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The map label for this gene is pqqE [H]

Identifier: 20094416

GI number: 20094416

Start: 941177

End: 942388

Strand: Reverse

Name: pqqE [H]

Synonym: MK0980

Alternate gene names: 20094416

Gene position: 942388-941177 (Counterclockwise)

Preceding gene: 20094417

Following gene: 20094411

Centisome position: 55.6

GC content: 60.48

Gene sequence:

>1212_bases
TTGACCGCTATTACGGTGCTCGAGAAGCCGGGGATTCGGGTCAAAGCCAGGGTGAAACAGGGCCGTGTTAGCTTCGAGAT
GGAAGGTAAGCTCGCTTCACTCCTGAGACCACTCAAGAGGTTGTTCGAACACCTAGAGGAGGAGAAGCCGGCGGCCGTCC
GCGAGGACAGGATCGTGTTCTCACTGTACTTACCACCGTTCCCCAGCCGGGCGTTCACGCGTCTCCTCCGGGCGAGGTTG
AAGTCCGAGGTCCTGGGCCGCCGCGTTCCAGAAGCCGTCACCATGGCCGTAACCCAGAGGTGTCCATGCAACTGCGTGCA
CTGCAGCGCCGACAGACGGAGACCGACGGAACTCTCGACGGAGGACTGGCATCGGGCGATTCGGGAAGCGCTCGACCTCG
GCACATACAACGTGACTTTCACCGGGGGAGACCCGCTCTTCAGAGAGGATTTACCCGAACTCATTCAAGCCGTGGACGAC
GACCGAGCTATTGCCACGGCGTTCACATCGGGGTACACCCTGAAAGACCGAGTGAAGGAGCTGAAAGAAGCCGGATTGTA
CGCAATACACGTCAGTATCGACAGTCCGGACCCCGAGGAGCACGACGAACTGAGAGGCGTCCCCGGACTCTTCGAGCGGT
GCATAAGCGGCATCAAAGCCGCCCTCGATGCGGGACTGCTAGTCGGAGTTTCGACTTATGCTACACCGGAAACCGTGGAG
ACTGGCAAGGTGGAGGACGTGGTCCGTCTCGCCGCCGACCTGGGAGCGCACGAGGTTACGATCTTCGACGCCGTACCGAC
CGGCCGGCTGCTTCACGAGGAGAGTGTGATACTATCGGACGAGCACCGAGAAGATCTCATTGACCTCCATCTCAGGTGGA
ACCGCGAGAAGAGTAGTGGACCACGGGTCTCCGCGATGTCTTACGTGAACAGCGAGCACGGAGCCGGGTGCTTCGCCGGG
TACGTGCAGTGCCACGTGACTAACGACGGCGAGGTCACACCGTGCGACTTCACCCCGATCTCCTTCGGAAATATCCGCGA
GGATGGACTAAAAGCGGCGTGGAAGCGAATGACTTCACATCCGGAGTGGGGCAAGTGGAGACCCCACTGCAGGATGCAGG
ATCCGGAGGTCAGGCGGAGGTACATCCGCAAGATTCCCCCCGACGCTACGTTGCCGGTCCGCATTGACGAACTCGAAGGG
GACGGGAGTTGA

Upstream 100 bases:

>100_bases
TTTAGCCTCCCGTCTTTCAACCACGGTCTCCATAGTCGCACTGATCCTCCTCGTACTCAACTTAAGAGCACCTTATCACG
TGTTGGGGACGTAACAGCCT

Downstream 100 bases:

>100_bases
AGGCGTACACAGCGCCTTTGGGCGAGGTCCTTCGGGACCTAGATCCGAAGATCGCACGGCGCCTTGAGAGGTTCCTCGAG
GATCTGACCGAGCGGTTAAG

Product: Fe-S oxidoreductase

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MTAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVFSLYLPPFPSRAFTRLLRARL
KSEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELSTEDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDD
DRAIATAFTSGYTLKDRVKELKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVE
TGKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSGPRVSAMSYVNSEHGAGCFAG
YVQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSHPEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEG
DGS

Sequences:

>Translated_403_residues
MTAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVFSLYLPPFPSRAFTRLLRARL
KSEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELSTEDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDD
DRAIATAFTSGYTLKDRVKELKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVE
TGKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSGPRVSAMSYVNSEHGAGCFAG
YVQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSHPEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEG
DGS
>Mature_402_residues
TAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVFSLYLPPFPSRAFTRLLRARLK
SEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELSTEDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDDD
RAIATAFTSGYTLKDRVKELKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVET
GKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSGPRVSAMSYVNSEHGAGCFAGY
VQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSHPEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEGD
GS

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Escherichia coli, GI1786999, Length=140, Percent_Identity=32.8571428571429, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 45241; Mature: 45110

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVF
CCEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
SLYLPPFPSRAFTRLLRARLKSEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELST
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
EDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDDDRAIATAFTSGYTLKDRVKE
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHHCCCCHHHHHHH
LKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVE
HHHCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCC
TGKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSG
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCEEECCCHHHHHEEEEEEECCCCCCC
PRVSAMSYVNSEHGAGCFAGYVQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSH
CCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEGDGS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TAITVLEKPGIRVKARVKQGRVSFEMEGKLASLLRPLKRLFEHLEEEKPAAVREDRIVF
CEEEEECCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
SLYLPPFPSRAFTRLLRARLKSEVLGRRVPEAVTMAVTQRCPCNCVHCSADRRRPTELST
EEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCH
EDWHRAIREALDLGTYNVTFTGGDPLFREDLPELIQAVDDDRAIATAFTSGYTLKDRVKE
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEHHHHCCCCHHHHHHH
LKEAGLYAIHVSIDSPDPEEHDELRGVPGLFERCISGIKAALDAGLLVGVSTYATPETVE
HHHCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHEEEECCCCCCCCC
TGKVEDVVRLAADLGAHEVTIFDAVPTGRLLHEESVILSDEHREDLIDLHLRWNREKSSG
CCCHHHHHHHHHHCCCCEEEEEECCCCCCCEECCCEEECCCHHHHHEEEEEEECCCCCCC
PRVSAMSYVNSEHGAGCFAGYVQCHVTNDGEVTPCDFTPISFGNIREDGLKAAWKRMTSH
CCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
PEWGKWRPHCRMQDPEVRRRYIRKIPPDATLPVRIDELEGDGS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]