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The map label for this gene is MurD_1 [H]

Identifier: 20094431

GI number: 20094431

Start: 954953

End: 956260

Strand: Direct

Name: MurD_1 [H]

Synonym: MK0995

Alternate gene names: 20094431

Gene position: 954953-956260 (Clockwise)

Preceding gene: 20094430

Following gene: 20094432

Centisome position: 56.34

GC content: 59.71

Gene sequence:

>1308_bases
ATGAGCGTGGATGAGCTTCCATTCGACAGCGTTGCCGTCATCGGGCTGGGAGTGGAGGGACGTACCTGCGCGGCGAGGTT
GGCGGAACTGGGACTCAAGGTCTACGCCTCCGACATCAGGGAAGACGTCGACGTCTCATCGCTCGAGAGGCTTCCCAACG
TCGAGGTAGAGCTCGGTCATCACGACATCGACAGAATCCGCGAGTGCGACACCGTGTATTTCTCGCCCAGCATACCGAAC
GACGCGGAGATAGTCCGCGAAGTCCGCAACATCGGGGTTCCACCACTTGAGGATCTCCTCACATGGCCAGATTCTTCGAA
ATTCGTCGTGGTCAGCGGAACCAACGGTAAAACCACGACGGTTCATATGATCGATCACCTACTCGACAAACTCGGTATCG
ATCGAAAGATCGGTGGGAACGCCGGAGGGGGTTTCGACGGGTACGCTACCCTCTACGTGCGAGCCGAGTTGGCGCGTCCG
GAGGTCGTAGTCTGTGAGGTGTGTGACATGACGCTGGACTATTTCTCCGACCGAGCTCCACGGTACGATGTCGCGGTCTT
CCTGAACCTGGGTTTGGATCACCTGGACTACCACGGTTCGATCGAGAGGTATGCGGCCCGAGCAGCCCGTTTCTGCGATC
GGGCCCGAGAGACGGCGATCGTCAAGTGTGACGGGGAGGAACGGAAGGTAGTCGAAAGGATGGAGAGTACTCCCAGGTGT
TATGATGAGTTGGACGTGGAAGTCGATATACCGCTCCGAGGGGAGTTCTACAGGCTGGATGCCAAGGCCGCGCTGCTGGC
CGTCGCCGAGATAACCAGCGAACCTCCCGAGGAGCTGGCCCCGTTACTCTCGGACTTCACCAGTGTTCCCGGACGCATTA
AGGAGTACAAGCTAGGTGAAGGCCGGCTGGTCATCGGAAAGACCGACAACCTCTCCGCCCTGAAGGTCGTCTTAAGGGAC
TACGGACCCCTGGACGTCGTCTTCTGGGGGACTCCGAGGCCGGGCGAAGATTACCGAATCGAGGGAATCGGCCGGACCCT
CCGGGAGGCGGGGGTAGAACGCGTCTACGTGTTTCCCGGCCTGTCGGAGGAGACCGTGGACGACGTGATCGAGGAGCTCA
CGAGCTCCGGAATTGAGGCGTCTCCGGTAGAGCCTTCCGAGGTCGCCTCGAAGGCTCTGGAGCTGGCGCGGCGTGGTCGG
GATGTGGGAGTGCTAGGAAACGGGCAGGATGTTATAATCTCAATACAACATGACGTCGAGCGTGCGGTACGCTTTTTGAC
CGCTGGATCGGAGCGATCTCGGGGATGA

Upstream 100 bases:

>100_bases
GCGAAGCTGGAGGAAATACTGACGCGCCTCGACCGTCTTGAAGCGAAGATCGACGAGGCTCTCGCGCGTATCGAAAGGCT
GGAACGGGAGCTGAGGGAGG

Downstream 100 bases:

>100_bases
GCCGGGATGGAGGTTGAATTCATACGCTTGGCGGACGAGATCCCGGAGTGTGAGTTCTTGATTGAGGGATACCCAGGTAT
AGGGATGGTGGGCGTAATCG

Product: UDP-N-acetylmuramoylalanine-D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGHHDIDRIRECDTVYFSPSIPN
DAEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTTVHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARP
EVVVCEVCDMTLDYFSDRAPRYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRC
YDELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGEGRLVIGKTDNLSALKVVLRD
YGPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPGLSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGR
DVGVLGNGQDVIISIQHDVERAVRFLTAGSERSRG

Sequences:

>Translated_435_residues
MSVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGHHDIDRIRECDTVYFSPSIPN
DAEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTTVHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARP
EVVVCEVCDMTLDYFSDRAPRYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRC
YDELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGEGRLVIGKTDNLSALKVVLRD
YGPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPGLSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGR
DVGVLGNGQDVIISIQHDVERAVRFLTAGSERSRG
>Mature_434_residues
SVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGHHDIDRIRECDTVYFSPSIPND
AEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTTVHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARPE
VVVCEVCDMTLDYFSDRAPRYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRCY
DELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGEGRLVIGKTDNLSALKVVLRDY
GPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPGLSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGRD
VGVLGNGQDVIISIQHDVERAVRFLTAGSERSRG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47953; Mature: 47821

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGH
CCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECC
HDIDRIRECDTVYFSPSIPNDAEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTT
CHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEECCCCCEEH
VHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARPEVVVCEVCDMTLDYFSDRAP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHCCCC
RYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRC
CEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCC
YDELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGE
HHHCCCEEECCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCC
GRLVIGKTDNLSALKVVLRDYGPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPG
CEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCCEEEEECC
LSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGRDVGVLGNGQDVIISIQHDVE
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHHH
RAVRFLTAGSERSRG
HHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVDELPFDSVAVIGLGVEGRTCAARLAELGLKVYASDIREDVDVSSLERLPNVEVELGH
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEECC
HDIDRIRECDTVYFSPSIPNDAEIVREVRNIGVPPLEDLLTWPDSSKFVVVSGTNGKTTT
CHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCEEEEECCCCCEEH
VHMIDHLLDKLGIDRKIGGNAGGGFDGYATLYVRAELARPEVVVCEVCDMTLDYFSDRAP
HHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHHCCCC
RYDVAVFLNLGLDHLDYHGSIERYAARAARFCDRARETAIVKCDGEERKVVERMESTPRC
CEEEEEEEECCCHHHCCCCCHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCC
YDELDVEVDIPLRGEFYRLDAKAALLAVAEITSEPPEELAPLLSDFTSVPGRIKEYKLGE
HHHCCCEEECCCCCCEEEECHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEECCC
GRLVIGKTDNLSALKVVLRDYGPLDVVFWGTPRPGEDYRIEGIGRTLREAGVERVYVFPG
CEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEHHHHHHHHHCCCCEEEEECC
LSEETVDDVIEELTSSGIEASPVEPSEVASKALELARRGRDVGVLGNGQDVIISIQHDVE
CCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEHHHHH
RAVRFLTAGSERSRG
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA