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The map label for this gene is epsH [H]

Identifier: 57238017

GI number: 57238017

Start: 1195965

End: 1196852

Strand: Reverse

Name: epsH [H]

Synonym: CJE1278

Alternate gene names: 57238017

Gene position: 1196852-1195965 (Counterclockwise)

Preceding gene: 57238018

Following gene: 57238013

Centisome position: 67.32

GC content: 21.28

Gene sequence:

>888_bases
ATGCCTAAAATTTCAATCATCTTGCCAACTTTTAATGTAGAAAAATATATAGCCAAAGCTTTAGAAAGTTGTATCAATCA
AAGTTTTAAAGATATAGAAATAATTGTAGTGGATGATTGTGGGAGTGATAAAAGTATAGATATAGCTAAAGAATATGCCA
AAAAAGATAAAAGGATAAAAATAGTTCACAATGAAAAAAATTTGGGTACTTTTGCAGCAAGAAATAATGGTGTTAAACAT
TCAAATAGTGAGTATTTGATGTTTTTAGATCCTGATGATTTTTTAGAGCTTGAGGCTTGTGAAAAATTAATACTTTTAAT
AAATACTTATAAAATATGCCAATTTTCTTTTACTCAGATTAATAATGGAATTAAATTAAAATCAGTATTTAAAGATACAC
TTAAAAATTTAAAAGAATTTAAAGGAATTTATTGGAATATCTGTACTCTGTTTGTAAAAAAGGATTTTTATTTAAAATCC
CTAGAAGAACTTTTTTTGATAGATAAAAAACTCCTTGTAGCCGAAGATATGCTTGCGGTTTTTTTCTTGATAAACAATTT
AAAATATGATGAATACTTGCAAGTTGATTTGCATTTGTATAATTATGTAGATCATTCTTTTTCGATTACTAAGCGACGAA
AAAATAAAGAGAAATTTCAGGAATGTTTGGATGATTATTTTTATATTTTAAAATATATAAAAGAAAATAAAATTCTTAAA
GAAAATTTAAAGTCATATGTTTTGATTTATTTGCAAATTGATATTTTAAAATTTTTATTTAATAATAAAAAAATAAATTA
TTTTAAATATAAATTATTAAAAAAAATTTATAAATGTAAAATGCTATTTTTAAAACTTAATTTCTCCAAGAAATTTTTTT
ATTTTTGA

Upstream 100 bases:

>100_bases
GATTTATTTACTTTAGTGGGTATCAATTTAAAACTTTTTAGATTTAAATTTATGTTAAGATTTTATTTTCGCCACCCAAA
AAAGATTTTTAAGAAAAACA

Downstream 100 bases:

>100_bases
TGGTATCTAAATAAATTTGATGATTGCTTTCGAGCATTTTTTTGCTATTTTCTTTGTGATAAATATGATAAGCAATAGCT
TTAAATTTTAAGCGTCTAAA

Product: lipooligosaccharide biosynthesis galactosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MPKISIILPTFNVEKYIAKALESCINQSFKDIEIIVVDDCGSDKSIDIAKEYAKKDKRIKIVHNEKNLGTFAARNNGVKH
SNSEYLMFLDPDDFLELEACEKLILLINTYKICQFSFTQINNGIKLKSVFKDTLKNLKEFKGIYWNICTLFVKKDFYLKS
LEELFLIDKKLLVAEDMLAVFFLINNLKYDEYLQVDLHLYNYVDHSFSITKRRKNKEKFQECLDDYFYILKYIKENKILK
ENLKSYVLIYLQIDILKFLFNNKKINYFKYKLLKKIYKCKMLFLKLNFSKKFFYF

Sequences:

>Translated_295_residues
MPKISIILPTFNVEKYIAKALESCINQSFKDIEIIVVDDCGSDKSIDIAKEYAKKDKRIKIVHNEKNLGTFAARNNGVKH
SNSEYLMFLDPDDFLELEACEKLILLINTYKICQFSFTQINNGIKLKSVFKDTLKNLKEFKGIYWNICTLFVKKDFYLKS
LEELFLIDKKLLVAEDMLAVFFLINNLKYDEYLQVDLHLYNYVDHSFSITKRRKNKEKFQECLDDYFYILKYIKENKILK
ENLKSYVLIYLQIDILKFLFNNKKINYFKYKLLKKIYKCKMLFLKLNFSKKFFYF
>Mature_294_residues
PKISIILPTFNVEKYIAKALESCINQSFKDIEIIVVDDCGSDKSIDIAKEYAKKDKRIKIVHNEKNLGTFAARNNGVKHS
NSEYLMFLDPDDFLELEACEKLILLINTYKICQFSFTQINNGIKLKSVFKDTLKNLKEFKGIYWNICTLFVKKDFYLKSL
EELFLIDKKLLVAEDMLAVFFLINNLKYDEYLQVDLHLYNYVDHSFSITKRRKNKEKFQECLDDYFYILKYIKENKILKE
NLKSYVLIYLQIDILKFLFNNKKINYFKYKLLKKIYKCKMLFLKLNFSKKFFYF

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles. Required for biofilm maintenance [H]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790044, Length=147, Percent_Identity=27.891156462585, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787259, Length=103, Percent_Identity=32.0388349514563, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788372, Length=94, Percent_Identity=36.1702127659575, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 35359; Mature: 35228

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKISIILPTFNVEKYIAKALESCINQSFKDIEIIVVDDCGSDKSIDIAKEYAKKDKRIK
CCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEE
IVHNEKNLGTFAARNNGVKHSNSEYLMFLDPDDFLELEACEKLILLINTYKICQFSFTQI
EEECCCCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEHHHH
NNGIKLKSVFKDTLKNLKEFKGIYWNICTLFVKKDFYLKSLEELFLIDKKLLVAEDMLAV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLINNLKYDEYLQVDLHLYNYVDHSFSITKRRKNKEKFQECLDDYFYILKYIKENKILK
HHHHHCCCCCCEEEEEEEEEEHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHH
ENLKSYVLIYLQIDILKFLFNNKKINYFKYKLLKKIYKCKMLFLKLNFSKKFFYF
HHHHHHEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
>Mature Secondary Structure 
PKISIILPTFNVEKYIAKALESCINQSFKDIEIIVVDDCGSDKSIDIAKEYAKKDKRIK
CCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEE
IVHNEKNLGTFAARNNGVKHSNSEYLMFLDPDDFLELEACEKLILLINTYKICQFSFTQI
EEECCCCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEHHHH
NNGIKLKSVFKDTLKNLKEFKGIYWNICTLFVKKDFYLKSLEELFLIDKKLLVAEDMLAV
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLINNLKYDEYLQVDLHLYNYVDHSFSITKRRKNKEKFQECLDDYFYILKYIKENKILK
HHHHHCCCCCCEEEEEEEEEEHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHHH
ENLKSYVLIYLQIDILKFLFNNKKINYFKYKLLKKIYKCKMLFLKLNFSKKFFYF
HHHHHHEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]