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The map label for this gene is ilvB [H]

Identifier: 20094672

GI number: 20094672

Start: 1221500

End: 1222936

Strand: Reverse

Name: ilvB [H]

Synonym: MK1236

Alternate gene names: 20094672

Gene position: 1222936-1221500 (Counterclockwise)

Preceding gene: 20094673

Following gene: 20094671

Centisome position: 72.15

GC content: 70.77

Gene sequence:

>1437_bases
GTGACCTCGTTCAGGTTACGTCCCGTGTTCGGGGTACCCGGGGACGACGTGACCCGACCGTACCTGAGGCTCGCGGAGGA
GGACGAGCTAGTCCGGACGACGCGGGAGGACGCGGCCGTGTACGCGGCGATCGGGGCCGCCGAGGTCTCGGGCGAGCCGG
CGGCGGCCCTGGCGACCTGCGGGCCCGGGGTGGCGGTCGCGCTGGCGGGGCTGGCGTGCGCGCTGGCCGATCGCGTACCC
GTGCTCTGCGCGACCGGCGAGATCACCGAGCGCGGGTTCCAGTCGCTCGTACCCTCGATGGCGTCGGACGGCGTGGAGCT
CACGGTGACGGACGACCCGGAGGAAGCCGCACGGACCCTAGCGCGCTCAGAGCCCGTGCTGTACGTCGATCCGGGGTTCG
ACCCAGACGGGGTCTCCGCGGAGCTCGAGGGAATCGAGCCCGTCGAGCCGGACCCCGAGGACGTGGAGCGCGTCCGCGGG
CTCCTGGAGGGTAAGCGCGTGGCGATCCTGGTGGGACGGGGCTGCTACCGCGCGCGGTGTGGGGAGCTCGTGGAGCGGTT
GTGCGAGGTCCTGGAAGCACCGATCGTGGAGACGATGCGCGGCCGGGGCGTGGTGCCCGAGTACCACCCACGTAACGCCG
GGGTCGTGGGTCTCAGGGGCTGGGCGAACGACATGGTAGAGGACGCCGACGTCGTGCTCGCCCTAGGGGCCCGGTTGTCG
GGACGCACGCGCGCGGACATCGACTGGCCCACCACGGTCGCGGTCGGTTTCGGGGTGGAGGACGCGGACGAGGTCGTGGA
GTGCGACGTGCGGGCCTTCGTGGAGGCACTGCTCGAGGACCCTCCGAGGACGGAACCGTGGAACCCGGAGCCGGGCGAGC
CGCCGGAGTTCGATCTGGAGCTGTACCGGGTCGTGAAGGCCGCACTGAAGGCCTGGATCGGGCCGGCCACGGTGGACTCG
GGTCAGATCACCCCCACCGCGCTGCTGGCCGCCCGGCTGTCGTCCCCGTCCGAGTTGATCCACTCCGGGTCGTTCTGTCC
CATGGGGTTCGCGATCCCGGCGGCGCTGGGAGCCTCGGCCCGGGTCCCCGAGATGGCGCTCGCCCTGACCGGGGACGGTG
GGTTCATGGTCTCCTGTCAGGAGCTGGAGACGGCGGTCAGGGAGGACCTCCCGATCGCGGTGCTCGTCACCCGTAACGGG
GAGTTAGGGTTCACCCGGCAGGTGATGGAAGAGAAGGAGGGCAGGGCCGCCAAGATGGGGCTGGACGCCGACGTTCTGAG
TATAGTCGAGTCCCTGGGCGCCGAGGTCGAGGTGATCGAGAAACCGGACCCCGGTGAGGTGAAAAAGGTCCTCAGGGACG
TGGAGATGAACGGGGAAACCACCGTCGTGGTCGTCGAGCTCCCGCGCGAGGACGTCCCGATGCCGGGGTCGGGATGA

Upstream 100 bases:

>100_bases
TCGAGCGGTGGGCCCGGGAGCTCGGGCTCCGTTTCATGCCCGCGTGCTCCTTCCCCGATATGATCGAGGAGTACTTGAGA
ATGTCGAGGGAGGTGGAAGG

Downstream 100 bases:

>100_bases
GGAGAACCCCGGTCACCGAGCTGGTGAGGAGGGACAGGCGCCTCGACCGGTCGGAACGACGACGCCCGGATCCGACCCCG
GTCCTGACGATGATGAGTAC

Product: acetolactate synthase large subunit-like protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATCGPGVAVALAGLACALADRVP
VLCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTLARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRG
LLEGKRVAILVGRGCYRARCGELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLS
GRTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLELYRVVKAALKAWIGPATVDS
GQITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASARVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNG
ELGFTRQVMEEKEGRAAKMGLDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG

Sequences:

>Translated_478_residues
MTSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATCGPGVAVALAGLACALADRVP
VLCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTLARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRG
LLEGKRVAILVGRGCYRARCGELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLS
GRTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLELYRVVKAALKAWIGPATVDS
GQITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASARVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNG
ELGFTRQVMEEKEGRAAKMGLDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG
>Mature_477_residues
TSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATCGPGVAVALAGLACALADRVPV
LCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTLARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRGL
LEGKRVAILVGRGCYRARCGELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLSG
RTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLELYRVVKAALKAWIGPATVDSG
QITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASARVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNGE
LGFTRQVMEEKEGRAAKMGLDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=521, Percent_Identity=26.8714011516315, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1790104, Length=517, Percent_Identity=24.5647969052224, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=26.5799256505576, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1786717, Length=511, Percent_Identity=24.0704500978474, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 50756; Mature: 50625

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATC
CCCCEECEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHEEEEEECHHHCCCCCCHHHHHC
GPGVAVALAGLACALADRVPVLCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTL
CCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
ARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRGLLEGKRVAILVGRGCYRARC
HHCCCEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
GELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLS
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEECCCCC
GRTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLE
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
LYRVVKAALKAWIGPATVDSGQITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASA
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCC
RVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNGELGFTRQVMEEKEGRAAKMG
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHCC
LDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG
CCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TSFRLRPVFGVPGDDVTRPYLRLAEEDELVRTTREDAAVYAAIGAAEVSGEPAAALATC
CCCEECEECCCCCCCCCCHHHHHCCCHHHHHHHHHHHEEEEEECHHHCCCCCCHHHHHC
GPGVAVALAGLACALADRVPVLCATGEITERGFQSLVPSMASDGVELTVTDDPEEAARTL
CCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
ARSEPVLYVDPGFDPDGVSAELEGIEPVEPDPEDVERVRGLLEGKRVAILVGRGCYRARC
HHCCCEEEECCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
GELVERLCEVLEAPIVETMRGRGVVPEYHPRNAGVVGLRGWANDMVEDADVVLALGARLS
HHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHEECCCCC
GRTRADIDWPTTVAVGFGVEDADEVVECDVRAFVEALLEDPPRTEPWNPEPGEPPEFDLE
CCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
LYRVVKAALKAWIGPATVDSGQITPTALLAARLSSPSELIHSGSFCPMGFAIPAALGASA
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCHHHHHHCCCCC
RVPEMALALTGDGGFMVSCQELETAVREDLPIAVLVTRNGELGFTRQVMEEKEGRAAKMG
CCCCEEEEEECCCCEEEEHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCHHHCC
LDADVLSIVESLGAEVEVIEKPDPGEVKKVLRDVEMNGETTVVVVELPREDVPMPGSG
CCHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]