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The map label for this gene is iscS [H]

Identifier: 21226211

GI number: 21226211

Start: 135605

End: 136876

Strand: Direct

Name: iscS [H]

Synonym: MM_0109

Alternate gene names: 21226211

Gene position: 135605-136876 (Clockwise)

Preceding gene: 21226208

Following gene: 21226212

Centisome position: 3.31

GC content: 46.54

Gene sequence:

>1272_bases
GTGCCGGATAAAAATGAAAGCTTTTGTAGGGCACACAATAAATTCATATCAGGCGAACTCCTATTTTCCTTGGGTGTTCT
GATGGGAGAGAACCGTTTAGTATACATGGACCACGCAGCCACCACTTTCATAAAACAGGAAGTGGTTGAAACTATGCTGC
CTTTTTTGAAAGACCATTTCGGGAATCCTTCTTCCCTGTATTCAATAGGCAGGGAAGGAAAAGAAGCTGTGGAAACTGCG
AGGGAAAAGCTTGCAAAGGCTCTTGGAGCCAGCCCCGAAGAAATTTACTTCACTTCAGGGGGGACGGAGTCTGACAACTG
GGCTGTTAAAGGAACTGCTTTTGCCAGGCGAAAAAAAGGGAAACATATTATCACCATACCAATCGAACACCACGCCGTGC
TTTACCCCTGCAAGTATCTTGAAACTAAGGGATTTGATGTAACCTATCTGCCTGTGGACGAATACGGACTTGTAGACCCT
GCAGAACTGGAAGCTGCAATAAGAGAAGACACTATCCTTATTTCGGTCATGTACGCCAATAATGAAATAGGGACAATCGA
GCCCATATTAGAGATAGGGAAGGTTGCCGGAGAGCACGGGGTTCCTTTCCATACCGATGCCGTACAGGCAATAGGCAATT
TTTCTCTTGACCTGAAGGGGAAAGACAGGGATGTGAATATGCTCTCACTTTCCGCGCATAAATTCTATGGGCCAAAAGGA
ATAGGGGCTCTATATATTAGAGAAGGGACTGAAATTGACAGCTACATTCACGGAGGAGCACAGGAAAAAAGGAAACGGGC
GGGGACAGAAAATGTTGCGGGAATTGCCGGAATGGGAAAGGCTATAGAGCTTGCGACTGCAAATATCGAGAAGCATGCTG
AAAAGATAAGAAAAATGAGGGACCGTTTAAGGAAAGGAATAATGGAAATTCCCCACTGCAGGCTGAACGGGCACCCGGAA
AGGCGCCTCCCGGGAAACCTGAACTTCAGTTTTGAGTATATAGAAGGGGAGTCCCTGCTGCTTATGCTTGACCAGATGGG
TATTTGCAGTTCAACAGGCAGTGCCTGTTCTTCTGGCTCTCTTGACCCTTCACATGTACTCAGCGCAATAGGAGTGCCCC
CGGAAATTGCCCAGGGCTCTTTGCGCCTGACTCTGGGAGATGCAAATACCGAAGAAGATGTCGATTACGTACTCGAAGTC
CTGCCTCAGGTCGTGGAAAAATTAAGGGCTATATCTCCATTCTATAAACCATCAGATGTATGTGAAAAATGA

Upstream 100 bases:

>100_bases
CTTCTCGATAAGGAAAAGTTGTTACAGTGTACTCAGGATTACAGACCGGGACGTTAACTCAAAGAATTATCCGGATTCCT
GAAGAATAGCGGGTTTCGCA

Downstream 100 bases:

>100_bases
ATACAAAGGACGCAAGTGGGGGAATTTTGTGTATAATAAGAAAGTAATGGACCATTTTATGAATCCGAGAAACGTGGGGG
AAATAGAAAATGCGGATGGC

Product: cysteine desulfhydrase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MPDKNESFCRAHNKFISGELLFSLGVLMGENRLVYMDHAATTFIKQEVVETMLPFLKDHFGNPSSLYSIGREGKEAVETA
REKLAKALGASPEEIYFTSGGTESDNWAVKGTAFARRKKGKHIITIPIEHHAVLYPCKYLETKGFDVTYLPVDEYGLVDP
AELEAAIREDTILISVMYANNEIGTIEPILEIGKVAGEHGVPFHTDAVQAIGNFSLDLKGKDRDVNMLSLSAHKFYGPKG
IGALYIREGTEIDSYIHGGAQEKRKRAGTENVAGIAGMGKAIELATANIEKHAEKIRKMRDRLRKGIMEIPHCRLNGHPE
RRLPGNLNFSFEYIEGESLLLMLDQMGICSSTGSACSSGSLDPSHVLSAIGVPPEIAQGSLRLTLGDANTEEDVDYVLEV
LPQVVEKLRAISPFYKPSDVCEK

Sequences:

>Translated_423_residues
MPDKNESFCRAHNKFISGELLFSLGVLMGENRLVYMDHAATTFIKQEVVETMLPFLKDHFGNPSSLYSIGREGKEAVETA
REKLAKALGASPEEIYFTSGGTESDNWAVKGTAFARRKKGKHIITIPIEHHAVLYPCKYLETKGFDVTYLPVDEYGLVDP
AELEAAIREDTILISVMYANNEIGTIEPILEIGKVAGEHGVPFHTDAVQAIGNFSLDLKGKDRDVNMLSLSAHKFYGPKG
IGALYIREGTEIDSYIHGGAQEKRKRAGTENVAGIAGMGKAIELATANIEKHAEKIRKMRDRLRKGIMEIPHCRLNGHPE
RRLPGNLNFSFEYIEGESLLLMLDQMGICSSTGSACSSGSLDPSHVLSAIGVPPEIAQGSLRLTLGDANTEEDVDYVLEV
LPQVVEKLRAISPFYKPSDVCEK
>Mature_422_residues
PDKNESFCRAHNKFISGELLFSLGVLMGENRLVYMDHAATTFIKQEVVETMLPFLKDHFGNPSSLYSIGREGKEAVETAR
EKLAKALGASPEEIYFTSGGTESDNWAVKGTAFARRKKGKHIITIPIEHHAVLYPCKYLETKGFDVTYLPVDEYGLVDPA
ELEAAIREDTILISVMYANNEIGTIEPILEIGKVAGEHGVPFHTDAVQAIGNFSLDLKGKDRDVNMLSLSAHKFYGPKGI
GALYIREGTEIDSYIHGGAQEKRKRAGTENVAGIAGMGKAIELATANIEKHAEKIRKMRDRLRKGIMEIPHCRLNGHPER
RLPGNLNFSFEYIEGESLLLMLDQMGICSSTGSACSSGSLDPSHVLSAIGVPPEIAQGSLRLTLGDANTEEDVDYVLEVL
PQVVEKLRAISPFYKPSDVCEK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=389, Percent_Identity=45.7583547557841, Blast_Score=343, Evalue=1e-94,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=37.1084337349398, Blast_Score=235, Evalue=6e-62,
Organism=Escherichia coli, GI48994898, Length=388, Percent_Identity=45.360824742268, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1787970, Length=393, Percent_Identity=26.2086513994911, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1789175, Length=395, Percent_Identity=27.0886075949367, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25143064, Length=393, Percent_Identity=42.2391857506361, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17533177, Length=326, Percent_Identity=31.2883435582822, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=44.2159383033419, Blast_Score=323, Evalue=3e-89,
Organism=Drosophila melanogaster, GI20129463, Length=407, Percent_Identity=42.014742014742, Blast_Score=308, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 46495; Mature: 46363

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDKNESFCRAHNKFISGELLFSLGVLMGENRLVYMDHAATTFIKQEVVETMLPFLKDHF
CCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHC
GNPSSLYSIGREGKEAVETAREKLAKALGASPEEIYFTSGGTESDNWAVKGTAFARRKKG
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECHHHHHHCCC
KHIITIPIEHHAVLYPCKYLETKGFDVTYLPVDEYGLVDPAELEAAIREDTILISVMYAN
CEEEEEEECCCEEEEEHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEEEC
NEIGTIEPILEIGKVAGEHGVPFHTDAVQAIGNFSLDLKGKDRDVNMLSLSAHKFYGPKG
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECHHHHCCCCC
IGALYIREGTEIDSYIHGGAQEKRKRAGTENVAGIAGMGKAIELATANIEKHAEKIRKMR
CCEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHH
DRLRKGIMEIPHCRLNGHPERRLPGNLNFSFEYIEGESLLLMLDQMGICSSTGSACSSGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCC
LDPSHVLSAIGVPPEIAQGSLRLTLGDANTEEDVDYVLEVLPQVVEKLRAISPFYKPSDV
CCHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
CEK
HCC
>Mature Secondary Structure 
PDKNESFCRAHNKFISGELLFSLGVLMGENRLVYMDHAATTFIKQEVVETMLPFLKDHF
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHC
GNPSSLYSIGREGKEAVETAREKLAKALGASPEEIYFTSGGTESDNWAVKGTAFARRKKG
CCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECHHHHHHCCC
KHIITIPIEHHAVLYPCKYLETKGFDVTYLPVDEYGLVDPAELEAAIREDTILISVMYAN
CEEEEEEECCCEEEEEHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEEEEC
NEIGTIEPILEIGKVAGEHGVPFHTDAVQAIGNFSLDLKGKDRDVNMLSLSAHKFYGPKG
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCCCCEEEEECHHHHCCCCC
IGALYIREGTEIDSYIHGGAQEKRKRAGTENVAGIAGMGKAIELATANIEKHAEKIRKMR
CCEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCHHCCCCEEEEHHHHHHHHHHHHHHHH
DRLRKGIMEIPHCRLNGHPERRLPGNLNFSFEYIEGESLLLMLDQMGICSSTGSACSSGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCCC
LDPSHVLSAIGVPPEIAQGSLRLTLGDANTEEDVDYVLEVLPQVVEKLRAISPFYKPSDV
CCHHHHHHHCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
CEK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]