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The map label for this gene is tgt [H]

Identifier: 57237835

GI number: 57237835

Start: 1019079

End: 1020200

Strand: Direct

Name: tgt [H]

Synonym: CJE1090

Alternate gene names: 57237835

Gene position: 1019079-1020200 (Clockwise)

Preceding gene: 57237829

Following gene: 57237836

Centisome position: 57.32

GC content: 32.89

Gene sequence:

>1122_bases
ATGGAATTTAAATTAAAGCATAAAGATGGAATGGCAAGGGTTTGTGAAATCACTACCGCACACAGCACTTTTTTAACCCC
TGTTTTTATGCCTGTTGGCACAGTAGGAGCGGTTAAAAGTCTTGATGCTAATGATATGAAAAATGAGCTTGATGCAAAAA
TTATTTTAGCAAATACCTATCACATGTACTTGCGTCCAACTTCCAAAGTAGTAAAAGATTTTGGAGGTTTACATGGATTT
ACGAAATTTGATAGAAGTTTTTTAACCGATAGTGGTGGTTTTCAAGCTTTTTCTTTAAGTAAAAATTCCAAACATTTTAA
TGAAGGCATAGAATTTAAAAGTCACATTGATGGAAGTCGTCATCTTTTTACTCCAAAAAGTGTTTTAGATGCTCAATATG
ATTTTAATTCTGATATTATGATGATTTTAGATGATTTAGTAGCCCTACCCGCAACCAAAGAAAGAGTTAAAATTTCTGTT
GATCGCACTATACTTTGGGCAAAAGAGGCTATTACTTATCATAAAAATATGCAAAATAAAGGTATAGGTATAGGGCAAAA
TATTTTTGGTATTATCCAAGGTGGTACAGATTATGAAGAAAGAAAGCGTTGCGCTTTAAGTTTAAATGAAATGCCATTTG
ATGGTTTAGCTATAGGTGGGCTTAGCGTGGGCGAAGAAAACGCCTTGATGTACGAAACAGTCCAAAACTTAAACCCTTAT
CTAGATGAAAATCGTCCAAGATATTTAATGGGCGTAGGAACGCCTGAAGATTTAGTAGAAAATGTTGAGCGAGGAGTAGA
TATGTTTGACTGTGTGATGCCAACTAGAAATGCAAGAAATGGAACATTTTTCACAAGTTTTGGAAAATTTAATATCAAAA
AAGCAGAATTTATCAACGATCATGAAGTTATAGATCCTACTTGCTCATGTTATACTTGTCGTAATTTTTCAAGGGGTTAT
TTAAACCATCTTTTTAAAGCTAAAGAACTTACTTTTTTTCGTTTGGCAAGTCTGCACAATCTACATTATTATCTTGAACT
TGCAAGAAAGATGCGTGAAGCAATTTTGAACAATTCTTTTACGCAATTTAAAAGAAATTTTTATCACTTAAGAGGCAAAT
AA

Upstream 100 bases:

>100_bases
TTTTCAAAACAAAGCCTTTCTAAGAAATGCTTTGCTAAAATACCATCTATTATAATTAAAATATTATTCATTTTAAACTC
CAAAAAGGAAATTATAACAA

Downstream 100 bases:

>100_bases
AATGCTAGAGCTACACGAAAATCTAAAAAAAATCCTGCAAGCAAAGAATTTAGAAACTTTCTATAGCGAAATTTATGGAC
AAAAAATTTTTGTTTACGTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTYHMYLRPTSKVVKDFGGLHGF
TKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSRHLFTPKSVLDAQYDFNSDIMMILDDLVALPATKERVKISV
DRTILWAKEAITYHKNMQNKGIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQNLNPY
LDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEFINDHEVIDPTCSCYTCRNFSRGY
LNHLFKAKELTFFRLASLHNLHYYLELARKMREAILNNSFTQFKRNFYHLRGK

Sequences:

>Translated_373_residues
MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTYHMYLRPTSKVVKDFGGLHGF
TKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSRHLFTPKSVLDAQYDFNSDIMMILDDLVALPATKERVKISV
DRTILWAKEAITYHKNMQNKGIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQNLNPY
LDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEFINDHEVIDPTCSCYTCRNFSRGY
LNHLFKAKELTFFRLASLHNLHYYLELARKMREAILNNSFTQFKRNFYHLRGK
>Mature_373_residues
MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTYHMYLRPTSKVVKDFGGLHGF
TKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSRHLFTPKSVLDAQYDFNSDIMMILDDLVALPATKERVKISV
DRTILWAKEAITYHKNMQNKGIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQNLNPY
LDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEFINDHEVIDPTCSCYTCRNFSRGY
LNHLFKAKELTFFRLASLHNLHYYLELARKMREAILNNSFTQFKRNFYHLRGK

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=356, Percent_Identity=39.0449438202247, Blast_Score=264, Evalue=1e-70,
Organism=Escherichia coli, GI1786607, Length=373, Percent_Identity=42.0911528150134, Blast_Score=313, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17544680, Length=370, Percent_Identity=38.3783783783784, Blast_Score=243, Evalue=9e-65,
Organism=Caenorhabditis elegans, GI25146502, Length=198, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=42.6966292134831, Blast_Score=282, Evalue=3e-76,
Organism=Drosophila melanogaster, GI21355311, Length=243, Percent_Identity=26.7489711934156, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42554; Mature: 42554

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTY
CCEEECCCCCCEEHHEEECCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCEEEEEEEE
HMYLRPTSKVVKDFGGLHGFTKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSR
EEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCHHHCCCCCC
HLFTPKSVLDAQYDFNSDIMMILDDLVALPATKERVKISVDRTILWAKEAITYHKNMQNK
EEECCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHHHHCCCCC
GIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQNLNPY
CCCCCCCEEEEEECCCCHHHHHHHHEECCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCC
LDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEFIND
CCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCCCCHHHHCCC
HEVIDPTCSCYTCRNFSRGYLNHLFKAKELTFFRLASLHNLHYYLELARKMREAILNNSF
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TQFKRNFYHLRGK
HHHHHHHHEECCC
>Mature Secondary Structure
MEFKLKHKDGMARVCEITTAHSTFLTPVFMPVGTVGAVKSLDANDMKNELDAKIILANTY
CCEEECCCCCCEEHHEEECCCHHHHHHHHHCCCCHHHHHCCCCHHHHHHCCCEEEEEEEE
HMYLRPTSKVVKDFGGLHGFTKFDRSFLTDSGGFQAFSLSKNSKHFNEGIEFKSHIDGSR
EEEECCHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHCCCCHHHCCCCCC
HLFTPKSVLDAQYDFNSDIMMILDDLVALPATKERVKISVDRTILWAKEAITYHKNMQNK
EEECCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHHHHCCCCC
GIGIGQNIFGIIQGGTDYEERKRCALSLNEMPFDGLAIGGLSVGEENALMYETVQNLNPY
CCCCCCCEEEEEECCCCHHHHHHHHEECCCCCCCCEEECCCCCCCCCHHHHHHHHHCCCC
LDENRPRYLMGVGTPEDLVENVERGVDMFDCVMPTRNARNGTFFTSFGKFNIKKAEFIND
CCCCCCEEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCCCCCCHHHHCCC
HEVIDPTCSCYTCRNFSRGYLNHLFKAKELTFFRLASLHNLHYYLELARKMREAILNNSF
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TQFKRNFYHLRGK
HHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA