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The map label for this gene is LeuC_2

Identifier: 20094644

GI number: 20094644

Start: 1198443

End: 1199651

Strand: Reverse

Name: LeuC_2

Synonym: MK1208

Alternate gene names: 20094644

Gene position: 1199651-1198443 (Counterclockwise)

Preceding gene: 20094645

Following gene: 20094643

Centisome position: 70.78

GC content: 68.65

Gene sequence:

>1209_bases
GTGCCGTCCGTCGCGGAGAGGATCCTCTCGGAGAAGGTTGGAGAGCCCGTCGAGGCCGGCGAGACCGTGTACGTGGAGCC
CGACGTAATCATGCTGCACGACGGGTCCGGTGCGACCGCCCTCAGGACGCTGCGGGAGCTCGGTGTGGAGCGCGTCGAGA
GTCCGGAGAAAGTCGTTCTGATCTTCGATCACTCCGTACCCCCCAGCTCGGTAGAGGCGGCGAACCGCCAGAACGAGCTC
CTCGAGTTCGCGCGCCGCCACAGGATCGAGCACGTCCACGTGGACGAGGGCGTCTGCCATCAGGTGCTCGTCGAGGAGGG
GTACGCGGGACCCGGTCGGGTGGTCTTCGGCGGCGACTCCCACACCCCTACCTCGGGAGCCGCCTCCGCCCTAGCGTTCG
GGTTCGGAGGCACGGACATGGCCTTCGCCCTCCTCTACGGCGAGCTCTGGATCCGGGTCCCGAGGACCGTGCGGGTGCAC
GTCGAGGGCGAGCTGGAGGAACCCGCCACCGCCAAGGACCTGGCCCTGACCGTGGTCGGCGAGCTGGGCGCAGGGTACGC
GGACTACGCGGTCCTCGAGTACACGGGGCTCCCCGAGCGTATGTCGCTCGGCGACCGTATGTGCCTGTGTAATCTCGCCA
CGGAGGCCGGGGCGAAGTCCGCGTACGTGCCCCCGAAGGAGGGCCCTGAGGAGCTCCGACCCGGGGACGCGGACGAGGTG
ATCGAGCTGGACGCCTCCGAGGTCGAGCCCGTGGTCTCGGTCCCACACCGCGTCGACGACGTGCGGCCAGTGGGCGACGT
TCAAGGCGTGGAGGTGACCCGGGTGTTCGTGGGGTCCTGCACCAACGGTCGCTACAGGGACGTGAGGGTCTTCACCGAGA
TTCTGGAGGAACTTGACGGTCCTCACCCCGATGTGCGGATCGTGGTCGTGCCCGCGTCCCGCCGCGTGCTCGAGCGTATG
ACGGAGGCCGGGCTGACCCTGAAACTGATCCGTATGGGCGTCATGATCGCCCCACCCGGCTGCGGACCCTGCTTAGGCGA
GCACCTCGGAGTGCTCGGGGACGACGACGTCTGCGTCTCCACGGCAAACCGGAACTTCCCGGGTAGGATGGGCTCCCGCA
GGGCCGAGATATACCTGGCGAGTCCCGTGACGGCCGCTGTCGCCGCGGCGGAGGGCGAGCTGGTCGATCCCCAAGACGTC
CTAGGGTGA

Upstream 100 bases:

>100_bases
ACCACCTCGCACAACGCCCGACGGAGGAGCTTCCTGCAGTCCTCCTGCCGGGACCGTATCACGATCAATCCCGTCCATCC
CCCGGAGGGGGGAAGCGAAC

Downstream 100 bases:

>100_bases
GCGTCTTGAGCGAACGGCTGAACCGGTTCGCATACAAGTTCCTCGATATCACGGTACATATCGTAATGATCTCGATTCTC
GCCATCACCCTCTTCATCTT

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MPSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVLIFDHSVPPSSVEAANRQNEL
LEFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDSHTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVH
VEGELEEPATAKDLALTVVGELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEV
IELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDGPHPDVRIVVVPASRRVLERM
TEAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVSTANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDV
LG

Sequences:

>Translated_402_residues
MPSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVLIFDHSVPPSSVEAANRQNEL
LEFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDSHTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVH
VEGELEEPATAKDLALTVVGELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEV
IELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDGPHPDVRIVVVPASRRVLERM
TEAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVSTANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDV
LG
>Mature_401_residues
PSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVLIFDHSVPPSSVEAANRQNELL
EFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDSHTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVHV
EGELEEPATAKDLALTVVGELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEVI
ELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDGPHPDVRIVVVPASRRVLERMT
EAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVSTANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDVL
G

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=24.3847874720358, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786259, Length=448, Percent_Identity=29.6875, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI2367097, Length=136, Percent_Identity=36.7647058823529, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787531, Length=492, Percent_Identity=25.609756097561, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=447, Percent_Identity=24.8322147651007, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI32564738, Length=338, Percent_Identity=27.810650887574, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25149342, Length=285, Percent_Identity=25.6140350877193, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=431, Percent_Identity=28.3062645011601, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321429, Length=359, Percent_Identity=28.9693593314763, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=463, Percent_Identity=24.8380129589633, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6323335, Length=447, Percent_Identity=24.3847874720358, Blast_Score=123, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28571643, Length=446, Percent_Identity=24.8878923766816, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=23.7136465324385, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=23.7136465324385, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161076999, Length=413, Percent_Identity=24.2130750605327, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC2_METKA (Q8TW29)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614491.1
- ProteinModelPortal:   Q8TW29
- SMR:   Q8TW29
- GeneID:   1477803
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1208
- NMPDR:   fig|190192.1.peg.1204
- HOGENOM:   HBG330745
- OMA:   PSIEIAN
- BioCyc:   MKAN190192:MK1208-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 43096; Mature: 42965

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVL
CCHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCHHHHCCCCEEEE
IFDHSVPPSSVEAANRQNELLEFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDS
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCEEEECCCC
HTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVHVEGELEEPATAKDLALTVVG
CCCCCCHHHHHEECCCCHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHEEEEH
ELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEV
HHCCCCCCEEEEEECCCCHHHCCCCCEEEEEHHHHCCCCCCCCCCCCCHHHCCCCCHHHE
IELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDG
EECCHHHCCHHHHCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCC
PHPDVRIVVVPASRRVLERMTEAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVS
CCCCEEEEEECCHHHHHHHHHHCCCEEEEEHCCEEECCCCCCHHHHHHCCCCCCCCEEEE
TANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDVLG
ECCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHCC
>Mature Secondary Structure 
PSVAERILSEKVGEPVEAGETVYVEPDVIMLHDGSGATALRTLRELGVERVESPEKVVL
CHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHCHHHHCCCCEEEE
IFDHSVPPSSVEAANRQNELLEFARRHRIEHVHVDEGVCHQVLVEEGYAGPGRVVFGGDS
EEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCCCCEEEECCCC
HTPTSGAASALAFGFGGTDMAFALLYGELWIRVPRTVRVHVEGELEEPATAKDLALTVVG
CCCCCCHHHHHEECCCCHHHHHHHHHHHHHEECCCEEEEEEECCCCCCCCHHHHHEEEEH
ELGAGYADYAVLEYTGLPERMSLGDRMCLCNLATEAGAKSAYVPPKEGPEELRPGDADEV
HHCCCCCCEEEEEECCCCHHHCCCCCEEEEEHHHHCCCCCCCCCCCCCHHHCCCCCHHHE
IELDASEVEPVVSVPHRVDDVRPVGDVQGVEVTRVFVGSCTNGRYRDVRVFTEILEELDG
EECCHHHCCHHHHCCCHHCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCC
PHPDVRIVVVPASRRVLERMTEAGLTLKLIRMGVMIAPPGCGPCLGEHLGVLGDDDVCVS
CCCCEEEEEECCHHHHHHHHHHCCCEEEEEHCCEEECCCCCCHHHHHHCCCCCCCCEEEE
TANRNFPGRMGSRRAEIYLASPVTAAVAAAEGELVDPQDVLG
ECCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014