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The map label for this gene is glcF [H]

Identifier: 57237816

GI number: 57237816

Start: 999643

End: 1000908

Strand: Reverse

Name: glcF [H]

Synonym: CJE1071

Alternate gene names: 57237816

Gene position: 1000908-999643 (Counterclockwise)

Preceding gene: 57237817

Following gene: 57237815

Centisome position: 56.3

GC content: 30.73

Gene sequence:

>1266_bases
ATGAAATTTAGTCAAATAAGTGATGCTTGTGTTAAATGTGGCAAATGTATACCTGTTTGTACTATACACGAAGAAAATCG
CGATGAAATTACTAGTCCAAGAGGATTTTTAGATCTTTTAGCTGCTTATAAAGAGGGAAGTCTAGAACTTGATAAAGAAG
CTAAGAAAGTTTTTGAATCTTGTTTTTTATGCACCAATTGTGTTGAAGTATGTCCAAGTAAATTAAGAGTAGATAATGCC
ATAGAAGAAGTTCGTTATGATATAGCTAAAAAATTTGGTATAGCTTGGTATAAAAAACTTATTTTTTTCTTTTTAAGAAG
ACGCAAAATTTTAGATTTGGTAGCTAAGCTTGGCTATGTGTTTCAAAGTTGTGCGTTTAAAATTCAAAATGAGAATGAAA
ATACAGGTATGAAGGCAAGATTTTCCATGCCTTTTGTTAAAAAGGGAAGATTGCTTACAAGTTTTAATAAAAAAAGTTTC
TTAAATTCTAATCCAAAATTTATCAATAATGATGGAGAAAAAACAATAGGTTTTTTTGTAGGTTGTTTGGCGAATTATTT
TTACATAGATACGGCTAATGCAGTGCTTAAAATCGCTAAGGAATTAAAAATCAATGTGGATTTGATGAAAGAACAAGTGT
GTTGTGGAGCGCCGCAATTTTTTACGGGTGATTTTAAAAGTGTTGAAGTTTTGGCTAAAAAAAATATTGAGTATTTTGAA
AAAAAATTAGAAAAATTAGATGCTATTATAGTTCCTGAGGCCACTTGTTCGGCTATGTTAAAGATAGATTATGAACATTT
TTTTATAATGCAAAATGATTTAGACTGGGCAAAACGCGCAAAATGTGTGTCATCTAAGATATATATGGCTAGCGAGTATT
TTTATAAATTTACTTCTTTAGAAGAAATTTTAAAAACAAAAAATAAAATTAATTATAGCATTACTTATCACGATCCTTGC
CATGCAAGAAAAATGCAAGGGGTTTTTAAAGAGCCAAGAGAACTTTTAAAAACAAATTATCATTTTGTAGAAATGAGTAA
TTCTAATACTTGTTGCGGTTTTGGCGGGGTGGGTATGCAAACGGATTATTATGATAGAGCCTTAAGTGTAGGGCTTAAAA
AAGCACAAATGATCGATGAAAGTAAGGCTAGTGTGGTAAGTGCCGAGTGTTCAGCTTGCAGAATGCAAATTTCAAATGCA
TTAGAACAAAATTCAAGCAAGGTTGTTTTTGCTAGTCCTTTAGAACTTATAGCAAAAGCTTTGTGA

Upstream 100 bases:

>100_bases
GAAACAGGCGTTTTGCTTATAAGAAATATTGCCATGTGTTTTGATGCTTATATGAAAAACATTAGTGAAGATAAAAAAGT
ATTCTCAAAAACGGTGTAAG

Downstream 100 bases:

>100_bases
TGATGAAGATAGTATTTAAAGGCAAAAGTTCTGAATATGAAATTCAAAGATCGTGCTTTTGTGTTGATGCTTTTGTGATT
AAAGATAAGATTGAAGAACG

Product: iron-sulfur cluster-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKFSQISDACVKCGKCIPVCTIHEENRDEITSPRGFLDLLAAYKEGSLELDKEAKKVFESCFLCTNCVEVCPSKLRVDNA
IEEVRYDIAKKFGIAWYKKLIFFFLRRRKILDLVAKLGYVFQSCAFKIQNENENTGMKARFSMPFVKKGRLLTSFNKKSF
LNSNPKFINNDGEKTIGFFVGCLANYFYIDTANAVLKIAKELKINVDLMKEQVCCGAPQFFTGDFKSVEVLAKKNIEYFE
KKLEKLDAIIVPEATCSAMLKIDYEHFFIMQNDLDWAKRAKCVSSKIYMASEYFYKFTSLEEILKTKNKINYSITYHDPC
HARKMQGVFKEPRELLKTNYHFVEMSNSNTCCGFGGVGMQTDYYDRALSVGLKKAQMIDESKASVVSAECSACRMQISNA
LEQNSSKVVFASPLELIAKAL

Sequences:

>Translated_421_residues
MKFSQISDACVKCGKCIPVCTIHEENRDEITSPRGFLDLLAAYKEGSLELDKEAKKVFESCFLCTNCVEVCPSKLRVDNA
IEEVRYDIAKKFGIAWYKKLIFFFLRRRKILDLVAKLGYVFQSCAFKIQNENENTGMKARFSMPFVKKGRLLTSFNKKSF
LNSNPKFINNDGEKTIGFFVGCLANYFYIDTANAVLKIAKELKINVDLMKEQVCCGAPQFFTGDFKSVEVLAKKNIEYFE
KKLEKLDAIIVPEATCSAMLKIDYEHFFIMQNDLDWAKRAKCVSSKIYMASEYFYKFTSLEEILKTKNKINYSITYHDPC
HARKMQGVFKEPRELLKTNYHFVEMSNSNTCCGFGGVGMQTDYYDRALSVGLKKAQMIDESKASVVSAECSACRMQISNA
LEQNSSKVVFASPLELIAKAL
>Mature_421_residues
MKFSQISDACVKCGKCIPVCTIHEENRDEITSPRGFLDLLAAYKEGSLELDKEAKKVFESCFLCTNCVEVCPSKLRVDNA
IEEVRYDIAKKFGIAWYKKLIFFFLRRRKILDLVAKLGYVFQSCAFKIQNENENTGMKARFSMPFVKKGRLLTSFNKKSF
LNSNPKFINNDGEKTIGFFVGCLANYFYIDTANAVLKIAKELKINVDLMKEQVCCGAPQFFTGDFKSVEVLAKKNIEYFE
KKLEKLDAIIVPEATCSAMLKIDYEHFFIMQNDLDWAKRAKCVSSKIYMASEYFYKFTSLEEILKTKNKINYSITYHDPC
HARKMQGVFKEPRELLKTNYHFVEMSNSNTCCGFGGVGMQTDYYDRALSVGLKKAQMIDESKASVVSAECSACRMQISNA
LEQNSSKVVFASPLELIAKAL

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1788576, Length=424, Percent_Identity=22.877358490566, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1786497, Length=235, Percent_Identity=24.2553191489362, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 47993; Mature: 47993

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSQISDACVKCGKCIPVCTIHEENRDEITSPRGFLDLLAAYKEGSLELDKEAKKVFES
CCCHHHHHHHHHCCCCCEEEEECCCCCHHCCCCHHHHHHHHHHCCCCCEECHHHHHHHHH
CFLCTNCVEVCPSKLRVDNAIEEVRYDIAKKFGIAWYKKLIFFFLRRRKILDLVAKLGYV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSCAFKIQNENENTGMKARFSMPFVKKGRLLTSFNKKSFLNSNPKFINNDGEKTIGFFV
HHHHHHEECCCCCCCCCEEEECCCHHHCCCCCCCCCHHHHCCCCCCEECCCCCHHHHHHH
GCLANYFYIDTANAVLKIAKELKINVDLMKEQVCCGAPQFFTGDFKSVEVLAKKNIEYFE
HHHHHHEEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHH
KKLEKLDAIIVPEATCSAMLKIDYEHFFIMQNDLDWAKRAKCVSSKIYMASEYFYKFTSL
HHHHHHCEEECCCHHHHHHEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEILKTKNKINYSITYHDPCHARKMQGVFKEPRELLKTNYHFVEMSNSNTCCGFGGVGMQ
HHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
TDYYDRALSVGLKKAQMIDESKASVVSAECSACRMQISNALEQNSSKVVFASPLELIAKA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHC
L
C
>Mature Secondary Structure
MKFSQISDACVKCGKCIPVCTIHEENRDEITSPRGFLDLLAAYKEGSLELDKEAKKVFES
CCCHHHHHHHHHCCCCCEEEEECCCCCHHCCCCHHHHHHHHHHCCCCCEECHHHHHHHHH
CFLCTNCVEVCPSKLRVDNAIEEVRYDIAKKFGIAWYKKLIFFFLRRRKILDLVAKLGYV
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQSCAFKIQNENENTGMKARFSMPFVKKGRLLTSFNKKSFLNSNPKFINNDGEKTIGFFV
HHHHHHEECCCCCCCCCEEEECCCHHHCCCCCCCCCHHHHCCCCCCEECCCCCHHHHHHH
GCLANYFYIDTANAVLKIAKELKINVDLMKEQVCCGAPQFFTGDFKSVEVLAKKNIEYFE
HHHHHHEEEECHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHH
KKLEKLDAIIVPEATCSAMLKIDYEHFFIMQNDLDWAKRAKCVSSKIYMASEYFYKFTSL
HHHHHHCEEECCCHHHHHHEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEILKTKNKINYSITYHDPCHARKMQGVFKEPRELLKTNYHFVEMSNSNTCCGFGGVGMQ
HHHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCC
TDYYDRALSVGLKKAQMIDESKASVVSAECSACRMQISNALEQNSSKVVFASPLELIAKA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]