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The map label for this gene is rsmB [H]

Identifier: 20094206

GI number: 20094206

Start: 729717

End: 730787

Strand: Direct

Name: rsmB [H]

Synonym: MK0769

Alternate gene names: 20094206

Gene position: 729717-730787 (Clockwise)

Preceding gene: 20094205

Following gene: 20094207

Centisome position: 43.05

GC content: 63.03

Gene sequence:

>1071_bases
CTGGCCGCCCTCCGAACTCCGGTTCGGCGCTACACGCTCCGGGTTAACACTCTGAAAGCGGACCTGGAGGATGTCGTCGA
ATGTCTCGCCTCGGATTTTCCCGATCGGGAGGTTAGAGAGTCTCCTTACTCCGAGTACGCGGTCGAGATCGAGGTGAAAG
GACCGTACCCCGTGGAGGGGAACCACCACGTCGTAGTCGCCGACAAGTTCGCGGCCGAGAGCGTCTACGTGGGGGCGGAT
CTCTACGCTCCCGGGGTGGTGCAAGCGGACCCCGACATCCGTAGAGGCGACCGCGTGACCGTAGTCTCGGAACGCGGGCA
CCCGGTCGCATCCGGTGAGGCGGCGCTCCAGGGGCGTGAGATGGAAAAGCGGGACCGAGGTACTGCCGTCCGCGTGGATC
GCCCCACCTTTAGTGCCCCAAAAGTCCGTGAGACCGAAGCGTACCGGCGAGGTTGGGTGTACTCCCAGGGTCTGCCCTCC
ATCTTGGCCGTAGAGGCCCTGTCTCCGGAGCCCGGCGAAACCGTGGTCGACCTCTGTGCGGCTCCGGGTGGGAAGTGTTC
CCACGTGGCTCAGATCACCGGTCCGGAGTCCAAGATAGTAGCTATCGACCGCTCCGCACCACGACTCGAACGGATGGAGG
CTCGACTACGTCGCCTGGGTATCGACTGGGTCGAGACCGTGCACGGGGACGCCAGGAAGGTCGTCAGGAGGCTGCGGGGA
ACGGCAGACGTTGTCCTCGTTGACCCGCCTTGCACCGCCTTAGGTGTCAGGCCGAAACTCTGGGTCGAGGCGACTTACGA
GGAGGCGCTCGGGCTACCGTCTTACCAGTATTCGTTGCTCAGAGCCGGATACGAAGTCCTTAAGGAGGGCGGAAGACTCC
TATACTCCACGTGCACTCTCACGCCCACCGAAAACGAACTCGTGGTCGAACGTGCGATACGGGAGCTAAACCTTGAGCCC
GAGACGCCGGTGCTTCGACCCGCCAGACGCTCAGGTCCGGGCGTTGTCTTCCTCCCCCATCGTGCGGATGTCCCTGGATT
CTTCTACGCAGTACTCGTTAAGGAAGGATGA

Upstream 100 bases:

>100_bases
CCCGAGCTGAGGGGACACTCCCATCCTCCGAAAGACGGAGCTCGATGACCCGCGACTCGCGCACGTACTCCGTGAGCGCG
TAGCCGACTATCCCTCACTG

Downstream 100 bases:

>100_bases
GGGAGTAGACGTCGTGTCCGACGGCGTCGTGAAACGACGGGCAATACCGGCGATGGCGGCGGGTGTAGCCGTTGTCCTCA
TCATGATTTGGTCGTTCGAT

Product: tRNA/rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEGNHHVVVADKFAAESVYVGAD
LYAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGREMEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPS
ILAVEALSPEPGETVVDLCAAPGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRG
TADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTLTPTENELVVERAIRELNLEP
ETPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG

Sequences:

>Translated_356_residues
MAALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEGNHHVVVADKFAAESVYVGAD
LYAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGREMEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPS
ILAVEALSPEPGETVVDLCAAPGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRG
TADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTLTPTENELVVERAIRELNLEP
ETPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG
>Mature_355_residues
AALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEGNHHVVVADKFAAESVYVGADL
YAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGREMEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPSI
LAVEALSPEPGETVVDLCAAPGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRGT
ADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTLTPTENELVVERAIRELNLEPE
TPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI32698918, Length=374, Percent_Identity=26.2032085561497, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI76150625, Length=236, Percent_Identity=33.4745762711864, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI76150623, Length=236, Percent_Identity=33.4745762711864, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI270288818, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI270288816, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI23199998, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI8922322, Length=231, Percent_Identity=32.9004329004329, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI40316918, Length=175, Percent_Identity=37.1428571428571, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI39995082, Length=191, Percent_Identity=29.3193717277487, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI301336155, Length=186, Percent_Identity=29.5698924731183, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI11545785, Length=167, Percent_Identity=31.7365269461078, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367212, Length=248, Percent_Identity=32.6612903225806, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87081985, Length=238, Percent_Identity=29.8319327731092, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=236, Percent_Identity=28.8135593220339, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=194, Percent_Identity=29.8969072164948, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71998419, Length=193, Percent_Identity=30.0518134715026, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25143471, Length=188, Percent_Identity=30.8510638297872, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17509941, Length=169, Percent_Identity=32.5443786982249, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319447, Length=167, Percent_Identity=35.3293413173653, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=233, Percent_Identity=29.1845493562232, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24668781, Length=384, Percent_Identity=30.46875, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI21356513, Length=320, Percent_Identity=31.875, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI22024126, Length=236, Percent_Identity=31.7796610169492, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355201, Length=185, Percent_Identity=30.2702702702703, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356579, Length=173, Percent_Identity=30.0578034682081, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=31.9277108433735, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 39261; Mature: 39130

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEG
CCCHHCCHHHEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECCCCCCCC
NHHVVVADKFAAESVYVGADLYAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGRE
CEEEEEEECCCCCEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHHHCCCC
MEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPSILAVEALSPEPGETVVDLCA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCEECCCCCCEEEEEECCCCCCHHHHHHHC
APGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRG
CCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCC
TADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTL
CCCEEEECCCCHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEC
TPTENELVVERAIRELNLEPETPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
AALRTPVRRYTLRVNTLKADLEDVVECLASDFPDREVRESPYSEYAVEIEVKGPYPVEG
CCHHCCHHHEEEEEEHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEEEEECCCCCCCC
NHHVVVADKFAAESVYVGADLYAPGVVQADPDIRRGDRVTVVSERGHPVASGEAALQGRE
CEEEEEEECCCCCEEEECCCCCCCCEEECCCCCCCCCEEEEEECCCCCCCCCHHHHCCCC
MEKRDRGTAVRVDRPTFSAPKVRETEAYRRGWVYSQGLPSILAVEALSPEPGETVVDLCA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHCCEECCCCCCEEEEEECCCCCCHHHHHHHC
APGGKCSHVAQITGPESKIVAIDRSAPRLERMEARLRRLGIDWVETVHGDARKVVRRLRG
CCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHCC
TADVVLVDPPCTALGVRPKLWVEATYEEALGLPSYQYSLLRAGYEVLKEGGRLLYSTCTL
CCCEEEECCCCHHHCCCCEEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEC
TPTENELVVERAIRELNLEPETPVLRPARRSGPGVVFLPHRADVPGFFYAVLVKEG
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]