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The map label for this gene is ppnK

Identifier: 20094179

GI number: 20094179

Start: 705044

End: 705874

Strand: Direct

Name: ppnK

Synonym: MK0742

Alternate gene names: 20094179

Gene position: 705044-705874 (Clockwise)

Preceding gene: 20094178

Following gene: 20094181

Centisome position: 41.6

GC content: 57.88

Gene sequence:

>831_bases
ATGTTCCGACCGCAGGTCGTCGGTGTGACCGGCAGAACGGACCTGGGTAGAGCAGTGCGGGTCGCGGAGAGAGTGTGTCG
TCTTTGCGACCGGGAAGGGTTCGAAGTCCTAGTGGACGATTCGCTGGGGATCGGCGAATACCCCCGGGTTAACTTGAAAG
ACATGGGCAAAGAGGTCGATATGATCATCACGATCGGCGGTGATGGGACCATACTCCGCGTCTCGAGGATTACGTCAGAG
TATGAGGTGCCGATCTTGGGTGTGAACCTAGGAAAGTTCGGATTCCTAACCGAGGTGAGTGAGAGTGGCCTGAAGGAAGC
CGTATCTCGGCTCGCTCGGGGAGATTTCAACCTAGAAGAACACCGAAAGCTCAGGATAAAGATAGGGGGATCCGACGAAG
GAGACGCTCTCAACGAGGTCACCGTGATCACGAGTCGACCTGCTAAAATGATCAGGTACCGGCTGTCCATCGACGGGTTC
GAGTTGGAGACCACGTGGGCGGACGGTGTACTAGTGGCGACCCCGACGGGTTCCACCGCCTACTCCCTCTCAGCCGGAGG
TCCAATCGTGGAGCCACAGGTGGAGTGTTCCATTATTACTCCTCTGAACCCGTTCAAGCTGGAGGCACGGCCTATGGTGG
TTTCCATGGACCGACGCGTGGAGATCGACGTGGACGACCCTGAGCGTGCCGAGGTCGTCGTCGACGGGCAGGAGTACATG
AATCTCGACGGCACGGTGTCCGTTACGCGATCCCCGAACGTGGCGCGGTTTATACGGTTCGGGTCGACGTACTTCGAGCG
GTTGAAGGAAAAGTTCCTGAGGTGGGATTGA

Upstream 100 bases:

>100_bases
AGGAGCTCGCGGACGTGTTCGCTCTCGTCATGAACCTAGCGAACGAGCTCGACGTGGACGTTTTGACGGAACTGCGGCGT
AAGTTTCGGAGGGTGTTCGG

Downstream 100 bases:

>100_bases
TGTCGCTACGCGACACGATCAAGGAAATCGGTGAGGCGATCTTCCGGGCGTTTCTAGTGGGACCACTGCCTAGGCTCCTA
GCGTGCGTGTGTTTCACCCT

Product: sugar kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVDMIITIGGDGTILRVSRITSE
YEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEEHRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGF
ELETTWADGVLVATPTGSTAYSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYM
NLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD

Sequences:

>Translated_276_residues
MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVDMIITIGGDGTILRVSRITSE
YEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEEHRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGF
ELETTWADGVLVATPTGSTAYSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYM
NLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD
>Mature_276_residues
MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVDMIITIGGDGTILRVSRITSE
YEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEEHRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGF
ELETTWADGVLVATPTGSTAYSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYM
NLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=266, Percent_Identity=28.9473684210526, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788968, Length=226, Percent_Identity=29.2035398230088, Blast_Score=124, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320794, Length=240, Percent_Identity=30.8333333333333, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322509, Length=218, Percent_Identity=29.8165137614679, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6325068, Length=178, Percent_Identity=33.1460674157303, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=28.0632411067194, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=28.0632411067194, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28573830, Length=213, Percent_Identity=28.6384976525822, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI28573832, Length=213, Percent_Identity=28.6384976525822, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI161077047, Length=213, Percent_Identity=28.6384976525822, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129957, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363323, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363321, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24653424, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24653422, Length=278, Percent_Identity=25.5395683453237, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_METKA (Q8TXD2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614026.1
- ProteinModelPortal:   Q8TXD2
- GeneID:   1476843
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0742
- NMPDR:   fig|190192.1.peg.739
- HOGENOM:   HBG713904
- OMA:   HGASGYV
- BioCyc:   MKAN190192:MK0742-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30665; Mature: 30665

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCCCEE
MIITIGGDGTILRVSRITSEYEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEE
EEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
HRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGFELETTWADGVLVATPTGSTA
CEEEEEEECCCCCCCCCCEEEEEECCCHHHEEEEEEECCEEEEEEECCCEEEECCCCCCE
YSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYM
EEECCCCCEECCCCEEEEECCCCCCEEECCCEEEEECCEEEEECCCCCCCEEEECCHHHC
NLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD
CCCCEEEEECCCCHHHHHHHCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFRPQVVGVTGRTDLGRAVRVAERVCRLCDREGFEVLVDDSLGIGEYPRVNLKDMGKEVD
CCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCCCEE
MIITIGGDGTILRVSRITSEYEVPILGVNLGKFGFLTEVSESGLKEAVSRLARGDFNLEE
EEEEECCCCCEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
HRKLRIKIGGSDEGDALNEVTVITSRPAKMIRYRLSIDGFELETTWADGVLVATPTGSTA
CEEEEEEECCCCCCCCCCEEEEEECCCHHHEEEEEEECCEEEEEEECCCEEEECCCCCCE
YSLSAGGPIVEPQVECSIITPLNPFKLEARPMVVSMDRRVEIDVDDPERAEVVVDGQEYM
EEECCCCCEECCCCEEEEECCCCCCEEECCCEEEEECCEEEEECCCCCCCEEEECCHHHC
NLDGTVSVTRSPNVARFIRFGSTYFERLKEKFLRWD
CCCCEEEEECCCCHHHHHHHCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014