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The map label for this gene is cca

Identifier: 21226571

GI number: 21226571

Start: 592432

End: 593796

Strand: Direct

Name: cca

Synonym: MM_0469

Alternate gene names: 21226571

Gene position: 592432-593796 (Clockwise)

Preceding gene: 21226562

Following gene: 21226580

Centisome position: 14.46

GC content: 46.3

Gene sequence:

>1365_bases
ATGGATACAGATACCAGTATTTCAGAAGACCTGAAGCTTGCCGTCCTTGAAAAGATTAAACCCACGGAAGCCGAAAGGAA
AGAACTCATGGCTGTTCAGGATGAGCTTGCCGCAGAAGTAAAAGCAGCAGCAGAGAAACTTTGCGTGTCTGATATTTTTG
TGAAGATGGTGGGTTCTGCTGCAAGAGGCACCTGGCTCTCAGGAACTCACGATATTGATGTTTTTATCAGCTTTCCCGAA
GAAACTTCCAGAAAAGACCTGGAAATAAGAGGAATGGGAATTGCCAGGGAAGTTGCAAAACACGCGGAATATGCCGAAGA
CCGCCATGCTGAACACCCTTACCTGAATATTGTTTACAAAGGCTTTGATGTGGACCTTGTCCCTTGCTTCCGGGTCTGTT
CTGCATGCCAGCTAAAATCCGCAGTCGACAGGACACCTTTCCATAATGAATTCATAAAATCCCGCATAAAAGGTAGGGAA
GATGATGTCCTGCTCATGAAGCAGTTTATGCGCGGGGGCAGAGTCTACGGCTCGGAACTGAAAACACAGGGATTTTCAGG
CTACCTGACCGAACTCCTTATCATTTACTACGGCTCTTTCGAAAAAACCGTAAAAGCAGCCTCTTCATGGAAGCCCGGCA
AGAAAATAGATATTATGCAGCACTCGGAACTGGAGCACAGCGAACCCCTGGTAATGGTGGACCCGACAGACCCGAAGCGG
AATGTTGCAGCAGCCCTCTCTCTTGACAAATTCTGCATGTTCATAGACCACTGCAGGGAGTTTCTGAAAAGCCCGGAAAT
CAAATTCTTTTTCCCTGAATCTCCCCTGCCCATCGAGGATAAAGAGTTTCTTGAAAAACTGGAAAGCCGGAAAACTTCAC
AGCTTGCAATCGTCTTCGAGACGCCTGATGTTGTGGACGACGTGCTCTATCCGCAACTGTATAAAATGGAACAGGCTGCA
TCTTCCCTCCTCAGCGAATACGATTTTTCAGTGATCAAAACCGGCGTCTGGTCAGGGAAACCCCAAACCGTGGTTATGCT
CGAACTTATCTCAGGCACCCTCCCCAACGTGAAAAAGAGAACTGGTCCTCCGGTATGGGTCAGGGAACATGCTGAGAAGT
TCAAAGACAAGTATGAAGGAGCTGAAAATGTTTTTGGAGGATATATCGAAAACGGGAAGTACGTCTATGAAGTCCACCGA
AAATACACAACTGCAAAAGGGCTCCTTGAAGAACAGCTTCTAAACTGCTCCCTGGGAAAACAGGTTTATCAGAGCGTGAA
TAAAGGCTTTGAGGTTATTGAAAATGCGGAAATTTGCAGGTTAAAAGATCAGGATTTCAGGGTTTTTTTGAGGAAATGGG
TGTAA

Upstream 100 bases:

>100_bases
AATCATTATAAAATTAAGAATCACTGCAGATTCGAGAATAAATTGCATATTCAATAATTTACTGTCATAAACGATTAATA
ACGTATGGAGCCGTCCCGGC

Downstream 100 bases:

>100_bases
ATTTACTATTAAGTACAAATCATTCCTCAATATTCGATTTTTCATAGAGAAGAGTACTCAGGGAGCTGCCCCCCGAACTC
TTACACAGGATAGGTACTTT

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MDTDTSISEDLKLAVLEKIKPTEAERKELMAVQDELAAEVKAAAEKLCVSDIFVKMVGSAARGTWLSGTHDIDVFISFPE
ETSRKDLEIRGMGIAREVAKHAEYAEDRHAEHPYLNIVYKGFDVDLVPCFRVCSACQLKSAVDRTPFHNEFIKSRIKGRE
DDVLLMKQFMRGGRVYGSELKTQGFSGYLTELLIIYYGSFEKTVKAASSWKPGKKIDIMQHSELEHSEPLVMVDPTDPKR
NVAAALSLDKFCMFIDHCREFLKSPEIKFFFPESPLPIEDKEFLEKLESRKTSQLAIVFETPDVVDDVLYPQLYKMEQAA
SSLLSEYDFSVIKTGVWSGKPQTVVMLELISGTLPNVKKRTGPPVWVREHAEKFKDKYEGAENVFGGYIENGKYVYEVHR
KYTTAKGLLEEQLLNCSLGKQVYQSVNKGFEVIENAEICRLKDQDFRVFLRKWV

Sequences:

>Translated_454_residues
MDTDTSISEDLKLAVLEKIKPTEAERKELMAVQDELAAEVKAAAEKLCVSDIFVKMVGSAARGTWLSGTHDIDVFISFPE
ETSRKDLEIRGMGIAREVAKHAEYAEDRHAEHPYLNIVYKGFDVDLVPCFRVCSACQLKSAVDRTPFHNEFIKSRIKGRE
DDVLLMKQFMRGGRVYGSELKTQGFSGYLTELLIIYYGSFEKTVKAASSWKPGKKIDIMQHSELEHSEPLVMVDPTDPKR
NVAAALSLDKFCMFIDHCREFLKSPEIKFFFPESPLPIEDKEFLEKLESRKTSQLAIVFETPDVVDDVLYPQLYKMEQAA
SSLLSEYDFSVIKTGVWSGKPQTVVMLELISGTLPNVKKRTGPPVWVREHAEKFKDKYEGAENVFGGYIENGKYVYEVHR
KYTTAKGLLEEQLLNCSLGKQVYQSVNKGFEVIENAEICRLKDQDFRVFLRKWV
>Mature_454_residues
MDTDTSISEDLKLAVLEKIKPTEAERKELMAVQDELAAEVKAAAEKLCVSDIFVKMVGSAARGTWLSGTHDIDVFISFPE
ETSRKDLEIRGMGIAREVAKHAEYAEDRHAEHPYLNIVYKGFDVDLVPCFRVCSACQLKSAVDRTPFHNEFIKSRIKGRE
DDVLLMKQFMRGGRVYGSELKTQGFSGYLTELLIIYYGSFEKTVKAASSWKPGKKIDIMQHSELEHSEPLVMVDPTDPKR
NVAAALSLDKFCMFIDHCREFLKSPEIKFFFPESPLPIEDKEFLEKLESRKTSQLAIVFETPDVVDDVLYPQLYKMEQAA
SSLLSEYDFSVIKTGVWSGKPQTVVMLELISGTLPNVKKRTGPPVWVREHAEKFKDKYEGAENVFGGYIENGKYVYEVHR
KYTTAKGLLEEQLLNCSLGKQVYQSVNKGFEVIENAEICRLKDQDFRVFLRKWV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG1746

COG function: function code J; tRNA nucleotidyltransferase (CCA-adding enzyme)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_METMA (Q8PZM3)

Other databases:

- EMBL:   AE008384
- RefSeq:   NP_632493.1
- ProteinModelPortal:   Q8PZM3
- SMR:   Q8PZM3
- GeneID:   1478811
- GenomeReviews:   AE008384_GR
- KEGG:   mma:MM_0469
- NMPDR:   fig|192952.1.peg.469
- HOGENOM:   HBG645243
- OMA:   CEKIISA
- ProtClustDB:   PRK13300
- BioCyc:   MMAZ192952:MM0469-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01264
- InterPro:   IPR008229
- InterPro:   IPR002934
- InterPro:   IPR011068
- InterPro:   IPR015329
- PIRSF:   PIRSF005335
- TIGRFAMs:   TIGR03671

Pfam domain/function: PF01909 NTP_transf_2; PF09249 tRNA_NucTransf2; SSF55003 PAP_C

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 51768; Mature: 51768

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 62-62 BINDING 148-148 BINDING 167-167 BINDING 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTDTSISEDLKLAVLEKIKPTEAERKELMAVQDELAAEVKAAAEKLCVSDIFVKMVGSA
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ARGTWLSGTHDIDVFISFPEETSRKDLEIRGMGIAREVAKHAEYAEDRHAEHPYLNIVYK
HCCCCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GFDVDLVPCFRVCSACQLKSAVDRTPFHNEFIKSRIKGREDDVLLMKQFMRGGRVYGSEL
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEECHHH
KTQGFSGYLTELLIIYYGSFEKTVKAASSWKPGKKIDIMQHSELEHSEPLVMVDPTDPKR
HCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCH
NVAAALSLDKFCMFIDHCREFLKSPEIKFFFPESPLPIEDKEFLEKLESRKTSQLAIVFE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEC
TPDVVDDVLYPQLYKMEQAASSLLSEYDFSVIKTGVWSGKPQTVVMLELISGTLPNVKKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEHHHHHCCCCCCCHHH
TGPPVWVREHAEKFKDKYEGAENVFGGYIENGKYVYEVHRKYTTAKGLLEEQLLNCSLGK
CCCCHHHHHHHHHHHHHHCCHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHH
QVYQSVNKGFEVIENAEICRLKDQDFRVFLRKWV
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCC
>Mature Secondary Structure
MDTDTSISEDLKLAVLEKIKPTEAERKELMAVQDELAAEVKAAAEKLCVSDIFVKMVGSA
CCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
ARGTWLSGTHDIDVFISFPEETSRKDLEIRGMGIAREVAKHAEYAEDRHAEHPYLNIVYK
HCCCCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GFDVDLVPCFRVCSACQLKSAVDRTPFHNEFIKSRIKGREDDVLLMKQFMRGGRVYGSEL
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCEECHHH
KTQGFSGYLTELLIIYYGSFEKTVKAASSWKPGKKIDIMQHSELEHSEPLVMVDPTDPKR
HCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCH
NVAAALSLDKFCMFIDHCREFLKSPEIKFFFPESPLPIEDKEFLEKLESRKTSQLAIVFE
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEC
TPDVVDDVLYPQLYKMEQAASSLLSEYDFSVIKTGVWSGKPQTVVMLELISGTLPNVKKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEEHHHHHCCCCCCCHHH
TGPPVWVREHAEKFKDKYEGAENVFGGYIENGKYVYEVHRKYTTAKGLLEEQLLNCSLGK
CCCCHHHHHHHHHHHHHHCCHHHHCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHH
QVYQSVNKGFEVIENAEICRLKDQDFRVFLRKWV
HHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12125824