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The map label for this gene is pyk [H]

Identifier: 21226817

GI number: 21226817

Start: 861276

End: 862709

Strand: Direct

Name: pyk [H]

Synonym: MM_0715

Alternate gene names: 21226817

Gene position: 861276-862709 (Clockwise)

Preceding gene: 21226816

Following gene: 21226818

Centisome position: 21.03

GC content: 47.77

Gene sequence:

>1434_bases
ATGGAAATCCCGGATCATAAAACAAAGATCGTCTGTACAATAGGACCTGCCTCTTCTTCTGAAGAGATGATAAGGAAGCT
TATGCTTGCGGGCATGAACGTGGCAAGGATAAATTTTTCTCACGGAGACTTTGAGAGCCACTCAAGGGTTGTCCGAATTA
TCCGTAAGGTTGCAGATGAACTTGGCAGGACAATTGCAATCCTTGCAGACCTGCCTGGCCCGAAAATACGCATAGGCAAA
CTCGAAAAAGAGCCTGTTATGCTCCATAAAGGGAACCCGATAACCCTTACAATTGACGACACTCCGGGAAACGAAGAGAG
AATCCCTGTCAGTTACAAACAGCTTCCGGAAAGCGTAACCCCTGGGAGCCTTATCTACCTCAGTGACGGGTTCATACAGC
TCCTCTGCAAGGAGGTTACAGGAAAAGACGTTCTCTGTGAAGTCCTTATCGGAGGAGAGCTTTATTCGCACAAAGGGCTG
AACCTTCCAGGGGCAAAGATCTTTCTTGACGCTGTGACTGAAAAAGATTTCAGGATCCTGGAATTTGCCCTTGAAGAGGA
CATTGACACTTTCAGCATCTCTTTTGTAGAAAATGCAGAAGATATCCGAAAAGTAAGAAATTTTGCCGCTTCCAGAGGAA
AGCAGGTAAATATAGTATCAAAAATAGAACGCAGGCAGGCTGTAGAAAATATAGGAGAAATCCTTGACGAGACCGATGCC
CTTATGGTTGCAAGGGGAGACCTGGGAGTTGAAATTCCTATCCAGGAAGTCCCTTCGGTCCAGAAAGAACTTATCCAGAG
GGCAAAGCTCCTTGGGGTTCCTGTGATTACTGCAACCCATATGCTGGCTTCCATGACAGATAATATAAGACCCACGCGTG
CCGAAGCCACGGATGTTGCAAACGCTATCCTTGACGGCACGGATGCTGTTATGCTCTCGGAAGAAACAGCAGTCGGAAAC
TACCCCGTGGAAGCTGTCGAGATGATGGCAAAGATTGCAAAGACCACTGAAAACTGGCGCTCCCGGACTAAATGGGGGCT
TGACACAATGATCAAATCCATAACTGCCAGGGAGATGTCAGTGGATGAGGTAATCACCTTACAGGTTTATGAAGCCCTGC
AGAAACTGCCTGTTGCAGCCGTACTTACCCCGACAAGGAGCGGAGCGACTCCCCGCAGGATTTCGCGCTTTAATCCTGAC
CCCTGGATTCTGGCTTTCAGCCGCGTTCCTAAGACCTGCAGGTTCCTTTCCCTGTCTTACGGGGTTTATCCTGTTGCCGT
AAACGAATCTATTGAAAGCTGGGAAAAAGAGACAACGGAAAGAACAAAAGAATTGGGATTTGCGAAAAGTAAAGATCTCG
TGATTTTTACCCAGGGCCCTGCTTCCGGAAAACCCGGAGGTACGAATATGCTCAAGATCCTGACCCTTGACTGA

Upstream 100 bases:

>100_bases
TACCAGAAGGGCCTGGGCAGGATTTTTCTGAAAGCGCATGAAATAAATTCCAAATTTTTCAAAAAGGGATAAATCCAGAA
AAATTTGAGGCTGTATAACC

Downstream 100 bases:

