATACAAAACCTTCTAAAATAGGTTATTTGCAAAAGGCTTTAAAAATCTTTAAAGAA
GGTTTTTGTGAGGATATTATCAATGAAGCTTTTGGACTTAAAAACAATGCTTTAATTGAAAAAACACCATTTATCGTTGA
CAATTTTGATGAGCTTTTGTCTAGGCTTCAAGGTAAAATTTATATAGAAATAGGCTTTGGATCAGGAAGACATTTGCTTT
ATCAAGCAAAAGAAAATCCAAATGTTTTAATTTTAGGGGTGGAAATTTACAACCCAGCTTTAACTCAAGTGGCTAAACTT
GCTAAAGCACAAAATGTAAATAATATTTTGCTAATCCAAAGTGATGCGAGATTGTTATTGAGTGTATTAAAATCAAAATC
AGTTGAAAAGATTTTTCTACATTTTCCTGTTCCTTGGGACAAAAAGCCTCATAGGCGAGTTATTGGGAAAGATTTTTGCA
AAGAATGTGCTAGGGTTTTAACCCAAAATGGTCGTTTTGAACTTAGAACAGATAGTTTTGAGTATTTTAATTTTACTCTA
GAACAATTTTTGACTTTTCCTGTTCCAAAATTTAGTCTTAGAAAAAATGAAAATTTAGAAATTTCAAGCAAATATGAAGA
TAGATGGAAAAAGCAAGAAAAAAATATATATGATCTTTGGGTGTGGAATTTTAACCAAGAGTGTAAAAACTATGAGTTAA
ATGAATTTAATCTCTCATCGGTTGAATTTTCTAAAGAGGATTTGAAAAAAATAGAGCAGAACTTTAAAAATATTACTATA
AAAAAAGATGATTTTTTCTTGCATTTTGAAAGCATCTATAAACAAGATGAGAATTTGCTTTTAAAGGTTGCTTTTGGAGC
TTTTAATAAGCCTGAACATTGTTATTTGCATCTTGATAAAACGATTGATTTTGCTTTTAAAGAGCCATTTAAAATTCAAG
AAAATATTAAAGCGATAAATGAATTAAAAGAAATTTTAAAAGTTCAGTTTAAAATATAA

Upstream 100 bases:

>100_bases
TAAAAGCTTTACCAGGGGTGGAATATAGTTATGAAGTTATTGCTATTGATTCAGCTGGACTTCGTTCAGAACCTTCAAGC
AAAGTTAAGGCGGCTCAGTA

Downstream 100 bases:

>100_bases
TTTTTCTTGAAAATTAAGCAAAAATAAAGCATAATTTAAAAATTTAAAAAGGGAAATTAAGGAAAAAGGATGCCTAATTT
AATCCAAGCAAATAAACTTA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPNFKSKKIKEINLPYSKDDVEFLWLAKNDNVSLIYTKVQEESFFLQIKKAQNGFVIKGDKHTKPSKIGYLQKALKIFKE
GFCEDIINEAFGLKNNALIEKTPFIVDNFDELLSRLQGKIYIEIGFGSGRHLLYQAKENPNVLILGVEIYNPALTQVAKL
AKAQNVNNILLIQSDARLLLSVLKSKSVEKIFLHFPVPWDKKPHRRVIGKDFCKECARVLTQNGRFELRTDSFEYFNFTL
EQFLTFPVPKFSLRKNENLEISSKYEDRWKKQEKNIYDLWVWNFNQECKNYELNEFNLSSVEFSKEDLKKIEQNFKNITI
KKDDFFLHFESIYKQDENLLLKVAFGAFNKPEHCYLHLDKTIDFAFKEPFKIQENIKAINELKEILKVQFKI

Sequences:

>Translated_392_residues
MPNFKSKKIKEINLPYSKDDVEFLWLAKNDNVSLIYTKVQEESFFLQIKKAQNGFVIKGDKHTKPSKIGYLQKALKIFKE
GFCEDIINEAFGLKNNALIEKTPFIVDNFDELLSRLQGKIYIEIGFGSGRHLLYQAKENPNVLILGVEIYNPALTQVAKL
AKAQNVNNILLIQSDARLLLSVLKSKSVEKIFLHFPVPWDKKPHRRVIGKDFCKECARVLTQNGRFELRTDSFEYFNFTL
EQFLTFPVPKFSLRKNENLEISSKYEDRWKKQEKNIYDLWVWNFNQECKNYELNEFNLSSVEFSKEDLKKIEQNFKNITI
KKDDFFLHFESIYKQDENLLLKVAFGAFNKPEHCYLHLDKTIDFAFKEPFKIQENIKAINELKEILKVQFKI
>Mature_391_residues
PNFKSKKIKEINLPYSKDDVEFLWLAKNDNVSLIYTKVQEESFFLQIKKAQNGFVIKGDKHTKPSKIGYLQKALKIFKEG
FCEDIINEAFGLKNNALIEKTPFIVDNFDELLSRLQGKIYIEIGFGSGRHLLYQAKENPNVLILGVEIYNPALTQVAKLA
KAQNVNNILLIQSDARLLLSVLKSKSVEKIFLHFPVPWDKKPHRRVIGKDFCKECARVLTQNGRFELRTDSFEYFNFTLE
QFLTFPVPKFSLRKNENLEISSKYEDRWKKQEKNIYDLWVWNFNQECKNYELNEFNLSSVEFSKEDLKKIEQNFKNITIK
KDDFFLHFESIYKQDENLLLKVAFGAFNKPEHCYLHLDKTIDFAFKEPFKIQENIKAINELKEILKVQFKI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=186, Percent_Identity=26.3440860215054, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 46215; Mature: 46084

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNFKSKKIKEINLPYSKDDVEFLWLAKNDNVSLIYTKVQEESFFLQIKKAQNGFVIKGD
CCCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEEEEECCCEEEEEEEECCCCEEEECC
KHTKPSKIGYLQKALKIFKEGFCEDIINEAFGLKNNALIEKTPFIVDNFDELLSRLQGKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCHHHHHHHCCCEE
YIEIGFGSGRHLLYQAKENPNVLILGVEIYNPALTQVAKLAKAQNVNNILLIQSDARLLL
EEEEECCCCCEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
SVLKSKSVEKIFLHFPVPWDKKPHRRVIGKDFCKECARVLTQNGRFELRTDSFEYFNFTL
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
EQFLTFPVPKFSLRKNENLEISSKYEDRWKKQEKNIYDLWVWNFNQECKNYELNEFNLSS
HHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCC
VEFSKEDLKKIEQNFKNITIKKDDFFLHFESIYKQDENLLLKVAFGAFNKPEHCYLHLDK
CCCCHHHHHHHHHHHHEEEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
TIDFAFKEPFKIQENIKAINELKEILKVQFKI
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNFKSKKIKEINLPYSKDDVEFLWLAKNDNVSLIYTKVQEESFFLQIKKAQNGFVIKGD
CCCCCCCEEEECCCCCCCCEEEEEEEECCCEEEEEEEECCCEEEEEEEECCCCEEEECC
KHTKPSKIGYLQKALKIFKEGFCEDIINEAFGLKNNALIEKTPFIVDNFDELLSRLQGKI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEECCHHHHHHHCCCEE
YIEIGFGSGRHLLYQAKENPNVLILGVEIYNPALTQVAKLAKAQNVNNILLIQSDARLLL
EEEEECCCCCEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCHHHHH
SVLKSKSVEKIFLHFPVPWDKKPHRRVIGKDFCKECARVLTQNGRFELRTDSFEYFNFTL
HHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHH
EQFLTFPVPKFSLRKNENLEISSKYEDRWKKQEKNIYDLWVWNFNQECKNYELNEFNLSS
HHHHHCCCCCCCCCCCCCCEECCHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCC
VEFSKEDLKKIEQNFKNITIKKDDFFLHFESIYKQDENLLLKVAFGAFNKPEHCYLHLDK
CCCCHHHHHHHHHHHHEEEEECCCEEEEEHHHHCCCCCEEEEEEECCCCCCCEEEEEEEC
TIDFAFKEPFKIQENIKAINELKEILKVQFKI
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA