Click here to switch to the map view.

The map label for this gene is IlvB_2 [H]

Identifier: 20093969

GI number: 20093969

Start: 495059

End: 496756

Strand: Direct

Name: IlvB_2 [H]

Synonym: MK0531

Alternate gene names: 20093969

Gene position: 495059-496756 (Clockwise)

Preceding gene: 20093968

Following gene: 20093970

Centisome position: 29.21

GC content: 58.6

Gene sequence:

>1698_bases
GTGAGTGAGTTGCCCACGGGTGCCAGGGTACTCGTCGAGTGCTTGAAAGAAGAGGGCGTCGAGCACATTTTCGGGTACCC
GGGAGGTGCCGTCCTACCGATTTATGACGAGATCTACGACGAGGTTTCGATCGAACACATCCTCGTACGACACGAGCAGG
GAGCTGCCCACGCCGCCGACGGGTACGCGAGGGTGAAAGGGAAACCCGGAGTCTGCATGGCGACATCGGGACCCGGAGCG
ACGAACCTAGTCACGGGTATCGCCACGGCTTACATGGACTCGTCCCCGGTAATAGCCATCACCGGACAGGTCCCCACCAC
CATGATCGGGAAGGACGCGTTCCAGGAGGTCGACGCCGTCGGCGTGTTCATGCCCATCACTAAGCATAACTACCAGATAG
GGAAACCCGAGGAGATACCGGAAGTCGTGAAGGAGGCGTTCAAGATAGCTATCACCGGTCGGCCGGGGCCTGTTCACATC
GACGTGCCCAAAGACGTGCAGGAAGCGGAAGTGGATGTCGAGATACCCAAAACCGTCGAGGTCGAGGGCTTGAACGTGGT
CAAGCGCGGTCATCCGGTACAGATCAAACGGGCCGCGGAGTTACTCGCCGAGGCGGAGCGACCCGTCATCTTAGCAGGCG
GTGGGTGTGTAATCTCCAACGCTACTCGGGAGCTGATCGAGCTCGCGGAACTGCTCGGTGCTCCGGTAGCTACGACCCTC
ATGGGGAAGGGAGCGTTTCCCGAGGATCATCCACTAGCACTGGGTATGGCCGGTATGCACGGCACTAAGGCCGCGAACTA
CGCGCTGACGGAGTGCGACGTGCTACTCGCTGTAGGGTGCAGGTTCTCGGACAGGACTACGGGGGATCCCTCCGGATTCG
CTCCCGAAGCGAAGATCATCCATATTGACATAGACCCCGCCGAGATCGGCAAGAACATCCCAGTCGACGTGCCCATAGTT
GGTGATGCGAAGCTCGTCCTCAGGGACCTGATTAAGGAGTTGAAGCGGCGTAAGTACCTGAGGGAACGTAAGCGCTGGGG
AGAGAGGATCGAGGAACTGAAGGCTGAAGTAGAGATGCCTCCGGAATCCACGGAGTCCGACCAACGGATCAGCCCGCGTG
AGCTCGTACGTGTGTTACACGAAGCCCTGAAAGATCGGGATTACATTCTCACTACGGACGTAGGTCAGAACCAGATGTGG
ATGGCCCGATACTTCCCGGTGGAAGAACCGCGCAGGTTCATCTCCTCCGGTGGTTTAGGAACCATGGGATTCGGTCTTCC
GGCCGCGCTCGGCGCTAAGGTAGCCGCACCGGAGAAAACCGTGGTCGCCGTGGTAGGAGACGGTGGGTTCTTGATGACCG
CGCAAGAGTTAGCCACCGCCGTGGACAACGACATCGAGGTGAAGGTCTTCGTCATGGATAATAGACTCTTAGGGATGGTA
GCGCAGTGGCAGAGGCTGTTCTACGACGAAAGATTGTCGGAATCGAAACTCGATGAGAAAACCGACATAGTGAAACTGAC
GGAGTCGTACGGAGCAGCCGGAATTACCGTGGAGGAGCCGAGCGAGCTGGAGTCCGCGGTAGAGGAAGCCTTCGAGACTC
CGGGTACGGTGGTGGTGGATGTGTTCGTAGATCCGGAAGAAATCATCCCGATGGTGCCGCCGGGAGGCGAGTTGCGCGAC
ATCCTTGGTGAAAAATGA

Upstream 100 bases:

>100_bases
GGTATGCGGATGTTCGGAGAGCCCGTCAAGTTGATCGACGGGACCAAGGTGTACGACCTCAAGGAATCTCGGCGGGAGGG
GAAGCCGGTCATACTGGAGG

Downstream 100 bases:

>100_bases
GGCGGAAGTGAAAAAAGGATGGAGGGGGCCTACCCCTCGGCCCCGACCTCTACCACACGCGGCACCAACATCGCTGTGCA
CATTTCAGGCATTTCGAGAG

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MSELPTGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAADGYARVKGKPGVCMATSGPGA
TNLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAVGVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHI
DVPKDVQEAEVDVEIPKTVEVEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGGCVISNATRELIELAELLGAPVATTL
MGKGAFPEDHPLALGMAGMHGTKAANYALTECDVLLAVGCRFSDRTTGDPSGFAPEAKIIHIDIDPAEIGKNIPVDVPIV
GDAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEVEMPPESTESDQRISPRELVRVLHEALKDRDYILTTDVGQNQMW
MARYFPVEEPRRFISSGGLGTMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMV
AQWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGTVVVDVFVDPEEIIPMVPPGGELRD
ILGEK

Sequences:

>Translated_565_residues
MSELPTGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAADGYARVKGKPGVCMATSGPGA
TNLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAVGVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHI
DVPKDVQEAEVDVEIPKTVEVEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGGCVISNATRELIELAELLGAPVATTL
MGKGAFPEDHPLALGMAGMHGTKAANYALTECDVLLAVGCRFSDRTTGDPSGFAPEAKIIHIDIDPAEIGKNIPVDVPIV
GDAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEVEMPPESTESDQRISPRELVRVLHEALKDRDYILTTDVGQNQMW
MARYFPVEEPRRFISSGGLGTMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMV
AQWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGTVVVDVFVDPEEIIPMVPPGGELRD
ILGEK
>Mature_564_residues
SELPTGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAADGYARVKGKPGVCMATSGPGAT
NLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAVGVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHID
VPKDVQEAEVDVEIPKTVEVEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGGCVISNATRELIELAELLGAPVATTLM
GKGAFPEDHPLALGMAGMHGTKAANYALTECDVLLAVGCRFSDRTTGDPSGFAPEAKIIHIDIDPAEIGKNIPVDVPIVG
DAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEVEMPPESTESDQRISPRELVRVLHEALKDRDYILTTDVGQNQMWM
ARYFPVEEPRRFISSGGLGTMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMVA
QWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGTVVVDVFVDPEEIIPMVPPGGELRDI
LGEK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=27.3049645390071, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI21361361, Length=504, Percent_Identity=26.5873015873016, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=46.3286713286713, Blast_Score=520, Evalue=1e-149,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=44.1281138790036, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1786717, Length=570, Percent_Identity=35.0877192982456, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI1787096, Length=557, Percent_Identity=31.2387791741472, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788716, Length=558, Percent_Identity=27.0609318996416, Blast_Score=195, Evalue=7e-51,
Organism=Caenorhabditis elegans, GI17531301, Length=559, Percent_Identity=27.906976744186, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=27.906976744186, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=569, Percent_Identity=27.2407732864675, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=40.8304498269896, Blast_Score=424, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=27.3049645390071, Blast_Score=163, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6321524, Length=514, Percent_Identity=26.8482490272374, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323073, Length=510, Percent_Identity=26.2745098039216, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=514, Percent_Identity=25.6809338521401, Blast_Score=110, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320123, Length=497, Percent_Identity=23.5412474849095, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19922626, Length=563, Percent_Identity=26.4653641207815, Blast_Score=181, Evalue=9e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61206; Mature: 61075

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELPTGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAAD
CCCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEHHHHHHHHHCHHHHEEECCCCCCCCCC
GYARVKGKPGVCMATSGPGATNLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAV
CEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCHHHHHHHHHC
GVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHIDVPKDVQEAEVDVEIPKTVE
EEEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHCEEEEECCCEEE
VEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGGCVISNATRELIELAELLGAPVATTL
ECCCCEEECCCCEEHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCCHHHHH
MGKGAFPEDHPLALGMAGMHGTKAANYALTECDVLLAVGCRFSDRTTGDPSGFAPEAKII
HCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEE
HIDIDPAEIGKNIPVDVPIVGDAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEVEMP
EEECCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PESTESDQRISPRELVRVLHEALKDRDYILTTDVGQNQMWMARYFPVEEPRRFISSGGLG
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCC
TMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMV
CCCCCCHHHHCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHCCCCEEEEEEECCHHHHHH
AQWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGTVVVD
HHHHHHHHHHHHHHHHCCCHHHHEEEHHHCCCCCEEECCHHHHHHHHHHHHCCCCCEEEE
VFVDPEEIIPMVPPGGELRDILGEK
EEECHHHCCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SELPTGARVLVECLKEEGVEHIFGYPGGAVLPIYDEIYDEVSIEHILVRHEQGAAHAAD
CCCCCHHHHHHHHHHHCCCCEECCCCCCCEEEHHHHHHHHHCHHHHEEECCCCCCCCCC
GYARVKGKPGVCMATSGPGATNLVTGIATAYMDSSPVIAITGQVPTTMIGKDAFQEVDAV
CEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHCHHHHHHHHHC
GVFMPITKHNYQIGKPEEIPEVVKEAFKIAITGRPGPVHIDVPKDVQEAEVDVEIPKTVE
EEEEEEECCCCCCCCCHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHCEEEEECCCEEE
VEGLNVVKRGHPVQIKRAAELLAEAERPVILAGGGCVISNATRELIELAELLGAPVATTL
ECCCCEEECCCCEEHHHHHHHHHHCCCCEEEECCCEEECHHHHHHHHHHHHHCCCHHHHH
MGKGAFPEDHPLALGMAGMHGTKAANYALTECDVLLAVGCRFSDRTTGDPSGFAPEAKII
HCCCCCCCCCCEEEEECCCCCCCCCCEEHHHHHEEEEECCCCCCCCCCCCCCCCCCCEEE
HIDIDPAEIGKNIPVDVPIVGDAKLVLRDLIKELKRRKYLRERKRWGERIEELKAEVEMP
EEECCHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PESTESDQRISPRELVRVLHEALKDRDYILTTDVGQNQMWMARYFPVEEPRRFISSGGLG
CCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCC
TMGFGLPAALGAKVAAPEKTVVAVVGDGGFLMTAQELATAVDNDIEVKVFVMDNRLLGMV
CCCCCCHHHHCCCCCCCCCEEEEEEECCCEEEEHHHHHHHHCCCCEEEEEEECCHHHHHH
AQWQRLFYDERLSESKLDEKTDIVKLTESYGAAGITVEEPSELESAVEEAFETPGTVVVD
HHHHHHHHHHHHHHHHCCCHHHHEEEHHHCCCCCEEECCHHHHHHHHHHHHCCCCCEEEE
VFVDPEEIIPMVPPGGELRDILGEK
EEECHHHCCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]