>100_bases
AAAGGGCAGAACATTCCAGAATTGAGATAAGGAGATAAAACCTCAATTATCTCAGAAAGACTTTTTTATCCCGAATTCTT
TTTTTCAAAAGTTAGGGTAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADELGRTIAILADLPGPKIRIGK
LEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVTPGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGL
NLPGAKIFLDAVTEKDFRILEFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVANAILDGTDAVMLSEETAVGN
YPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMSVDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPD
PWILAFSRVPKTCRFLSLSYGVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD

Sequences:

>Translated_477_residues
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADELGRTIAILADLPGPKIRIGK
LEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVTPGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGL
NLPGAKIFLDAVTEKDFRILEFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVANAILDGTDAVMLSEETAVGN
YPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMSVDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPD
PWILAFSRVPKTCRFLSLSYGVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD
>Mature_477_residues
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADELGRTIAILADLPGPKIRIGK
LEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVTPGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGL
NLPGAKIFLDAVTEKDFRILEFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVANAILDGTDAVMLSEETAVGN
YPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMSVDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPD
PWILAFSRVPKTCRFLSLSYGVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=38.6178861788618, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=38.6178861788618, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI33286418, Length=492, Percent_Identity=38.4146341463415, Blast_Score=316, Evalue=4e-86,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=38.2892057026477, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=38.2892057026477, Blast_Score=310, Evalue=2e-84,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=40.6896551724138, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=40.6896551724138, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI310128736, Length=233, Percent_Identity=39.0557939914163, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI310128734, Length=233, Percent_Identity=39.0557939914163, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=37.3831775700935, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1787965, Length=468, Percent_Identity=41.6666666666667, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=40.2489626556017, Blast_Score=313, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=38.6694386694387, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=40.2061855670103, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=40.2061855670103, Blast_Score=308, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=40.2061855670103, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=40.2061855670103, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=38.5567010309278, Blast_Score=302, Evalue=8e-83,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=35.3305785123967, Blast_Score=277, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=39.9594320486815, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=39.9594320486815, Blast_Score=310, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24648966, Length=416, Percent_Identity=38.9423076923077, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24581235, Length=427, Percent_Identity=32.3185011709602, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=34.7826086956522, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52684; Mature: 52684

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADE
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRTIAILADLPGPKIRIGKLEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVT
HCCEEEEEECCCCCCEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCCCHHHHCCCCCC
PGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGLNLPGAKIFLDAVTEKDFRIL
CCEEEEECCHHHHHHHHHHCCCHHEEHEEECCEEECCCCCCCCCHHEEEEHHCCCCHHHH
EFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCCE
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVA
EEEEECCCCEECCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NAILDGTDAVMLSEETAVGNYPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMS
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
VDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPDPWILAFSRVPKTCRFLSLSY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCCCEEEEECCCCCHHEEEEEEC
GVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MEIPDHKTKIVCTIGPASSSEEMIRKLMLAGMNVARINFSHGDFESHSRVVRIIRKVADE
CCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
LGRTIAILADLPGPKIRIGKLEKEPVMLHKGNPITLTIDDTPGNEERIPVSYKQLPESVT
HCCEEEEEECCCCCCEEEECCCCCCEEEECCCEEEEEECCCCCCCCCCCCHHHHCCCCCC
PGSLIYLSDGFIQLLCKEVTGKDVLCEVLIGGELYSHKGLNLPGAKIFLDAVTEKDFRIL
CCEEEEECCHHHHHHHHHHCCCHHEEHEEECCEEECCCCCCCCCHHEEEEHHCCCCHHHH
EFALEEDIDTFSISFVENAEDIRKVRNFAASRGKQVNIVSKIERRQAVENIGEILDETDA
HHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHCCCCE
LMVARGDLGVEIPIQEVPSVQKELIQRAKLLGVPVITATHMLASMTDNIRPTRAEATDVA
EEEEECCCCEECCHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
NAILDGTDAVMLSEETAVGNYPVEAVEMMAKIAKTTENWRSRTKWGLDTMIKSITAREMS
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
VDEVITLQVYEALQKLPVAAVLTPTRSGATPRRISRFNPDPWILAFSRVPKTCRFLSLSY
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHCCCCCCEEEEECCCCCHHEEEEEEC
GVYPVAVNESIESWEKETTERTKELGFAKSKDLVIFTQGPASGKPGGTNMLKILTLD
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA