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The map label for this gene is hydN [H]

Identifier: 21226224

GI number: 21226224

Start: 152239

End: 152781

Strand: Direct

Name: hydN [H]

Synonym: MM_0122

Alternate gene names: 21226224

Gene position: 152239-152781 (Clockwise)

Preceding gene: 21226223

Following gene: 21226225

Centisome position: 3.72

GC content: 47.51

Gene sequence:

>543_bases
ATGAAAGACGTGATCATCCTGCCGGAACGCTGCATGGGCTGCCGCTCCTGTGAGGTTGCCTGTGCTGTTTCCCATTCAAA
AAGCAAGAACCTTTTCCAGGCTTTAGGAGAAGAAAAAGTCCCGAAAAAACGCATCTCTGTTGAATACATCCACGCACTTG
AAGCCTCAGTTCCTATCTCCTGCCGTCACTGCGAGGATGCACCCTGCGTTTCGGTTTGCCCCACAAAAGCTCTTTCCAAG
GACACAGTAACAGGTACTGTAAATCACAATAATGAGCTTTGCGTGGACTGCTGGAAATGTTCTACTGTATGCACCCGTTT
TTCGCCCCTGTATCTCTTACTCCTTGTTTCAGGGTGCTGGAGCTCTTCCATGTCACACAACTATGGAGTAATCGACAGGC
AGACTGAAGAAGGGACAATTGTAAGATGTGATCTATGCGGGGAAAGGGAATTACCTGCATGTGTTGAAGCCTGCCCAACA
CACGCTCTTGTATTTCTGGATATAAAGAGGCATCCGGAAACAAAGGCTTTTAGCTATTTCTGA

Upstream 100 bases:

>100_bases
TGCAGTTGAGCCTGACCCCTTCAAAGCCGCAAACCTGATGCTTGAGCACATTGAAGGAAAAAGAAAAGCATTGGGCCTGA
ACTGAGGGAGGAGGAACGTA

Downstream 100 bases:

>100_bases
AAACTCCAGGGCAAATTAAAAAATATACTGGAAAACATCATTTATGGTAATGGGTTTTCCAGGTTTTATTTTTCGTCGTG
AAACAGTAAAGTAGCCGGAT

Product: Iron-sulfur protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MKDVIILPERCMGCRSCEVACAVSHSKSKNLFQALGEEKVPKKRISVEYIHALEASVPISCRHCEDAPCVSVCPTKALSK
DTVTGTVNHNNELCVDCWKCSTVCTRFSPLYLLLLVSGCWSSSMSHNYGVIDRQTEEGTIVRCDLCGERELPACVEACPT
HALVFLDIKRHPETKAFSYF

Sequences:

>Translated_180_residues
MKDVIILPERCMGCRSCEVACAVSHSKSKNLFQALGEEKVPKKRISVEYIHALEASVPISCRHCEDAPCVSVCPTKALSK
DTVTGTVNHNNELCVDCWKCSTVCTRFSPLYLLLLVSGCWSSSMSHNYGVIDRQTEEGTIVRCDLCGERELPACVEACPT
HALVFLDIKRHPETKAFSYF
>Mature_180_residues
MKDVIILPERCMGCRSCEVACAVSHSKSKNLFQALGEEKVPKKRISVEYIHALEASVPISCRHCEDAPCVSVCPTKALSK
DTVTGTVNHNNELCVDCWKCSTVCTRFSPLYLLLLVSGCWSSSMSHNYGVIDRQTEEGTIVRCDLCGERELPACVEACPT
HALVFLDIKRHPETKAFSYF

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=165, Percent_Identity=34.5454545454545, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI87082179, Length=172, Percent_Identity=36.046511627907, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI2367245, Length=168, Percent_Identity=32.1428571428571, Blast_Score=84, Evalue=4e-18,
Organism=Escherichia coli, GI87082180, Length=165, Percent_Identity=29.0909090909091, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI1789079, Length=170, Percent_Identity=31.7647058823529, Blast_Score=74, Evalue=4e-15,
Organism=Escherichia coli, GI1788811, Length=159, Percent_Identity=30.188679245283, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI2367345, Length=163, Percent_Identity=30.0613496932515, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1787872, Length=182, Percent_Identity=29.1208791208791, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787122, Length=188, Percent_Identity=27.1276595744681, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789370, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI87082114, Length=204, Percent_Identity=28.921568627451, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 19976; Mature: 19976

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
11.1 %Cys+Met (Translated Protein)
9.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
11.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVIILPERCMGCRSCEVACAVSHSKSKNLFQALGEEKVPKKRISVEYIHALEASVPIS
CCCEEECCHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
CRHCEDAPCVSVCPTKALSKDTVTGTVNHNNELCVDCWKCSTVCTRFSPLYLLLLVSGCW
EECCCCCCCEEECCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHC
SSSMSHNYGVIDRQTEEGTIVRCDLCGERELPACVEACPTHALVFLDIKRHPETKAFSYF
CCCCCCCCCCEECCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKDVIILPERCMGCRSCEVACAVSHSKSKNLFQALGEEKVPKKRISVEYIHALEASVPIS
CCCEEECCHHHCCCCCCEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEE
CRHCEDAPCVSVCPTKALSKDTVTGTVNHNNELCVDCWKCSTVCTRFSPLYLLLLVSGCW
EECCCCCCCEEECCCCCCCCCCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHC
SSSMSHNYGVIDRQTEEGTIVRCDLCGERELPACVEACPTHALVFLDIKRHPETKAFSYF
CCCCCCCCCCEECCCCCCCEEEEECCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]