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The map label for this gene is truA [H]

Identifier: 57237668

GI number: 57237668

Start: 852279

End: 853001

Strand: Direct

Name: truA [H]

Synonym: CJE0914

Alternate gene names: 57237668

Gene position: 852279-853001 (Clockwise)

Preceding gene: 57237667

Following gene: 57237671

Centisome position: 47.94

GC content: 30.84

Gene sequence:

>723_bases
ATGAAAATAAAAATTATATTTTCTTATGATGGCACTGCTTTTTTAGGTTCTGCTACTCAGCCTCATAAAAAAGGTGTTCA
AGATGCTTTAAGTGGGGCCTTATCGCATTTGGGTATTTTTTCACCTCTTTTGATGGCTTCTCGTACAGATAAAGGAGTAC
ATGCAAGCTATGCTGTAGCTAGTGTGGAGTGTGGAGATTATTTTACAAATTTAGAATATCTTCAAAAACAGCTTAATAAA
TTTTCCCATCCTTTTATCCATATCAAAAAAATTGAAAAAGTAAAAGATGATTTTGAAGTAAGATTTGATGTAAAAAGTAG
AGAATACCGCTATATATTTTCTCACTCTTCTTATAGTCCTTTTATGGCTTCTTATGTGCATTTTTATCCTAAATTTGACT
TAGATAAGGCTAACGAGCTTTTGGGTTTTTTTGTGGGAAAAAAGGATTTAAAGTTTTTTTGCAAAAGTGGAGGAGATAAT
AAAACGACTTTAAGAGAAATTTTTATTGCTAGAGCTTATGCTTATAAAGATTTTAGCATTTTTCATTTTAAAGCTAATGG
TTTTTTAAGAGGGCAAATTAGATTAAGTGTAGCAAGTGTTTTAAAGGTTTTAGAAGGAAAAATGAGTGAAAAAGAACTCA
AAGAACAGATTGAGGCCAAAAAGCAATACAATCACTTTTTAGCACCGCCAAATGGGCTTTATTTAAGCCGTATATGTTAT
TAA

Upstream 100 bases:

>100_bases
CTTTTTCTCAACCTCCAATTTTGGCTTGCCTTGGAATTTTTTCTTTAAGTTTATTTGTTTCGGCTTATTGTTTTAAGAAA
ATGATTTTGAGTCGTTATTG

Downstream 100 bases:

>100_bases
AAACTTTCATAGAAATTAAAGGCCTTTTGGGTTAAAATTTTTCTTATTTCTTCTTGATGTTCTTTACCTTTGGTTTCTAG
GGTAATTGAAATCATCGCAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKIKIIFSYDGTAFLGSATQPHKKGVQDALSGALSHLGIFSPLLMASRTDKGVHASYAVASVECGDYFTNLEYLQKQLNK
FSHPFIHIKKIEKVKDDFEVRFDVKSREYRYIFSHSSYSPFMASYVHFYPKFDLDKANELLGFFVGKKDLKFFCKSGGDN
KTTLREIFIARAYAYKDFSIFHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFLAPPNGLYLSRICY

Sequences:

>Translated_240_residues
MKIKIIFSYDGTAFLGSATQPHKKGVQDALSGALSHLGIFSPLLMASRTDKGVHASYAVASVECGDYFTNLEYLQKQLNK
FSHPFIHIKKIEKVKDDFEVRFDVKSREYRYIFSHSSYSPFMASYVHFYPKFDLDKANELLGFFVGKKDLKFFCKSGGDN
KTTLREIFIARAYAYKDFSIFHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFLAPPNGLYLSRICY
>Mature_240_residues
MKIKIIFSYDGTAFLGSATQPHKKGVQDALSGALSHLGIFSPLLMASRTDKGVHASYAVASVECGDYFTNLEYLQKQLNK
FSHPFIHIKKIEKVKDDFEVRFDVKSREYRYIFSHSSYSPFMASYVHFYPKFDLDKANELLGFFVGKKDLKFFCKSGGDN
KTTLREIFIARAYAYKDFSIFHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFLAPPNGLYLSRICY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=244, Percent_Identity=26.6393442622951, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 27544; Mature: 27544

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKIIFSYDGTAFLGSATQPHKKGVQDALSGALSHLGIFSPLLMASRTDKGVHASYAVA
CEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEE
SVECGDYFTNLEYLQKQLNKFSHPFIHIKKIEKVKDDFEVRFDVKSREYRYIFSHSSYSP
EEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEEEECCCEEEEEEECCCCCH
FMASYVHFYPKFDLDKANELLGFFVGKKDLKFFCKSGGDNKTTLREIFIARAYAYKDFSI
HHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
FHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFLAPPNGLYLSRICY
EEEECCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEECC
>Mature Secondary Structure
MKIKIIFSYDGTAFLGSATQPHKKGVQDALSGALSHLGIFSPLLMASRTDKGVHASYAVA
CEEEEEEEECCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEE
SVECGDYFTNLEYLQKQLNKFSHPFIHIKKIEKVKDDFEVRFDVKSREYRYIFSHSSYSP
EEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEEEEEEECCCEEEEEEECCCCCH
FMASYVHFYPKFDLDKANELLGFFVGKKDLKFFCKSGGDNKTTLREIFIARAYAYKDFSI
HHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEE
FHFKANGFLRGQIRLSVASVLKVLEGKMSEKELKEQIEAKKQYNHFLAPPNGLYLSRICY
EEEECCCEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA