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The map label for this gene is RadA_1 [H]

Identifier: 20094468

GI number: 20094468

Start: 992204

End: 993154

Strand: Direct

Name: RadA_1 [H]

Synonym: MK1032

Alternate gene names: 20094468

Gene position: 992204-993154 (Clockwise)

Preceding gene: 20094466

Following gene: 20094471

Centisome position: 58.54

GC content: 58.57

Gene sequence:

>951_bases
GTGGTCGAGGAAGACGTCGTCGAGATAACCTCCCGCTATCTCCGCAGGGATCCCGATAAAATCGCTGAGATCTACAAAGC
CATAGGTATCGAGACACTCTCGGACCTCGCTGCGACCGACCCTGCTACAATTTCGGACGCTTTCGGTGTGAAGGAGAGCA
CAGCCAAGAAGATAATCGCGGACGCCCGCGAGGAAGCCAGTAAGGGTATCATGGAGGCCAAGACCCTTGCGGACCTCCTA
GAGGAAGAGAAGAAGCGTGACGTAATCCCCACGGGTATACAGGGTTTCGACGAGCGGATGGGCGGTGGACTACCGACCGG
CGTTATCGTCGGTATGTACGGACCGCCAGGTGCCGGTAAGTCACAGTTCGCCACCCAGGTGGCCGCGCACGCCCTTAAGG
AGGGTGAGTCGGTGCTCTACATCGACACCGAGAACGCCTTCCGACCGCAGCGTCTCCTAGAGATCGGTGGGTTCAAGAAG
GACGAACTGAAAGAAGTTTCCGACAGGTTCGTGCTGCGGCGTATCATCGACGCCGCGGCGCTGAGACAGTACTTCGACGA
GAAGGAGGGCGAGTTCATCAGCGAAGCCTACGAACTGACACCGAAGGTCGTGGTCATCGATTCCATATCACAGCCGTTCC
GTCCGTACAGCGCCCGCGATAAGCTACCGGAGCGATCGCGTATGATTGCCCACATCTTGAACACACTGCTCAAGTACTGC
ACCGCTTACAACGCCCTCGGCATGGTCACCACTCACGTCCAGGCGAACCCGGACGCTTGGGGTAAGCGCTGGCAAGACGT
GGCTCCAACGGTGCTCAAGCACATCGCCACGTACCGGTTCAGCATCGACTACAAGGGTAGGACTGAGCGGGTGATCACGC
TCGAAGACGCTCCCGACAAGCCACCGTTCGAGGTCCAAGTGGAACTCACGGACCGCGGTTTAGTGGGCTAA

Upstream 100 bases:

>100_bases
GCTGAAACTTTGCCATCTCAAACCTATTTCATAACCGATTTTAATCGAAGATAAGCTTAATATCTCACCCGAAGCTTACC
GAAGTGCGGGGGGTGATAAC

Downstream 100 bases:

>100_bases
CTGCGCAACTGTGCGATCCCCGGATGAGGATACAGCCGGGACCCCGAGGACCGATGCGGAGTGTGGCCGTAGCTGACGGG
CCTTCACCTCCGCACACGGA

Product: RadA recombinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIADAREEASKGIMEAKTLADLL
EEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGKSQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKK
DELKEVSDRFVLRRIIDAAALRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYC
TAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDKPPFEVQVELTDRGLVG

Sequences:

>Translated_316_residues
MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIADAREEASKGIMEAKTLADLL
EEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGKSQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKK
DELKEVSDRFVLRRIIDAAALRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYC
TAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDKPPFEVQVELTDRGLVG
>Mature_316_residues
MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIADAREEASKGIMEAKTLADLL
EEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGKSQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKK
DELKEVSDRFVLRRIIDAAALRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYC
TAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDKPPFEVQVELTDRGLVG

Specific function: Involved in DNA repair and in homologous recombination. Binds and assemble on single-stranded DNA to form a nucleoprotein filament. Hydrolyzes ATP in a ssDNA-dependent manner and promotes DNA strand exchange between homologous DNA molecules [H]

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the eukaryotic recA-like protein family [H]

Homologues:

Organism=Homo sapiens, GI23238219, Length=317, Percent_Identity=29.3375394321767, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI19924133, Length=301, Percent_Identity=25.2491694352159, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI17402896, Length=331, Percent_Identity=23.2628398791541, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI256017143, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI256017141, Length=226, Percent_Identity=26.5486725663717, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI256017145, Length=220, Percent_Identity=26.3636363636364, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71997301, Length=302, Percent_Identity=26.158940397351, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71997295, Length=302, Percent_Identity=26.158940397351, Blast_Score=88, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321027, Length=306, Percent_Identity=27.4509803921569, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320942, Length=284, Percent_Identity=24.6478873239437, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24651285, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864108, Length=279, Percent_Identity=25.8064516129032, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013632
- InterPro:   IPR011938
- InterPro:   IPR016467
- InterPro:   IPR020588
- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR020587 [H]

Pfam domain/function: PF08423 Rad51 [H]

EC number: NA

Molecular weight: Translated: 35211; Mature: 35211

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHH
DAREEASKGIMEAKTLADLLEEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCH
SQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKKDELKEVSDRFVLRRIIDAAA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYC
HHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
TAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDK
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCC
PPFEVQVELTDRGLVG
CCCEEEEEECCCCCCC
>Mature Secondary Structure
MVEEDVVEITSRYLRRDPDKIAEIYKAIGIETLSDLAATDPATISDAFGVKESTAKKIIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHHHH
DAREEASKGIMEAKTLADLLEEEKKRDVIPTGIQGFDERMGGGLPTGVIVGMYGPPGAGK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCCH
SQFATQVAAHALKEGESVLYIDTENAFRPQRLLEIGGFKKDELKEVSDRFVLRRIIDAAA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LRQYFDEKEGEFISEAYELTPKVVVIDSISQPFRPYSARDKLPERSRMIAHILNTLLKYC
HHHHHHHHCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHH
TAYNALGMVTTHVQANPDAWGKRWQDVAPTVLKHIATYRFSIDYKGRTERVITLEDAPDK
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEECCCCCC
PPFEVQVELTDRGLVG
CCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA