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The map label for this gene is NtpA

Identifier: 20094453

GI number: 20094453

Start: 979665

End: 981443

Strand: Direct

Name: NtpA

Synonym: MK1017

Alternate gene names: 20094453

Gene position: 979665-981443 (Clockwise)

Preceding gene: 20094452

Following gene: 20094454

Centisome position: 57.8

GC content: 61.83

Gene sequence:

>1779_bases
GTGTCCAACAGCGTGAAAGGCGAGATAGTGAAGATCGCGGGACCCGTCGTGGAGGCGGTGGGTTGTGAGGGCGCGAAGAT
GTACGAGGTGTTCCGGGTCGGCGACGAGGGTCTAATCGGTGAGGTCATCAACATCGAGAGCGACCGGGCTACTATACAGG
TCTACGAAGAAACTACCGGTCTGCAGCCCGGTGAGCCCGTCAAGGGTACCGGAGAGCTCCTATCCGTGGAGTTAGGTCCC
GGACTGCTTACTCAGATCTTCGACGGAATCCAGCGCCCGCTGCCCGAGATCCGTAAGGAAGTCGGTGACTTCGTCGAGCG
TGGTATCCTCGTGTCGGCCCTGGACCGGAAGAAGAAGTGGGAGTTCACTCCCAAGGTCAAGGAGGGCGAGAAGGTCGAAG
AGGGCGACGTCCTCGGAACCGTGCCGGAGACGGAGTTCATCGAGCACAAGATCATGGTTCCGCCCGGTGTGTCCGGTGAG
GTAATCGAGATCGCGGCCGACGGAGAGTACACCGTGGAGGACACCATCGCCGTCATCGAGGACGAAGAGGGCGAGGAGCA
CGAGGTCACCATGATGCAGGAGTGGCCCGTGCGGAAGCCGCGACCTTACAAGCGCAAGCTCGACCCGGAGGAGCCCCTGA
TCACGGGTCAGCGTGTCATTGACACGTTCTTCCCCGTGGCCAAGGGTGGAACCGCCGCTATTCCGGGGCCGTTCGGGAGC
GGTAAGACGGTTACCCAGCAGCAGCTGGCGAAGTGGGCGGACGCCCAGGTAGTGGTGTACATCGGATGCGGAGAGCGTGG
TAACGAGATGACCGAGGTGCTCGAGGACTTCCCGGAACTCGAGGACCCACGGACGGGTAGGCCCCTGATGGAGCGTACGA
TCCTGGTAGCGAACACGTCCAACATGCCGGTGGCGGCCCGTGAAGCCTGTATCTACACCGGTATCACGATGGCGGAGTAC
TACCGGGACATGGGCTACGACGTGGCGCTCATGGCCGACTCCACGTCGCGGTGGGCCGAGGCGCTGCGTGAGATCTCAGG
TCGACTGGAGGAGATGCCGGGTGAGGAGGGTTACCCGGCGTACCTGGCCTCCAGACTCGCCGAGTTCTACGAGCGTGCGG
GTCGCGTCGTCTGTCTGGGTTCCGACGACCGAGTAGGTTCGGTCACCGTGGTGGGTGCGGTGTCCCCACCGGGTGGTGAC
TTCTCAGAGCCCGTAACGCAGAACACACTACGTATCGTGAAGGTCTTCTGGGCGCTCGACTCCAAGCTGGCGGACCGTCG
GCACTTCCCTGCGATCAACTGGTTGCAGTCGTACTCGCTGTACCTGGACGACGTCGAGAAGTGGTGGCACGAGGAGATCG
GTGGTGACTGGAGGGAGCTACGCGACGAGGCTATGGAGATACTCCAGCGCGAGTCGGAGCTGGAGGAGATCGTGCAGCTG
GTGGGACCGGACGCGCTGCCGGAGAGCGAGCGTCTGATCCTAGAGGTCGCGAGAATGATCCGTGAGGACTTCCTGCAACA
GAACGCGTTCCACGAGGTCGACACGTACTGCCCGCCCGAGAAGCAGTACGAGATGCTCAAGACCATCCTGCACTTCAAGG
AGAGGGCCGAGGAGGCCGTGGACAAGGGAGTGCCGGTGGACGAGATCCTCAAGCTCGACGTCATCGACGACATCGCCAGG
ATGAAGGTGATACCCAACGAGGAAGCGAAGGAGAAGATACAGGAGATCCGGAAGAAGATCGACGAGCAGTTCGAGGAGCT
CATAGAGGAGGCCTCGTAA

Upstream 100 bases:

>100_bases
CGGAAGGCCCAACCGAGGAGTTCGAGATCGAGGAGATAGTTAAAAAGGCGGTAGGAGTGGAGATCGACCTCGAACGCATC
GAGCGTTAGGGGGATCTTCC

Downstream 100 bases:

>100_bases
ATCCCCCTTCTCTCCCTCGGATCGTTACGGGGGTCGTAACGTGAGAGAGTTCCTCGTACTGTTCAACCACGCCCCTACGT
CACCCGACCGCGTGCGGCTC

Product: V-type ATP synthase subunit A

Products: NA

Alternate protein names: V-ATPase subunit A

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MSNSVKGEIVKIAGPVVEAVGCEGAKMYEVFRVGDEGLIGEVINIESDRATIQVYEETTGLQPGEPVKGTGELLSVELGP
GLLTQIFDGIQRPLPEIRKEVGDFVERGILVSALDRKKKWEFTPKVKEGEKVEEGDVLGTVPETEFIEHKIMVPPGVSGE
VIEIAADGEYTVEDTIAVIEDEEGEEHEVTMMQEWPVRKPRPYKRKLDPEEPLITGQRVIDTFFPVAKGGTAAIPGPFGS
GKTVTQQQLAKWADAQVVVYIGCGERGNEMTEVLEDFPELEDPRTGRPLMERTILVANTSNMPVAAREACIYTGITMAEY
YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFYERAGRVVCLGSDDRVGSVTVVGAVSPPGGD
FSEPVTQNTLRIVKVFWALDSKLADRRHFPAINWLQSYSLYLDDVEKWWHEEIGGDWRELRDEAMEILQRESELEEIVQL
VGPDALPESERLILEVARMIREDFLQQNAFHEVDTYCPPEKQYEMLKTILHFKERAEEAVDKGVPVDEILKLDVIDDIAR
MKVIPNEEAKEKIQEIRKKIDEQFEELIEEAS

Sequences:

>Translated_592_residues
MSNSVKGEIVKIAGPVVEAVGCEGAKMYEVFRVGDEGLIGEVINIESDRATIQVYEETTGLQPGEPVKGTGELLSVELGP
GLLTQIFDGIQRPLPEIRKEVGDFVERGILVSALDRKKKWEFTPKVKEGEKVEEGDVLGTVPETEFIEHKIMVPPGVSGE
VIEIAADGEYTVEDTIAVIEDEEGEEHEVTMMQEWPVRKPRPYKRKLDPEEPLITGQRVIDTFFPVAKGGTAAIPGPFGS
GKTVTQQQLAKWADAQVVVYIGCGERGNEMTEVLEDFPELEDPRTGRPLMERTILVANTSNMPVAAREACIYTGITMAEY
YRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFYERAGRVVCLGSDDRVGSVTVVGAVSPPGGD
FSEPVTQNTLRIVKVFWALDSKLADRRHFPAINWLQSYSLYLDDVEKWWHEEIGGDWRELRDEAMEILQRESELEEIVQL
VGPDALPESERLILEVARMIREDFLQQNAFHEVDTYCPPEKQYEMLKTILHFKERAEEAVDKGVPVDEILKLDVIDDIAR
MKVIPNEEAKEKIQEIRKKIDEQFEELIEEAS
>Mature_591_residues
SNSVKGEIVKIAGPVVEAVGCEGAKMYEVFRVGDEGLIGEVINIESDRATIQVYEETTGLQPGEPVKGTGELLSVELGPG
LLTQIFDGIQRPLPEIRKEVGDFVERGILVSALDRKKKWEFTPKVKEGEKVEEGDVLGTVPETEFIEHKIMVPPGVSGEV
IEIAADGEYTVEDTIAVIEDEEGEEHEVTMMQEWPVRKPRPYKRKLDPEEPLITGQRVIDTFFPVAKGGTAAIPGPFGSG
KTVTQQQLAKWADAQVVVYIGCGERGNEMTEVLEDFPELEDPRTGRPLMERTILVANTSNMPVAAREACIYTGITMAEYY
RDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPAYLASRLAEFYERAGRVVCLGSDDRVGSVTVVGAVSPPGGDF
SEPVTQNTLRIVKVFWALDSKLADRRHFPAINWLQSYSLYLDDVEKWWHEEIGGDWRELRDEAMEILQRESELEEIVQLV
GPDALPESERLILEVARMIREDFLQQNAFHEVDTYCPPEKQYEMLKTILHFKERAEEAVDKGVPVDEILKLDVIDDIARM
KVIPNEEAKEKIQEIRKKIDEQFEELIEEAS

Specific function: Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit

COG id: COG1155

COG function: function code C; Archaeal/vacuolar-type H+-ATPase subunit A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATPase alpha/beta chains family

Homologues:

Organism=Homo sapiens, GI19913424, Length=596, Percent_Identity=52.0134228187919, Blast_Score=615, Evalue=1e-176,
Organism=Homo sapiens, GI32189394, Length=360, Percent_Identity=29.4444444444444, Blast_Score=119, Evalue=1e-26,
Organism=Homo sapiens, GI19913428, Length=326, Percent_Identity=26.0736196319018, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI19913426, Length=331, Percent_Identity=25.9818731117825, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI50345984, Length=267, Percent_Identity=25.0936329588015, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI4757810, Length=267, Percent_Identity=25.0936329588015, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1790170, Length=483, Percent_Identity=26.9151138716356, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1790172, Length=237, Percent_Identity=28.2700421940928, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788251, Length=306, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17565854, Length=599, Percent_Identity=52.0868113522538, Blast_Score=615, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI25144756, Length=304, Percent_Identity=30.2631578947368, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17510931, Length=324, Percent_Identity=27.1604938271605, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17570191, Length=343, Percent_Identity=25.6559766763848, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988080, Length=267, Percent_Identity=25.8426966292135, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71988063, Length=267, Percent_Identity=25.8426966292135, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71988074, Length=267, Percent_Identity=25.8426966292135, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320016, Length=305, Percent_Identity=54.7540983606557, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6322581, Length=366, Percent_Identity=30.0546448087432, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319603, Length=344, Percent_Identity=26.453488372093, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6319370, Length=237, Percent_Identity=25.7383966244726, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583988, Length=594, Percent_Identity=51.8518518518518, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24583986, Length=594, Percent_Identity=51.8518518518518, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24583984, Length=594, Percent_Identity=51.8518518518518, Blast_Score=607, Evalue=1e-174,
Organism=Drosophila melanogaster, GI24583992, Length=591, Percent_Identity=51.6074450084602, Blast_Score=602, Evalue=1e-172,
Organism=Drosophila melanogaster, GI20129479, Length=536, Percent_Identity=55.2238805970149, Blast_Score=596, Evalue=1e-170,
Organism=Drosophila melanogaster, GI28574560, Length=336, Percent_Identity=30.3571428571429, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24638766, Length=307, Percent_Identity=30.6188925081433, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281361666, Length=332, Percent_Identity=26.2048192771084, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24646341, Length=332, Percent_Identity=26.2048192771084, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136796, Length=332, Percent_Identity=26.2048192771084, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24658560, Length=267, Percent_Identity=24.7191011235955, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: 10836 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): VATA_METKA (Q8TWL6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614300.1
- ProteinModelPortal:   Q8TWL6
- SMR:   Q8TWL6
- GeneID:   1477118
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1017
- NMPDR:   fig|190192.1.peg.1013
- HOGENOM:   HBG288114
- OMA:   KITWNVI
- ProtClustDB:   PRK04192
- BioCyc:   MKAN190192:MK1017-MONOMER
- BRENDA:   3.6.3.14
- HAMAP:   MF_00309
- InterPro:   IPR020003
- InterPro:   IPR000194
- InterPro:   IPR005726
- InterPro:   IPR003593
- InterPro:   IPR018118
- InterPro:   IPR000793
- InterPro:   IPR004100
- InterPro:   IPR022878
- SMART:   SM00382
- TIGRFAMs:   TIGR01043

Pfam domain/function: PF00006 ATP-synt_ab; PF00306 ATP-synt_ab_C; PF02874 ATP-synt_ab_N; SSF47917 ATPase_a/b_C; SSF50615 ATPase_a/b_N

EC number: =3.6.3.14

Molecular weight: Translated: 66495; Mature: 66364

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS00152 ATPASE_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSVKGEIVKIAGPVVEAVGCEGAKMYEVFRVGDEGLIGEVINIESDRATIQVYEETTG
CCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHEECCCCCCEEEEEEHHCC
LQPGEPVKGTGELLSVELGPGLLTQIFDGIQRPLPEIRKEVGDFVERGILVSALDRKKKW
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCC
EFTPKVKEGEKVEEGDVLGTVPETEFIEHKIMVPPGVSGEVIEIAADGEYTVEDTIAVIE
CCCCCCCCCCCCCCCCEEECCCCHHHHCCEEECCCCCCCCEEEEECCCCEECCCEEEEEE
DEEGEEHEVTMMQEWPVRKPRPYKRKLDPEEPLITGQRVIDTFFPVAKGGTAAIPGPFGS
CCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GKTVTQQQLAKWADAQVVVYIGCGERGNEMTEVLEDFPELEDPRTGRPLMERTILVANTS
CCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECC
NMPVAAREACIYTGITMAEYYRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPA
CCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHH
YLASRLAEFYERAGRVVCLGSDDRVGSVTVVGAVSPPGGDFSEPVTQNTLRIVKVFWALD
HHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
SKLADRRHFPAINWLQSYSLYLDDVEKWWHEEIGGDWRELRDEAMEILQRESELEEIVQL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGPDALPESERLILEVARMIREDFLQQNAFHEVDTYCPPEKQYEMLKTILHFKERAEEAV
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DKGVPVDEILKLDVIDDIARMKVIPNEEAKEKIQEIRKKIDEQFEELIEEAS
HCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNSVKGEIVKIAGPVVEAVGCEGAKMYEVFRVGDEGLIGEVINIESDRATIQVYEETTG
CCCCCCCEEEEHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHEECCCCCCEEEEEEHHCC
LQPGEPVKGTGELLSVELGPGLLTQIFDGIQRPLPEIRKEVGDFVERGILVSALDRKKKW
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHCCCCC
EFTPKVKEGEKVEEGDVLGTVPETEFIEHKIMVPPGVSGEVIEIAADGEYTVEDTIAVIE
CCCCCCCCCCCCCCCCEEECCCCHHHHCCEEECCCCCCCCEEEEECCCCEECCCEEEEEE
DEEGEEHEVTMMQEWPVRKPRPYKRKLDPEEPLITGQRVIDTFFPVAKGGTAAIPGPFGS
CCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
GKTVTQQQLAKWADAQVVVYIGCGERGNEMTEVLEDFPELEDPRTGRPLMERTILVANTS
CCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCHHHCEEEEEECC
NMPVAAREACIYTGITMAEYYRDMGYDVALMADSTSRWAEALREISGRLEEMPGEEGYPA
CCCCHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHH
YLASRLAEFYERAGRVVCLGSDDRVGSVTVVGAVSPPGGDFSEPVTQNTLRIVKVFWALD
HHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
SKLADRRHFPAINWLQSYSLYLDDVEKWWHEEIGGDWRELRDEAMEILQRESELEEIVQL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGPDALPESERLILEVARMIREDFLQQNAFHEVDTYCPPEKQYEMLKTILHFKERAEEAV
HCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
DKGVPVDEILKLDVIDDIARMKVIPNEEAKEKIQEIRKKIDEQFEELIEEAS
HCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014