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The map label for this gene is pyrG [H]

Identifier: 21226220

GI number: 21226220

Start: 146550

End: 148154

Strand: Reverse

Name: pyrG [H]

Synonym: MM_0118

Alternate gene names: 21226220

Gene position: 148154-146550 (Counterclockwise)

Preceding gene: 21226221

Following gene: 21226219

Centisome position: 3.62

GC content: 48.04

Gene sequence:

>1605_bases
ATGAAGTATATCGTAGTTACCGGTGGGGTGATGAGCGGGCTTGGGAAAGGCATCACCATCGCGTCCATCGGCAGAAACCT
GAAAAATAAAGGTTATAAAGTTACGGCTATCAAGATCGACCCTTACATCAATATCGATGCAGGTACCATGAGCCCTTACC
AGCACGGGGAAGTTTTTGTGCTCAGGGACGGAGGCGAAGTTGACCTGGATCTCGGAAACTACGAGAGGTTCCTTGACACG
GAACTCACCAGGGACCACAACATAACAACTGGCAAGGTTTATCAGGAAGTGATCGCCAAAGAAAGGAGGGGAGACTACCT
CGGAAAAACCGTCCAGATTATTCCTCACATTACAAATGAGATCAAAAATAAAATAAGAAAAGTTGCAGCAAGGAGCGGGG
CTGATGTCTGTCTTATTGAGATAGGAGGGACTGTAGGAGACATTGAGAGCATGCCTTTCCTTGAAGCAGTCCGCCAGATG
CACAGGGAAGAACCTTCGGAAAACATTGTTTTTATCCATGTGACCCTTGTAATGGAAGATTTGCAGGGAGAACAGAAGAC
CAAACCTACGCAGCACTCGGTGAAAGAACTCCGGGCACTTGGGCTGAGCCCCGAAGTGATAGTTACAAGGTCAAAGACTC
CCCTCCAGGAAAGCGCCAAGGAAAAAATTGCCTTATTCTGTGATGTTCCCCAGGAGCTGGTTATCAGCGCCCATGATGCC
GGCGACATTTACGAAGTGCCTCTTGAGATCGAGGAGCAGGGACTGACCACCCAGCTTATGAAGCACCTGAGGCTGGAGTC
CGGTGTTGATGATGGTGGCTGGAGAGAAATGGTTGCAAGGATGAAGTCCACAACCGACGAGGTAAAGCTGGCAATCGTGG
GCAAATATACAAATCTCGAAGATTCCTATCTCAGCATCCTTGAAGCTGTAAAGCACGGAGGAATTGATAACGCGTGCAGG
GTTGAAGTCAACATGGTTGAAGCTGAGACCCTTGAAGAAGACCTGGTAGAAGTTGAAAAACTAAAACAGTATGATGGCAT
CCTGATTCCAGGCGGCTTCGGAGGCCGTGGAACTGAAGGGAAGATGCTTGCAATTAAATTTGCCAGGGAAAACGACATCC
CGTTCCTTGGAATCTGCCTGGGTATGCAGCTTGCGGTAATTGAGTTTGCAAGAAATGTGGCAAACCTTGAAAACGCAAAC
AGCACGGAGTTTGATGAAGATACTCCTTACCCTGTGATTGATATCCTCCCGGAACAGACCGGAGTTGCGGATATGGGAGG
CACCATGCGTCTTGGGGACTATGATGCTATCCTGAAAGAAGGCTCCTTTGCCACAAAGCTCTATGGGACCAATTATATTG
TTGAGCGTCACCGCCACAGGTATGAAGTAAACCCCGAATTCGTGGACAGGCTTGAATCCTTTGGCATAGTGTTTTCCGGC
AAAAATAAAAATAGAATGGAAATTGCCGAAATCCCAGGAAAACGATTTTTCTTTGCCTCCCAGTTCCACCCTGAGTTCAA
GTCGAGACCAGGCAGGCCGTCTCCTCCATTTAAAGGGCTCGTCAGGGCAATGTGTAAATACAGGAAGGAAAGAGAAGTTC
AGTAA

Upstream 100 bases:

>100_bases
AATTGTATAAGTTACACTTCTAAGTACTTTTTAATCTGACATTTACAGGACTGTATTCAGTCCGGAATTCTTATTAGAAA
ATTATCGAGGAAGCTTACCC

Downstream 100 bases:

>100_bases
GGACAGCATTTGCTGGATGACTCCGGAACTTTATAAAAACGTCAGTTTCCCTTTCTCGGGAAACTGATAAATAGTTTTAA
AAATGAAATTAATGGGTTAA

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MKYIVVTGGVMSGLGKGITIASIGRNLKNKGYKVTAIKIDPYINIDAGTMSPYQHGEVFVLRDGGEVDLDLGNYERFLDT
ELTRDHNITTGKVYQEVIAKERRGDYLGKTVQIIPHITNEIKNKIRKVAARSGADVCLIEIGGTVGDIESMPFLEAVRQM
HREEPSENIVFIHVTLVMEDLQGEQKTKPTQHSVKELRALGLSPEVIVTRSKTPLQESAKEKIALFCDVPQELVISAHDA
GDIYEVPLEIEEQGLTTQLMKHLRLESGVDDGGWREMVARMKSTTDEVKLAIVGKYTNLEDSYLSILEAVKHGGIDNACR
VEVNMVEAETLEEDLVEVEKLKQYDGILIPGGFGGRGTEGKMLAIKFARENDIPFLGICLGMQLAVIEFARNVANLENAN
STEFDEDTPYPVIDILPEQTGVADMGGTMRLGDYDAILKEGSFATKLYGTNYIVERHRHRYEVNPEFVDRLESFGIVFSG
KNKNRMEIAEIPGKRFFFASQFHPEFKSRPGRPSPPFKGLVRAMCKYRKEREVQ

Sequences:

>Translated_534_residues
MKYIVVTGGVMSGLGKGITIASIGRNLKNKGYKVTAIKIDPYINIDAGTMSPYQHGEVFVLRDGGEVDLDLGNYERFLDT
ELTRDHNITTGKVYQEVIAKERRGDYLGKTVQIIPHITNEIKNKIRKVAARSGADVCLIEIGGTVGDIESMPFLEAVRQM
HREEPSENIVFIHVTLVMEDLQGEQKTKPTQHSVKELRALGLSPEVIVTRSKTPLQESAKEKIALFCDVPQELVISAHDA
GDIYEVPLEIEEQGLTTQLMKHLRLESGVDDGGWREMVARMKSTTDEVKLAIVGKYTNLEDSYLSILEAVKHGGIDNACR
VEVNMVEAETLEEDLVEVEKLKQYDGILIPGGFGGRGTEGKMLAIKFARENDIPFLGICLGMQLAVIEFARNVANLENAN
STEFDEDTPYPVIDILPEQTGVADMGGTMRLGDYDAILKEGSFATKLYGTNYIVERHRHRYEVNPEFVDRLESFGIVFSG
KNKNRMEIAEIPGKRFFFASQFHPEFKSRPGRPSPPFKGLVRAMCKYRKEREVQ
>Mature_534_residues
MKYIVVTGGVMSGLGKGITIASIGRNLKNKGYKVTAIKIDPYINIDAGTMSPYQHGEVFVLRDGGEVDLDLGNYERFLDT
ELTRDHNITTGKVYQEVIAKERRGDYLGKTVQIIPHITNEIKNKIRKVAARSGADVCLIEIGGTVGDIESMPFLEAVRQM
HREEPSENIVFIHVTLVMEDLQGEQKTKPTQHSVKELRALGLSPEVIVTRSKTPLQESAKEKIALFCDVPQELVISAHDA
GDIYEVPLEIEEQGLTTQLMKHLRLESGVDDGGWREMVARMKSTTDEVKLAIVGKYTNLEDSYLSILEAVKHGGIDNACR
VEVNMVEAETLEEDLVEVEKLKQYDGILIPGGFGGRGTEGKMLAIKFARENDIPFLGICLGMQLAVIEFARNVANLENAN
STEFDEDTPYPVIDILPEQTGVADMGGTMRLGDYDAILKEGSFATKLYGTNYIVERHRHRYEVNPEFVDRLESFGIVFSG
KNKNRMEIAEIPGKRFFFASQFHPEFKSRPGRPSPPFKGLVRAMCKYRKEREVQ

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=51.9195612431444, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=51.9195612431444, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=51.9195612431444, Blast_Score=542, Evalue=1e-154,
Organism=Homo sapiens, GI148491070, Length=547, Percent_Identity=52.4680073126143, Blast_Score=541, Evalue=1e-154,
Organism=Escherichia coli, GI1789142, Length=546, Percent_Identity=48.5347985347985, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI25148299, Length=603, Percent_Identity=41.9568822553897, Blast_Score=464, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=50.3571428571429, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6319432, Length=557, Percent_Identity=50.2692998204668, Blast_Score=511, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24664469, Length=547, Percent_Identity=50.6398537477148, Blast_Score=526, Evalue=1e-149,
Organism=Drosophila melanogaster, GI21357815, Length=492, Percent_Identity=48.3739837398374, Blast_Score=446, Evalue=1e-125,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59719; Mature: 59719

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYIVVTGGVMSGLGKGITIASIGRNLKNKGYKVTAIKIDPYINIDAGTMSPYQHGEVFV
CEEEEEECHHHHHCCCCCCHHHHCCHHHCCCEEEEEEEECCEEEECCCCCCCCCCCCEEE
LRDGGEVDLDLGNYERFLDTELTRDHNITTGKVYQEVIAKERRGDYLGKTVQIIPHITNE
EECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IKNKIRKVAARSGADVCLIEIGGTVGDIESMPFLEAVRQMHREEPSENIVFIHVTLVMED
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEHH
LQGEQKTKPTQHSVKELRALGLSPEVIVTRSKTPLQESAKEKIALFCDVPQELVISAHDA
HCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCEEEEEECCCHHHEEEECCC
GDIYEVPLEIEEQGLTTQLMKHLRLESGVDDGGWREMVARMKSTTDEVKLAIVGKYTNLE
CCEEECCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DSYLSILEAVKHGGIDNACRVEVNMVEAETLEEDLVEVEKLKQYDGILIPGGFGGRGTEG
HHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
KMLAIKFARENDIPFLGICLGMQLAVIEFARNVANLENANSTEFDEDTPYPVIDILPEQT
CEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
GVADMGGTMRLGDYDAILKEGSFATKLYGTNYIVERHRHRYEVNPEFVDRLESFGIVFSG
CCCCCCCCEEECCHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCEEEEC
KNKNRMEIAEIPGKRFFFASQFHPEFKSRPGRPSPPFKGLVRAMCKYRKEREVQ
CCCCCEEHEECCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYIVVTGGVMSGLGKGITIASIGRNLKNKGYKVTAIKIDPYINIDAGTMSPYQHGEVFV
CEEEEEECHHHHHCCCCCCHHHHCCHHHCCCEEEEEEEECCEEEECCCCCCCCCCCCEEE
LRDGGEVDLDLGNYERFLDTELTRDHNITTGKVYQEVIAKERRGDYLGKTVQIIPHITNE
EECCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IKNKIRKVAARSGADVCLIEIGGTVGDIESMPFLEAVRQMHREEPSENIVFIHVTLVMED
HHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEEEEHH
LQGEQKTKPTQHSVKELRALGLSPEVIVTRSKTPLQESAKEKIALFCDVPQELVISAHDA
HCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHCEEEEEECCCHHHEEEECCC
GDIYEVPLEIEEQGLTTQLMKHLRLESGVDDGGWREMVARMKSTTDEVKLAIVGKYTNLE
CCEEECCCEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DSYLSILEAVKHGGIDNACRVEVNMVEAETLEEDLVEVEKLKQYDGILIPGGFGGRGTEG
HHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
KMLAIKFARENDIPFLGICLGMQLAVIEFARNVANLENANSTEFDEDTPYPVIDILPEQT
CEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCC
GVADMGGTMRLGDYDAILKEGSFATKLYGTNYIVERHRHRYEVNPEFVDRLESFGIVFSG
CCCCCCCCEEECCHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHCCEEEEC
KNKNRMEIAEIPGKRFFFASQFHPEFKSRPGRPSPPFKGLVRAMCKYRKEREVQ
CCCCCEEHEECCCCEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]