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The map label for this gene is trmD [H]

Identifier: 57237015

GI number: 57237015

Start: 746930

End: 747634

Strand: Direct

Name: trmD [H]

Synonym: CJE0813

Alternate gene names: 57237015

Gene position: 746930-747634 (Clockwise)

Preceding gene: 57237014

Following gene: 57237016

Centisome position: 42.01

GC content: 29.93

Gene sequence:

>705_bases
ATGAAATTTAGTTTCGTTTCTTTGTTCCCTAATCTTATGGAATTTTACTTCCAAGATTCTATACTTGCAAGAGCAAAAGA
AAAAAAACTTTTCAAACTTAATTTTTATAATCCAAGAGATTTTTCTAAAAATTCCTATCATAAAGTCGATGATTATAAAA
TAGGTGGTGGAGCTGGACTTTTGATGCAAGCTGAACCTATGTATGAGGTGCTAAGAAGTATTCAAGAAAAAAAAGAAAAT
CCCTATTTTATTTTTTTAAATCCAAGTGGTAAAACCTTTAATCAAAAAGACGCCAAACGCTTAAGTAAAAAAGAACATAT
AGTTTTTGTATGTGGGCGCTATGAAGGTATAGATGAAAGAGTGCTTGAAATTTTTGCGAATGAAATTTTTAGTATAGGGG
ATTTTATTTTAACAGGAGGAGAATTACCTGCGCTTGTAATGTGTGATGCGATTTTAAGAAATGTTAACGGGGTTTTAGGA
AATATGGAAAGTTTGAAAGAAGAAAGCTTTGAAAATAATTTACTCGAAGCGCCTGCTTTTTCAAAACCTTTTATTTTTGA
GAAAAAAAATAAAAAATTTTATACTCCTTCAGAGTTTTTAAAGGGTAATCACGCTAGAATTGCAAGTTTAAAAACTACTT
TGGCGTCTTGCAAAACGAAATTTTTTCGTCCAGATTTATTTTTAGAGCATGAACGCAAAAAATAA

Upstream 100 bases:

>100_bases
TATTTTTTATTCCATATTTGGGTAAATTTGTTAAAAATATAGATATAGAAAAACGACAGATTTTTTGCACGCAAGATGCT
TTTTTAATTTTAGAAAATTC

Downstream 100 bases:

>100_bases
GGGATTTTTTATGAAAAATAAATATATAGAACAATTTGAAGCAAAACAAATTGAAGGTAAAAATGTTCCAGATTTTCGTG
CTGGTGATACTTTAAAGCTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKFSFVSLFPNLMEFYFQDSILARAKEKKLFKLNFYNPRDFSKNSYHKVDDYKIGGGAGLLMQAEPMYEVLRSIQEKKEN
PYFIFLNPSGKTFNQKDAKRLSKKEHIVFVCGRYEGIDERVLEIFANEIFSIGDFILTGGELPALVMCDAILRNVNGVLG
NMESLKEESFENNLLEAPAFSKPFIFEKKNKKFYTPSEFLKGNHARIASLKTTLASCKTKFFRPDLFLEHERKK

Sequences:

>Translated_234_residues
MKFSFVSLFPNLMEFYFQDSILARAKEKKLFKLNFYNPRDFSKNSYHKVDDYKIGGGAGLLMQAEPMYEVLRSIQEKKEN
PYFIFLNPSGKTFNQKDAKRLSKKEHIVFVCGRYEGIDERVLEIFANEIFSIGDFILTGGELPALVMCDAILRNVNGVLG
NMESLKEESFENNLLEAPAFSKPFIFEKKNKKFYTPSEFLKGNHARIASLKTTLASCKTKFFRPDLFLEHERKK
>Mature_234_residues
MKFSFVSLFPNLMEFYFQDSILARAKEKKLFKLNFYNPRDFSKNSYHKVDDYKIGGGAGLLMQAEPMYEVLRSIQEKKEN
PYFIFLNPSGKTFNQKDAKRLSKKEHIVFVCGRYEGIDERVLEIFANEIFSIGDFILTGGELPALVMCDAILRNVNGVLG
NMESLKEESFENNLLEAPAFSKPFIFEKKNKKFYTPSEFLKGNHARIASLKTTLASCKTKFFRPDLFLEHERKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=34.2105263157895, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27124; Mature: 27124

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSFVSLFPNLMEFYFQDSILARAKEKKLFKLNFYNPRDFSKNSYHKVDDYKIGGGAGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCEE
LMQAEPMYEVLRSIQEKKENPYFIFLNPSGKTFNQKDAKRLSKKEHIVFVCGRYEGIDER
EEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHH
VLEIFANEIFSIGDFILTGGELPALVMCDAILRNVNGVLGNMESLKEESFENNLLEAPAF
HHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
SKPFIFEKKNKKFYTPSEFLKGNHARIASLKTTLASCKTKFFRPDLFLEHERKK
CCCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKFSFVSLFPNLMEFYFQDSILARAKEKKLFKLNFYNPRDFSKNSYHKVDDYKIGGGAGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCEEECCCCEE
LMQAEPMYEVLRSIQEKKENPYFIFLNPSGKTFNQKDAKRLSKKEHIVFVCGRYEGIDER
EEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHH
VLEIFANEIFSIGDFILTGGELPALVMCDAILRNVNGVLGNMESLKEESFENNLLEAPAF
HHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCC
SKPFIFEKKNKKFYTPSEFLKGNHARIASLKTTLASCKTKFFRPDLFLEHERKK
CCCCEEECCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA