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The map label for this gene is metK [H]

Identifier: 57237978

GI number: 57237978

Start: 1155965

End: 1157176

Strand: Reverse

Name: metK [H]

Synonym: CJE1239

Alternate gene names: 57237978

Gene position: 1157176-1155965 (Counterclockwise)

Preceding gene: 57237992

Following gene: 57237976

Centisome position: 65.09

GC content: 35.56

Gene sequence:

>1212_bases
ATGAAAGGACTTTTTATGTATCTATTCACTTCAGAAGTCGTAAGTGCAGGTCATCCAGACAAGTGCGCTGATATAATCGC
TGATACGATAGTGGATATACTCTTGAAAAATGACAAAAATTCAAGGGTGGCGAGTGAGGTTTTTGTCGCAGGAAATAAGG
TTGTGATAGGAGGCGAAGTTAAGTCAAATCACAAACTTAGTAAAGCTGATTACGATAATTTAGTTAAAGATGTTTTAAAA
AACATTGGTTATGACGGAGCAGGCCATTTTAGCAAAGAGCAGTGTTTACATCCTGATGAGGTTGATGTTATGGTGTTTTT
AAATGAACAAAGTCCTGATATCAACCAAGGTGTCGATCAAGAAGATGGCGAAACAGGAGCAGGTGATCAAGGCATTATGT
TTGGTTTTGCAAGTTGCGAAGCTGAGGAATATATGCCAGCAGCTATTAGTTATGCAAGAATGCTATGTGATAGAGTTTAT
GCTTATGCAAAAGCAAATCCACATGAACTTGGAGTGGATATTAAAACTCAAGTGACTATAGATTATGGCACTAAGGCGAA
TTTTGAAAATTGCAAACCGCAAAGTATTCATACTATAGTAGTTTCTGCACCTTGTGTTGAAAGTATGAAAATAGAAGATT
TAAGATCTTTAGTGATGAAACTTATCTTAGATAGTAATTTACCAAAAGAACTTTTTGATCCTAATAAGACTAGAATTTTA
ATCAATCCAACTGGTAAATATGTTAATCATTCTTCTTTACATGATAGTGGTTTAACAGGAAGAAAACTTATAGTAGATAG
TTTTGGAGGTTATTCTCCAATAGGTGGTGGTGCGCAATCGAGCAAAGATTATACTAAAGTTGATAGAAGTGGACTTTATG
CAGGTAGATGGTTAGCTAAAAACATAGTTGCAGCAGGGCTTGCTAAAAAATGTATAGTTCAACTTAGTTATGCTATAGGT
GTTGCAAAACCTACTTCGGTAAGCGTGGATTGTATGGGCACAAATACCAGTGTAAATGACGATGTTTTAAGTGATTTTGT
AATGCAAAATTTCTCTTTAACGCCAAATTGGATCCGTGATAAATTTCATCTGGATAAACCAAGTAAAGAAACTTTTCTTT
ATGCGGATGTAGCAGCTCGTGGGCAAGTAGGACAAAAGGACTATCCTTGGGAAAAACTTGATGCTCTAGAACAGTTTAAA
AAGCTACTTTAA

Upstream 100 bases:

>100_bases
ATCAAAAGTTGATTTTAACTAACTTTTGCTTAATTTTAGAGAATTTTTAATTTATCAAGCTTTGATAAGTTTATTTGGAT
ACAATTGTGGTTCATTTTAA

Downstream 100 bases:

>100_bases
AAAATTAACAATATTATACAAGCTTAAACAAGGCTTGTATAATTATAAAGAATTAATAAAGTTATATTAATTTTTGACTT
AGAGGGATATTTTTCAATAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKGLFMYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKADYDNLVKDVLK
NIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGETGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVY
AYAKANPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMKIEDLRSLVMKLILDSNLPKELFDPNKTRIL
INPTGKYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIG
VAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKETFLYADVAARGQVGQKDYPWEKLDALEQFK
KLL

Sequences:

>Translated_403_residues
MKGLFMYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKADYDNLVKDVLK
NIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGETGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVY
AYAKANPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMKIEDLRSLVMKLILDSNLPKELFDPNKTRIL
INPTGKYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIG
VAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKETFLYADVAARGQVGQKDYPWEKLDALEQFK
KLL
>Mature_403_residues
MKGLFMYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEVKSNHKLSKADYDNLVKDVLK
NIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQEDGETGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVY
AYAKANPHELGVDIKTQVTIDYGTKANFENCKPQSIHTIVVSAPCVESMKIEDLRSLVMKLILDSNLPKELFDPNKTRIL
INPTGKYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAKNIVAAGLAKKCIVQLSYAIG
VAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRDKFHLDKPSKETFLYADVAARGQVGQKDYPWEKLDALEQFK
KLL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=394, Percent_Identity=40.8629441624365, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=38.8174807197943, Blast_Score=242, Evalue=4e-64,
Organism=Escherichia coli, GI1789311, Length=396, Percent_Identity=40.6565656565657, Blast_Score=274, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17538498, Length=390, Percent_Identity=40.5128205128205, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17538494, Length=401, Percent_Identity=40.1496259351621, Blast_Score=261, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=40.75, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17551082, Length=401, Percent_Identity=40.1496259351621, Blast_Score=251, Evalue=6e-67,
Organism=Caenorhabditis elegans, GI32565911, Length=343, Percent_Identity=39.9416909620991, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI32565905, Length=400, Percent_Identity=37, Blast_Score=221, Evalue=5e-58,
Organism=Caenorhabditis elegans, GI25145635, Length=353, Percent_Identity=40.2266288951841, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32565903, Length=319, Percent_Identity=39.8119122257053, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=41.4634146341463, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=402, Percent_Identity=41.7910447761194, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6320710, Length=396, Percent_Identity=40.9090909090909, Blast_Score=257, Evalue=2e-69,
Organism=Drosophila melanogaster, GI45552159, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580529, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580537, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580533, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580535, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580531, Length=395, Percent_Identity=40.253164556962, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI24580527, Length=394, Percent_Identity=39.8477157360406, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24580525, Length=394, Percent_Identity=39.8477157360406, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24580541, Length=381, Percent_Identity=38.8451443569554, Blast_Score=230, Evalue=1e-60,
Organism=Drosophila melanogaster, GI24580539, Length=135, Percent_Identity=36.2962962962963, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44232; Mature: 44232

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00376 ADOMET_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGLFMYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEV
CCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCC
KSNHKLSKADYDNLVKDVLKNIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCC
EDGETGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKANPHELGVDIKTQVTI
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEEE
DYGTKANFENCKPQSIHTIVVSAPCVESMKIEDLRSLVMKLILDSNLPKELFDPNKTRIL
ECCCCCCCCCCCCCCEEEEEEECCCCHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEE
INPTGKYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAK
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHH
NIVAAGLAKKCIVQLSYAIGVAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHH
KFHLDKPSKETFLYADVAARGQVGQKDYPWEKLDALEQFKKLL
HHCCCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MKGLFMYLFTSEVVSAGHPDKCADIIADTIVDILLKNDKNSRVASEVFVAGNKVVIGGEV
CCCEEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHEECCCEEEECCCC
KSNHKLSKADYDNLVKDVLKNIGYDGAGHFSKEQCLHPDEVDVMVFLNEQSPDINQGVDQ
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCC
EDGETGAGDQGIMFGFASCEAEEYMPAAISYARMLCDRVYAYAKANPHELGVDIKTQVTI
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEEEEEE
DYGTKANFENCKPQSIHTIVVSAPCVESMKIEDLRSLVMKLILDSNLPKELFDPNKTRIL
ECCCCCCCCCCCCCCEEEEEEECCCCHHCCHHHHHHHHHHHHHCCCCCHHHCCCCCCEEE
INPTGKYVNHSSLHDSGLTGRKLIVDSFGGYSPIGGGAQSSKDYTKVDRSGLYAGRWLAK
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHH
NIVAAGLAKKCIVQLSYAIGVAKPTSVSVDCMGTNTSVNDDVLSDFVMQNFSLTPNWIRD
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHH
KFHLDKPSKETFLYADVAARGQVGQKDYPWEKLDALEQFKKLL
HHCCCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA