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The map label for this gene is gatB

Identifier: 20094396

GI number: 20094396

Start: 921362

End: 922747

Strand: Direct

Name: gatB

Synonym: MK0960

Alternate gene names: 20094396

Gene position: 921362-922747 (Clockwise)

Preceding gene: 20094395

Following gene: 20094398

Centisome position: 54.36

GC content: 60.39

Gene sequence:

>1386_bases
GTGGCGGACGTGAAAATAGGATTCGAGGTCCACGTGCAACTCGACACCCGCACCAAACTCTTCTGTGACTGTCCCACGGA
CTACGAGGACGCGGAACCTAACGAGAACACGTGTCCCGTCTGCACCGGAATGCCCGGGGCGAAACCCCTCCCACCCAACG
AGGAAGCCCTCCTGATAGCCCTCGAAATCGCGCATATGCTGGATTGCGAGCCCGTTCTGGATCGCCCGTTGTACTTCCAG
CGGAAGCACTACGACTACCCTGACCTGCCCTCCGGGTACCAGCGTACCAGCGTGCCCATCGCGGTCAACGGCGAGCTCGA
CGGTGTTCGGATCCGCGAGATTCACGTCGAGGAGGATCCGGGCCGTTGGGAGCCCTCCACCGGGCGCGTCGATTACAACC
GGAGCGGCGTCCCGCTCATCGAGATCGTAACCGAGCCGGACATGCGTTCCCCGGAGGAGGCTCGAGACTTCCTGCGTCGT
CTCATGCAGGTCCTCCGGTACTCGGGTAAGGTGAAGGGTGACGGTGGCATCCGTGTAGATGCCAACGTCTCGGTCGAAGG
CGGGGCCAGGGTCGAGATCAAGAACATCAACTCGATCAAGGGCGTGTATCGGGCCCTCAGGTTCGAGATCCAGCGACAAC
TCAACTTGATGAAACATGGGCGCGAGGTCCGCCGCGAGACCCGTGCCTTCCGTGAGGATCAAGGTACCACGGTCGCGATG
CGTTCCAAGGAGACCGCGGAGGATTACAGGTACATCCCGGATCCGGACATACCGGTGTTCGAGATCACCGAAGACCTATG
GGAGAAGGCCGTCGCACGGGCTCCGGAGCCACCGCACCACCGTGCCAGGCGGATGGCGGAGGAGTACGGTATCTCGTTGG
AGGCCGCCGAGGCCCTGGTCACGGAGCGTGAGTGGGCCGATTTCTTCGAAGAGGTAGTGGAGAAGGCCCCGGATGACTGG
GACATCGAGTTCATCGACCAGTGGGTGCGTAAGGAGATCAAGAAGATACTCAACAAGAAGGAAATGACTTTCCGTGAAGC
TAAGATAACCCCGGAAGAGTTCATCGAGCTGCTCGAGCTCGTTCGTGAGGACAAGATCACACGCCAGAACGCTCTCAACG
CGCTGTGGGAAGCGGTCGATTCAGACAAGAGCCCCGTTGAGATCATCGAGGAGAACGGACTGCTCAAAGTTAGCGATGAG
GATCGGTTGGCGCGCGTCGTGGAGGAGGTCATCGAGGAGAACCCGCAGGCGGTGGAGGATTACAAATCGGGTAAGGAGGA
AGCCATCCACTACCTGATGGGCCAGGTGATGCGCAAAACCAGGGGACAGGCCGACCCGGAGGTCACCATGCGACTCCTCA
GGGAAAGGCTGGATTCCGACGGCTAA

Upstream 100 bases:

>100_bases
GGCGGCGCTGCTCAGGACGCTGTCACACGAGCTCGATCTGACGTCAGCCATCCGCTACTGATAAGTGCACGGCGGATCCG
TCAACTCCAGGGTTCACCAG

Downstream 100 bases:

>100_bases
CGGCGTCCCTCACATCGGAACACCACGGTCGGGGTGTCCGGGAAAGATTCCGCTCTCACCTCCAGAAAGAGCCGACTGAG
GGCTCGGTATATCCTATCCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIALEIAHMLDCEPVLDRPLYFQ
RKHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDPGRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRR
LMQVLRYSGKVKGDGGIRVDANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAM
RSKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALVTEREWADFFEEVVEKAPDDW
DIEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLELVREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDE
DRLARVVEEVIEENPQAVEDYKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG

Sequences:

>Translated_461_residues
MADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIALEIAHMLDCEPVLDRPLYFQ
RKHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDPGRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRR
LMQVLRYSGKVKGDGGIRVDANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAM
RSKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALVTEREWADFFEEVVEKAPDDW
DIEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLELVREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDE
DRLARVVEEVIEENPQAVEDYKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG
>Mature_460_residues
ADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIALEIAHMLDCEPVLDRPLYFQR
KHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDPGRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRRL
MQVLRYSGKVKGDGGIRVDANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAMR
SKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALVTEREWADFFEEVVEKAPDDWD
IEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLELVREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDED
RLARVVEEVIEENPQAVEDYKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=31.7269076305221, Blast_Score=232, Evalue=7e-61,
Organism=Caenorhabditis elegans, GI115534094, Length=472, Percent_Identity=32.4152542372881, Blast_Score=187, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6319391, Length=501, Percent_Identity=29.3413173652695, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI45550808, Length=492, Percent_Identity=28.6585365853659, Blast_Score=195, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_METKA (Q8TWS2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614243.1
- ProteinModelPortal:   Q8TWS2
- SMR:   Q8TWS2
- GeneID:   1477061
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0960
- NMPDR:   fig|190192.1.peg.956
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   MKAN190192:MK0960-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53160; Mature: 53028

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIA
CCCEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEH
LEIAHMLDCEPVLDRPLYFQRKHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDP
HHHHHHHCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCC
GRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRRLMQVLRYSGKVKGDGGIRVD
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
ANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAM
CEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
RSKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALV
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TEREWADFFEEVVEKAPDDWDIEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLEL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHH
VREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDEDRLARVVEEVIEENPQAVED
HHHHHHHHHHHHHHHHHHHCCCCCCHHHECCCCEEEECCHHHHHHHHHHHHHCCCHHHHH
YKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG
HHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADVKIGFEVHVQLDTRTKLFCDCPTDYEDAEPNENTCPVCTGMPGAKPLPPNEEALLIA
CCEEEEEEEEEEEECCCEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEH
LEIAHMLDCEPVLDRPLYFQRKHYDYPDLPSGYQRTSVPIAVNGELDGVRIREIHVEEDP
HHHHHHHCCCHHHCCCCHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCC
GRWEPSTGRVDYNRSGVPLIEIVTEPDMRSPEEARDFLRRLMQVLRYSGKVKGDGGIRVD
CCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEE
ANVSVEGGARVEIKNINSIKGVYRALRFEIQRQLNLMKHGREVRRETRAFREDQGTTVAM
CEEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
RSKETAEDYRYIPDPDIPVFEITEDLWEKAVARAPEPPHHRARRMAEEYGISLEAAEALV
CCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHH
TEREWADFFEEVVEKAPDDWDIEFIDQWVRKEIKKILNKKEMTFREAKITPEEFIELLEL
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHH
VREDKITRQNALNALWEAVDSDKSPVEIIEENGLLKVSDEDRLARVVEEVIEENPQAVED
HHHHHHHHHHHHHHHHHHHCCCCCCHHHECCCCEEEECCHHHHHHHHHHHHHCCCHHHHH
YKSGKEEAIHYLMGQVMRKTRGQADPEVTMRLLRERLDSDG
HHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014