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The map label for this gene is TrpB

Identifier: 20094221

GI number: 20094221

Start: 745708

End: 746904

Strand: Direct

Name: TrpB

Synonym: MK0784

Alternate gene names: 20094221

Gene position: 745708-746904 (Clockwise)

Preceding gene: 20094220

Following gene: 20094223

Centisome position: 44.0

GC content: 60.65

Gene sequence:

>1197_bases
GTGGATAGGTACCCGGACGAGAACGGGTATTTCGGTGAGTACGGAGGGCGTTTCGTGCCGGAGACGCTCATGCCCGCGCT
GGAGGAACTAGAGGATGCGTTCAAAGAAGCACGTGAAGACCCAGAGTTCTGGGAAGAACTCGAAGAACTGTGGCGGAAGT
ACGCCGGGCGCCCGACTCCACTGTACTACGCACGGAACCTGTCGCGGAAACTCGGCGTCAAGGTGTACCTCAAGCGCGAG
GACTTGGTCCACGGCGGGGCACACAAGCTGAACAACACACTCGGTCAGGCCCTGCTCGCCGATCGTATGGGTAAAGATCG
CATCATCGCGGAGACCGGTGCCGGACAACACGGACTCGCCACCGCCATGGCCGGGGCCGCACTGGGCAAGAAAGTCGAGA
TCTACATGGGGGCCATCGACGTAGAGCGCCAGAAACACAACGTCTTCAGGATGGAGCTGATGGGGGCCAAAGTCCACCCC
GTGAAAGCCGGAACGCAGACGCTCAAGGACGCGATCAACGAGGCGCTGCGCGACTGGATAACGAACCTCGAGACTACTCA
TTACCTCTTGGGTTCCGTCGTCGGGCCGCACCCTTACCCGTGGATCGTCCGCGAGTTTCAGCGGGTGATCGGCAGGGAGA
CGAAGGAGCAGATCACGGAGCTAGAGGGAGGTCTTCCGGACGCGATCGTGGCGTGTACGGGGGGAGGCAGTAACTCCATC
GGGATATTCTACGACTTCCTGGACGATGAGGAAGTAGCGCTATACGCCGTCGAGGCCGGTGGCAAGGGGCTGGATACTGA
CGAACATTCGGCCTCGCTGTGCGCCGGGGAGGTGGGGGTCCTCCACGGTTGTCGGACCAAAGTCCTTCAGGATGAGCACG
GCCAGATCCGACCGACACACAGCATCGCACCTGGTCTCGACTATCCTGGAGTGGGTCCGGAGCTCGCGTTCCTAGTGGAC
GAAGGTAGAGTCACCGCCGACGCCGTGACGGACGAGGAAGCACTGCGTGGTTTCGTCATGCTGAACGAAACCGAAGGGAT
ACTCCCAGCGCTCGAATCCGCACACGCGGTCTATTACGTGAAAAAGCTGGTGGAAAGAGGTGAACTGGACCGTGGGGACG
TTGTCGTCGTGAACCTGTCGGGGAGAGGCGACAAGGACGTAAGGATAGCGGCGGAGGAATTGGGGGTGGAGATCTAG

Upstream 100 bases:

>100_bases
TTTCGAGGAGGCCTGTAAGGTCATCACGGAAAGCGTCTGTGAAGAACGGTTCGAGGACGTCGTTTCGAGACTGTAGGGGA
GGCTTCGGTTGGGACGGTAC

Downstream 100 bases:

>100_bases
CTACTCCTTCGGTACTATACGGAACTTACAGATATCGTCGCCCGCCAGCTGGCACTTCGTCTCCTCTATCTCCATGCTCT
TATCGTACTTCAGCTCCGCT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTPLYYARNLSRKLGVKVYLKRE
DLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLATAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHP
VKAGTQTLKDAINEALRDWITNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSI
GIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTHSIAPGLDYPGVGPELAFLVD
EGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYVKKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI

Sequences:

>Translated_398_residues
MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTPLYYARNLSRKLGVKVYLKRE
DLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLATAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHP
VKAGTQTLKDAINEALRDWITNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSI
GIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTHSIAPGLDYPGVGPELAFLVD
EGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYVKKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI
>Mature_398_residues
MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTPLYYARNLSRKLGVKVYLKRE
DLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLATAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHP
VKAGTQTLKDAINEALRDWITNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSI
GIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTHSIAPGLDYPGVGPELAFLVD
EGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYVKKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=380, Percent_Identity=57.8947368421053, Blast_Score=449, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321412, Length=386, Percent_Identity=55.699481865285, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_METKA (Q8TX91)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614068.1
- ProteinModelPortal:   Q8TX91
- SMR:   Q8TX91
- GeneID:   1476885
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0784
- NMPDR:   fig|190192.1.peg.781
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   MKAN190192:MK0784-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43757; Mature: 43757

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
LYYARNLSRKLGVKVYLKREDLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLA
CHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
TAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHPVKAGTQTLKDAINEALRDWI
HHHHHHHCCCEEEEEEEEEEHHHHHCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHH
TNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSI
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCE
GIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTH
EEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIAPGLDYPGVGPELAFLVDEGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYV
CCCCCCCCCCCCCCEEEEEECCCEEEHHCCCHHHHCEEEEEECCCCCHHHHHHHHHHHHH
KKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI
HHHHHHCCCCCCCEEEEEECCCCCCCCEEEHHHCCCCC
>Mature Secondary Structure
MDRYPDENGYFGEYGGRFVPETLMPALEELEDAFKEAREDPEFWEELEELWRKYAGRPTP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
LYYARNLSRKLGVKVYLKREDLVHGGAHKLNNTLGQALLADRMGKDRIIAETGAGQHGLA
CHHHHHHHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
TAMAGAALGKKVEIYMGAIDVERQKHNVFRMELMGAKVHPVKAGTQTLKDAINEALRDWI
HHHHHHHCCCEEEEEEEEEEHHHHHCCEEEEEEECCEECCCCHHHHHHHHHHHHHHHHHH
TNLETTHYLLGSVVGPHPYPWIVREFQRVIGRETKEQITELEGGLPDAIVACTGGGSNSI
HCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCE
GIFYDFLDDEEVALYAVEAGGKGLDTDEHSASLCAGEVGVLHGCRTKVLQDEHGQIRPTH
EEEEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
SIAPGLDYPGVGPELAFLVDEGRVTADAVTDEEALRGFVMLNETEGILPALESAHAVYYV
CCCCCCCCCCCCCCEEEEEECCCEEEHHCCCHHHHCEEEEEECCCCCHHHHHHHHHHHHH
KKLVERGELDRGDVVVVNLSGRGDKDVRIAAEELGVEI
HHHHHHCCCCCCCEEEEEECCCCCCCCEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014