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The map label for this gene is 20094205

Identifier: 20094205

GI number: 20094205

Start: 729066

End: 729563

Strand: Direct

Name: 20094205

Synonym: MK0768

Alternate gene names: NA

Gene position: 729066-729563 (Clockwise)

Preceding gene: 20094198

Following gene: 20094206

Centisome position: 43.01

GC content: 60.64

Gene sequence:

>498_bases
ATGATGATCGGGATCTTGTCGGACACCCACGACAACCTGAAGGCCATCGAACGGCTTGCTAGCGAGCTGAACGAGGCGGA
CGTTGAGGTCGTACTCCATGCGGGTGACTACGTGGCTCCCTTCACCCTCCCGGTGCTCGCGAAGGTGGAGTGTGACGAGT
TTATAGGGGTTTTTGGAAACAACGACGGCGAGCGTGACTATTTGCGCGAAAAGGCGGAGGAAGTGGGCTTCGAGCTGGTC
GGCGAGATCTTCACGGGCGAGGTCTTGGGTCTCCGAGTGGCCATGATTCACGGCACCGAAGAGGCCGTAGTCGAGGCCCT
CGCCCGCTGTGGCGAGTACGATCTGGTGGTTTACGGCCACACCCACGAACCGGAGGAGCGCGTGGTCGGAGACACGCTCG
TGGTTAATCCCGGAGAGGTATGTGGGTACGTCACCGGGCGCCGAACGGCCGCGCTGCTCGACCCCGATGAGAAGAAGGTA
GAGTTCGTCGAGTTTTGA

Upstream 100 bases:

>100_bases
GCGAGACGCGAACGGCCTGCCGCAGCACGCGGCGAGAGTTCACCCCGGTGACGCGTTTCAAAACTTCGGGTAACCTTCCT
CACCCCCTCAAGGGGGATTC

Downstream 100 bases:

>100_bases
CCCGACATGCCCGATGAAGATCACGGCCTTCGCCTGAGACGTGACGAGATCCACCCGAGCTGAGGGGACACTCCCATCCT
CCGAAAGACGGAGCTCGATG

Product: phosphoesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGNNDGERDYLREKAEEVGFELV
GEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGHTHEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKV
EFVEF

Sequences:

>Translated_165_residues
MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGNNDGERDYLREKAEEVGFELV
GEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGHTHEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKV
EFVEF
>Mature_165_residues
MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGNNDGERDYLREKAEEVGFELV
GEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGHTHEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKV
EFVEF

Specific function: Shows phosphodiesterase activity, hydrolyzing phosphodiesters bonds in the artificial chromogenic substrates bis-p-nitrophenyl phosphate (bis-pNPP), and less efficiently thymidine 5'-monophosphate p-nitrophenyl ester (pNP-TMP) and p- nitrophenylphosphoryl

COG id: COG0622

COG function: function code R; Predicted phosphoesterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallophosphoesterase superfamily. YfcE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR020935
- InterPro:   IPR000979 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 18154; Mature: 18154

Theoretical pI: Translated: 4.07; Mature: 4.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGN
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHEEEECHHHHHEECCC
NDGERDYLREKAEEVGFELVGEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGH
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEECCHHHHHHHHHHCCCCEEEEEEC
THEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKVEFVEF
CCCCHHHHCCCEEEECCCHHEEEECCCCEEEEECCCCCCEEEECC
>Mature Secondary Structure
MMIGILSDTHDNLKAIERLASELNEADVEVVLHAGDYVAPFTLPVLAKVECDEFIGVFGN
CEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHEEEECHHHHHEECCC
NDGERDYLREKAEEVGFELVGEIFTGEVLGLRVAMIHGTEEAVVEALARCGEYDLVVYGH
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEECCHHHHHHHHHHCCCCEEEEEEC
THEPEERVVGDTLVVNPGEVCGYVTGRRTAALLDPDEKKVEFVEF
CCCCHHHHCCCEEEECCCHHEEEECCCCEEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]