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The map label for this gene is DnaN

Identifier: 20094466

GI number: 20094466

Start: 990746

End: 991507

Strand: Direct

Name: DnaN

Synonym: MK1030

Alternate gene names: 20094466

Gene position: 990746-991507 (Clockwise)

Preceding gene: 20094465

Following gene: 20094468

Centisome position: 58.45

GC content: 59.58

Gene sequence:

>762_bases
TTGGTGGAGTTCAGGGCCTACCAGGAGGAGGCTAGGTACTTCAAGTACGCGTTCAACGCGGCGGGCAAGGTGGTCGAAGA
GGCCCCCTTGATCGTGACGGAGAACGGGATCGTGTCCAGGGCGATGGATGCCTCGCATATCGCTATGGCAGTGCTGGAGA
TGCCCTGGGAGATGTTCGACGAGTACGAGCCCCCGAGCGACGAGCTGATGTACGGTCTCGACATGGAGGAGGTGACCCGG
ATAGTGCGGCGTGCGCGGGTTACGGACGAGATCACCCTGGAGGGTGAGGACGAGGAGGAGGTGATAATCAAACTCGGATC
CAGCGGCTACGAGCGTGAGTTCAGGCTCCGGAGTATCGACATCGATGACATCCCCGACGAGCCCGAGCTGGATTTCGCGG
TCGAGGTTACCGTGGTGCCGGACTTCATCCAAGACGCCGTACGGGACGCGGATCTCGTCAGTGACACGGTCAAGGTCGGG
GCGAAGGGTAACACGTTCTACTTCAAGGCCGAAGGAGAGCGTGGTCGCGTGATTCCGAAGGTTCAGGAAGGTGCGGAAGC
GTTACTGACGTTCGAAGTCGAAGAGGATGTCGAGACCGCGTACCCGCTGGACTACCTGAAGGACATGATCCAGGCTGCTC
AAGGCGCGGAGAGTGTTAGGATCAGACTAGGTCAGGACATGCCTTTGGAGCTGACATTCAGGATCGGTCCCGCAGGTGAA
GGTAAGTTGACGTTCTACCTCGCGCCGCGAGTGGAGGAGTGA

Upstream 100 bases:

>100_bases
GTTGGGGATAGACCCGGAGTTGTGGAAGGACGTCCGGGAGGCGCTGGGGTAAGGGTTTATCTTTCACACCCTCGCGCCAG
AGTGCATCGGGGTGCTTAAG

Downstream 100 bases:

>100_bases
ACCCTTCCTCCTTCTCGCCAGAATCCCGCATTATCCGGGACAGGACCGTGGCTGGGTGGGGGACTAGCCCCCGTACAAGT
ACCTGTCGAGGAGCGCTTTC

Product: DNA polymerase sliding clamp (PCNA)

Products: NA

Alternate protein names: Proliferating cell nuclear antigen homolog; PCNA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFDEYEPPSDELMYGLDMEEVTR
IVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSIDIDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVG
AKGNTFYFKAEGERGRVIPKVQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGE
GKLTFYLAPRVEE

Sequences:

>Translated_253_residues
MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFDEYEPPSDELMYGLDMEEVTR
IVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSIDIDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVG
AKGNTFYFKAEGERGRVIPKVQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGE
GKLTFYLAPRVEE
>Mature_253_residues
MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFDEYEPPSDELMYGLDMEEVTR
IVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSIDIDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVG
AKGNTFYFKAEGERGRVIPKVQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGE
GKLTFYLAPRVEE

Specific function: Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PCNA family

Homologues:

Organism=Homo sapiens, GI33239451, Length=260, Percent_Identity=25, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI4505641, Length=260, Percent_Identity=25, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71995072, Length=227, Percent_Identity=24.6696035242291, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319564, Length=254, Percent_Identity=27.5590551181102, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585303, Length=258, Percent_Identity=23.2558139534884, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136790, Length=260, Percent_Identity=25, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45552759, Length=260, Percent_Identity=25, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCNA_METKA (Q8TWK3)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614313.1
- ProteinModelPortal:   Q8TWK3
- GeneID:   1477131
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1030
- NMPDR:   fig|190192.1.peg.1026
- HOGENOM:   HBG553564
- OMA:   STIRAEP
- BioCyc:   MKAN190192:MK1030-MONOMER
- HAMAP:   MF_00317
- InterPro:   IPR000730
- InterPro:   IPR022649
- InterPro:   IPR022659
- InterPro:   IPR022648
- PANTHER:   PTHR11352
- PRINTS:   PR00339
- TIGRFAMs:   TIGR00590

Pfam domain/function: PF02747 PCNA_C; PF00705 PCNA_N

EC number: NA

Molecular weight: Translated: 28626; Mature: 28626

Theoretical pI: Translated: 3.99; Mature: 3.99

Prosite motif: PS01251 PCNA_1; PS00293 PCNA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFD
CCCCHHHHHHHHHHHHHHHHHCHHHHCCCEEEECCCCHHHHCCHHHHHHHHHHCCHHHHH
EYEPPSDELMYGLDMEEVTRIVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSID
CCCCCHHHEEECCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCEEEEEEEC
IDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVGAKGNTFYFKAEGERGRVIPK
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCH
VQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGE
HHCCCEEEEEEEECCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCC
GKLTFYLAPRVEE
CEEEEEECCCCCC
>Mature Secondary Structure
MVEFRAYQEEARYFKYAFNAAGKVVEEAPLIVTENGIVSRAMDASHIAMAVLEMPWEMFD
CCCCHHHHHHHHHHHHHHHHHCHHHHCCCEEEECCCCHHHHCCHHHHHHHHHHCCHHHHH
EYEPPSDELMYGLDMEEVTRIVRRARVTDEITLEGEDEEEVIIKLGSSGYEREFRLRSID
CCCCCHHHEEECCCHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCCCCEEEEEEEC
IDDIPDEPELDFAVEVTVVPDFIQDAVRDADLVSDTVKVGAKGNTFYFKAEGERGRVIPK
CCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCH
VQEGAEALLTFEVEEDVETAYPLDYLKDMIQAAQGAESVRIRLGQDMPLELTFRIGPAGE
HHCCCEEEEEEEECCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCC
GKLTFYLAPRVEE
CEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014