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The map label for this gene is ssb [H]

Identifier: 57237953

GI number: 57237953

Start: 1131891

End: 1132439

Strand: Direct

Name: ssb [H]

Synonym: CJE1214

Alternate gene names: 57237953

Gene position: 1131891-1132439 (Clockwise)

Preceding gene: 57237952

Following gene: 57237954

Centisome position: 63.67

GC content: 34.24

Gene sequence:

>549_bases
ATGTTTAACAAAGTTGTTTTGGTTGGAAATCTTACACGTGATATAGAAATGCGTTATGCACAAAGCGGTAGTGCTATTGG
AGCTTCCGCTATAGCAGTAACAAGAAGATTTACCGCAAATGGAGAAAAAAGAGAAGAAACTTGCTTTATTGATATAAGCT
TTTATGGTCGCACCGCAGAAGTTGCAAACCAATACCTTACAAAAGGTTCAAAAGTACTCATAGAAGGACGTTTGAGATTT
GAACAATGGAGTGATCAAAATGGACAAAATCGCTCTAAACATAGCATTCAAGTTGAAAATATGGAAATGCTTGGCAATTC
CAATGCTCCACAACAAGGCGGTTTTGGCAATAATAGCTTTTCAAACAACAATTATAGTGGAAACTATGAAAATCAAAGCT
ATGATCCTTATATGAGTGAAAATCAAAATTTTAATAAAGCAAAAGCAAACCCTGCTCCACAAAGAAACCAAAACCCACAA
CACGAAGAAAAATTAAAAGAAATTGATATCGATGCTTATGATAGCGATGATACTAATTTACCATTTTAA

Upstream 100 bases:

>100_bases
AAAAAGAAATTGCAGCTTGGGAAAAACTAAGCCACGGGATTAAGCAATCTAAAAAAGAAATCAAACCTTTAGATGCTCCT
GAAATTCAATAAGGAAAAAG

Downstream 100 bases:

>100_bases
AGGATAAACTATGGCAGAAAAAAGAAAATACTCTCGCAAATATTGCAAATACACTGAAGCAAAAGTTGAATTTATTGATT
ATAAAGATACAGCAATGTTA

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MFNKVVLVGNLTRDIEMRYAQSGSAIGASAIAVTRRFTANGEKREETCFIDISFYGRTAEVANQYLTKGSKVLIEGRLRF
EQWSDQNGQNRSKHSIQVENMEMLGNSNAPQQGGFGNNSFSNNNYSGNYENQSYDPYMSENQNFNKAKANPAPQRNQNPQ
HEEKLKEIDIDAYDSDDTNLPF

Sequences:

>Translated_182_residues
MFNKVVLVGNLTRDIEMRYAQSGSAIGASAIAVTRRFTANGEKREETCFIDISFYGRTAEVANQYLTKGSKVLIEGRLRF
EQWSDQNGQNRSKHSIQVENMEMLGNSNAPQQGGFGNNSFSNNNYSGNYENQSYDPYMSENQNFNKAKANPAPQRNQNPQ
HEEKLKEIDIDAYDSDDTNLPF
>Mature_182_residues
MFNKVVLVGNLTRDIEMRYAQSGSAIGASAIAVTRRFTANGEKREETCFIDISFYGRTAEVANQYLTKGSKVLIEGRLRF
EQWSDQNGQNRSKHSIQVENMEMLGNSNAPQQGGFGNNSFSNNNYSGNYENQSYDPYMSENQNFNKAKANPAPQRNQNPQ
HEEKLKEIDIDAYDSDDTNLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Escherichia coli, GI1790494, Length=118, Percent_Identity=43.2203389830509, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 20597; Mature: 20597

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKVVLVGNLTRDIEMRYAQSGSAIGASAIAVTRRFTANGEKREETCFIDISFYGRTAE
CCCCEEEEECCCCHHHHEECCCCCCCCHHHHHEEEEECCCCCCCCCEEEEEEEECCCHHH
VANQYLTKGSKVLIEGRLRFEQWSDQNGQNRSKHSIQVENMEMLGNSNAPQQGGFGNNSF
HHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCC
SNNNYSGNYENQSYDPYMSENQNFNKAKANPAPQRNQNPQHEEKLKEIDIDAYDSDDTNL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
PF
CC
>Mature Secondary Structure
MFNKVVLVGNLTRDIEMRYAQSGSAIGASAIAVTRRFTANGEKREETCFIDISFYGRTAE
CCCCEEEEECCCCHHHHEECCCCCCCCHHHHHEEEEECCCCCCCCCEEEEEEEECCCHHH
VANQYLTKGSKVLIEGRLRFEQWSDQNGQNRSKHSIQVENMEMLGNSNAPQQGGFGNNSF
HHHHHHHCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCC
SNNNYSGNYENQSYDPYMSENQNFNKAKANPAPQRNQNPQHEEKLKEIDIDAYDSDDTNL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCC
PF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9742705; 10688204 [H]