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The map label for this gene is LeuD_2

Identifier: 20094642

GI number: 20094642

Start: 1197474

End: 1197980

Strand: Reverse

Name: LeuD_2

Synonym: MK1206

Alternate gene names: 20094642

Gene position: 1197980-1197474 (Counterclockwise)

Preceding gene: 20094643

Following gene: 20094641

Centisome position: 70.68

GC content: 66.86

Gene sequence:

>507_bases
GTGAGGTGGAGCGGTAGAGCGCACGTGTTCGGCGACGACGTCGACACCGACCAGATCATCCCCGGGCGTTGCCTGAGGAG
GGTCTCCTACGACGAGCTCGGCCGCTACGCCATGACGGGGGCGGATCCCGAGTTCCCCGAGAAGGTCCGCGAGGGCGACG
TGATCGTGGCCGGGAAGAACTTCGGCTGCGGTTCCTCCCGTGAGCAGGCAGTTATGGCCCTCCAGCAGGCCGGGGTCGCC
TGCGTGGTCGCGAGATCCTTCGCTCGTATCTTCTACCGGAACGCGATCAACCGTGGGCTGCCCACCGTCGAAGCCGAAGA
GGATCCCACCGAGGTAGTGGAGGACGGTAACCGGGTCACCGTGGACCTAGACGAGCTGGTCCTCCGGGCGGGGTCGGAGG
AGGTACCGCTGCGCGAGCCTCCCGAGTTCGCGCTGCAGGCCTGGAGGGAGGGTGGCCTGCTAGAGCTGGTCAAGAAGAAC
CCCGATAAGCCGCCCTGGAGGGACTGA

Upstream 100 bases:

>100_bases
ACCGCCATGTTGACCGTACTCATCGCGTCGCGCGTCGTAGTTCTCAAGTACTCACCCGACTACTTCAAAACGCACCTCGA
GAGGATACGGGGGCAGGAGC

Downstream 100 bases:

>100_bases
TCTCCGGCCCGCCACTCGGACTCCGATCGTCTACGAAACGTTATGGTGATCGAAACGTTTGGGAGGTCGAGGATGGAGGG
CCTCCTGACGATCGTACGCC

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKNFGCGSSREQAVMALQQAGVA
CVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVTVDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKN
PDKPPWRD

Sequences:

>Translated_168_residues
MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKNFGCGSSREQAVMALQQAGVA
CVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVTVDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKN
PDKPPWRD
>Mature_168_residues
MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKNFGCGSSREQAVMALQQAGVA
CVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVTVDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKN
PDKPPWRD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=163, Percent_Identity=31.9018404907975, Blast_Score=67, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320440, Length=96, Percent_Identity=44.7916666666667, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=131, Percent_Identity=32.824427480916, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD2_METKA (Q8TW31)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614489.1
- ProteinModelPortal:   Q8TW31
- SMR:   Q8TW31
- GeneID:   1477801
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1206
- NMPDR:   fig|190192.1.peg.1202
- HOGENOM:   HBG304838
- OMA:   AYNGISE
- BioCyc:   MKAN190192:MK1206-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01032
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR02087

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 18714; Mature: 18714

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKN
CCCCCCCEEECCCCCCCCCCHHHHHHHCCHHHHCCEEECCCCCCHHHHHCCCCEEEECCC
FGCGSSREQAVMALQQAGVACVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVT
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEE
VDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKNPDKPPWRD
EEHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEHHHHCCCCCCCCCC
>Mature Secondary Structure
MRWSGRAHVFGDDVDTDQIIPGRCLRRVSYDELGRYAMTGADPEFPEKVREGDVIVAGKN
CCCCCCCEEECCCCCCCCCCHHHHHHHCCHHHHCCEEECCCCCCHHHHHCCCCEEEECCC
FGCGSSREQAVMALQQAGVACVVARSFARIFYRNAINRGLPTVEAEEDPTEVVEDGNRVT
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEE
VDLDELVLRAGSEEVPLREPPEFALQAWREGGLLELVKKNPDKPPWRD
EEHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEEHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014