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The map label for this gene is vanS [H]

Identifier: 18311345

GI number: 18311345

Start: 2725258

End: 2727084

Strand: Reverse

Name: vanS [H]

Synonym: CPE2363

Alternate gene names: 18311345

Gene position: 2727084-2725258 (Counterclockwise)

Preceding gene: 18311346

Following gene: 18311344

Centisome position: 89.96

GC content: 25.4

Gene sequence:

>1827_bases
TTGGGTATAAAATTGACAAATAGTTTTTCAAGATGGTGGAAAAACTATAGCCTTTCAGTAAGAAGCATAGAAAATATGTT
TATAGTAATATTTGCTGTGTTTATACCTGTTTTATATATTATCTTATTTGGAGGATACTCCTTTGATAATATAGCAAAAT
TAAATTCTAAGGCAAATCCTGATATATGTAATAGAGTTATTTATAGGGATGCATTAATAAATCCACATAAGTATAAAGAG
TGTAGGTTATTAGCTCAATCTGTAGATATATCAGATTTTCTTTATAATGAGACTAACTATTTAAGCAAATTAGATAGCTT
AGAGACAAAGGAGCAAAAACAAGCATTTATTGCAAAGCTTCAAGAAAGGGCTACTAGTTATTCTGGTGTAGTTCTTATAA
ATAAGAAAACAGGAGAATATTACTCTAATAGAGTATGGTTTTATGAAAAACCTTATAATCTTTCAACGCCAAAAGAAGTT
TTAGAAAGTTTATCTAAAAATGATAATGTAGTCTTTAAAGAAATAAATTCTTCAGATAATTATGAAGAAATATATTTCTC
AAGAGGAGAACTTTATGATTCTGAAATAAATTCAATTAGAATTGCTTTTATAGCAACTATGACTACAACTCTTTTAATGT
TACTTTTAATCATAAAAAAATTAGTTATGATTAAAAATATGGGAATAAGAGCATATAAAGACAGCTGGAAAAATGGATAT
TTCTTTAGATTTTTAGATAGTTTAAATGTTTTAATAACAAAGAAGATGTTTATAGAAGAATTTTTAAAAGATAAGGTTGT
TATGTTTGTTATTTCATTTACTATAATATATATTCTTATAAATGGATTTATGAGAAATATAGAATTTGTATGGAAGCAAT
ATCACTATGTTTTAACAGACAATAGATACCTATGGATAATTCCAATTGTTTTAGTTATTCATTTTATAGTAAAGTTTATA
AGAAAATATGATGGATTAGATACTGTAATAAAAGGAATAGAAAAAGTTAAAAAAGGTGATACAGATATAGAGATAAAAGA
TAATGACGATAGACAAATAAGGGAATTAGCAGATGATGTTAATGATTTAAGACTAGGCTATAAAGAACTTGTTGAAGAAG
GTATAAAGAATGAAAAGTTAAAAACTGAGCTTATATCAAATGTTTCACATGATTTAAAAACTCCATTAACATCCATAATA
AATTATGTAAATATACTTCAGTTAAATAATATAAAGGAAGAGGAAAGAAAAGAATATATAGAAATATTAGATAAAAAATC
TCAAACCTTAAAGAAACTTATAGATGATTTGTTTGAAGTTTCTAAGATGTCTTCAGGAAAGGTAGAATTATTTAAATACA
ATATAGATATAATTCAACTTGTATATCAATGTATAGCTGAGGTAGAAGATGTATATTCAGAAAAGGAAATTGAATTTAAA
ATAAATGGGCCAGAGGAATGCATGGTTAAAATAGATCCTCAAAGAATGTCTAGGGTATTCCAAAACCTAACTACTAATGC
TTTAAAATATAGTTTAGAAAAAACTAGGGTCTATGTGGAAATAAAAGATTTAGAAAATTACGTGGAAATATCATTTAAAA
ATATTTCTTCTTATGCTTTAGATTTTGATGAGAAGGATATTTTAGAAAGATTTGCAAGAGGAGATGAATCAAGAAATTCA
ACCATAGAAGGATCTGGATTAGGTTTAGCAATAGCTAAAACTATTGTGGAACTTCATAATGGAAAGTTTAGAGTTGAATG
TGAAGGTGATTTGTTTAAAGCCTTTGTAATAATTCCTAAAAATAAAGATGATAATAATGAAGAATAA

Upstream 100 bases:

>100_bases
TATAAAAGTGAAAATACAGTAACAGTTCATATAAGAAGAATGAGAGAAAAAATAGAAATAAATTCGAAAGAACCTGAGTA
TATAAAGGTGGTGTGGGGAC

Downstream 100 bases:

>100_bases
TAAGATAGAGGTTGTTTAACTTAATTTTAATAAATTTAAAAAAATCTATAGCTGTAAAATTTTATGAAGGTTTTTAATTA
ACATATTTAAAAATGAAGTT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MGIKLTNSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFGGYSFDNIAKLNSKANPDICNRVIYRDALINPHKYKE
CRLLAQSVDISDFLYNETNYLSKLDSLETKEQKQAFIAKLQERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEV
LESLSKNDNVVFKEINSSDNYEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLIIKKLVMIKNMGIRAYKDSWKNGY
FFRFLDSLNVLITKKMFIEEFLKDKVVMFVISFTIIYILINGFMRNIEFVWKQYHYVLTDNRYLWIIPIVLVIHFIVKFI
RKYDGLDTVIKGIEKVKKGDTDIEIKDNDDRQIRELADDVNDLRLGYKELVEEGIKNEKLKTELISNVSHDLKTPLTSII
NYVNILQLNNIKEEERKEYIEILDKKSQTLKKLIDDLFEVSKMSSGKVELFKYNIDIIQLVYQCIAEVEDVYSEKEIEFK
INGPEECMVKIDPQRMSRVFQNLTTNALKYSLEKTRVYVEIKDLENYVEISFKNISSYALDFDEKDILERFARGDESRNS
TIEGSGLGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPKNKDDNNEE

Sequences:

>Translated_608_residues
MGIKLTNSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFGGYSFDNIAKLNSKANPDICNRVIYRDALINPHKYKE
CRLLAQSVDISDFLYNETNYLSKLDSLETKEQKQAFIAKLQERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEV
LESLSKNDNVVFKEINSSDNYEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLIIKKLVMIKNMGIRAYKDSWKNGY
FFRFLDSLNVLITKKMFIEEFLKDKVVMFVISFTIIYILINGFMRNIEFVWKQYHYVLTDNRYLWIIPIVLVIHFIVKFI
RKYDGLDTVIKGIEKVKKGDTDIEIKDNDDRQIRELADDVNDLRLGYKELVEEGIKNEKLKTELISNVSHDLKTPLTSII
NYVNILQLNNIKEEERKEYIEILDKKSQTLKKLIDDLFEVSKMSSGKVELFKYNIDIIQLVYQCIAEVEDVYSEKEIEFK
INGPEECMVKIDPQRMSRVFQNLTTNALKYSLEKTRVYVEIKDLENYVEISFKNISSYALDFDEKDILERFARGDESRNS
TIEGSGLGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPKNKDDNNEE
>Mature_607_residues
GIKLTNSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFGGYSFDNIAKLNSKANPDICNRVIYRDALINPHKYKEC
RLLAQSVDISDFLYNETNYLSKLDSLETKEQKQAFIAKLQERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEVL
ESLSKNDNVVFKEINSSDNYEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLIIKKLVMIKNMGIRAYKDSWKNGYF
FRFLDSLNVLITKKMFIEEFLKDKVVMFVISFTIIYILINGFMRNIEFVWKQYHYVLTDNRYLWIIPIVLVIHFIVKFIR
KYDGLDTVIKGIEKVKKGDTDIEIKDNDDRQIRELADDVNDLRLGYKELVEEGIKNEKLKTELISNVSHDLKTPLTSIIN
YVNILQLNNIKEEERKEYIEILDKKSQTLKKLIDDLFEVSKMSSGKVELFKYNIDIIQLVYQCIAEVEDVYSEKEIEFKI
NGPEECMVKIDPQRMSRVFQNLTTNALKYSLEKTRVYVEIKDLENYVEISFKNISSYALDFDEKDILERFARGDESRNST
IEGSGLGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPKNKDDNNEE

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=257, Percent_Identity=27.6264591439689, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=28.2051282051282, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1786600, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786783, Length=294, Percent_Identity=20.7482993197279, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI1786912, Length=239, Percent_Identity=25.9414225941423, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=285, Percent_Identity=25.9649122807018, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1788279, Length=261, Percent_Identity=26.8199233716475, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI87082128, Length=210, Percent_Identity=27.1428571428571, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1789149, Length=209, Percent_Identity=22.488038277512, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1790346, Length=257, Percent_Identity=24.124513618677, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=20.5761316872428, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=25.2, Blast_Score=64, Evalue=4e-11,
Organism=Escherichia coli, GI1788549, Length=245, Percent_Identity=24.0816326530612, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71255; Mature: 71124

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKLTNSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFGGYSFDNIAKLNSKANP
CCCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCH
DICNRVIYRDALINPHKYKECRLLAQSVDISDFLYNETNYLSKLDSLETKEQKQAFIAKL
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEVLESLSKNDNVVFKEINSSDN
HHHCCCCCCEEEEECCCCCHHHCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCC
YEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLIIKKLVMIKNMGIRAYKDSWKNGY
HHHHEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCH
FFRFLDSLNVLITKKMFIEEFLKDKVVMFVISFTIIYILINGFMRNIEFVWKQYHYVLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
NRYLWIIPIVLVIHFIVKFIRKYDGLDTVIKGIEKVKKGDTDIEIKDNDDRQIRELADDV
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
NDLRLGYKELVEEGIKNEKLKTELISNVSHDLKTPLTSIINYVNILQLNNIKEEERKEYI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILDKKSQTLKKLIDDLFEVSKMSSGKVELFKYNIDIIQLVYQCIAEVEDVYSEKEIEFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEE
INGPEECMVKIDPQRMSRVFQNLTTNALKYSLEKTRVYVEIKDLENYVEISFKNISSYAL
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHCCHHHHC
DFDEKDILERFARGDESRNSTIEGSGLGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPK
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEC
NKDDNNEE
CCCCCCCC
>Mature Secondary Structure 
GIKLTNSFSRWWKNYSLSVRSIENMFIVIFAVFIPVLYIILFGGYSFDNIAKLNSKANP
CCEECCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCH
DICNRVIYRDALINPHKYKECRLLAQSVDISDFLYNETNYLSKLDSLETKEQKQAFIAKL
HHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
QERATSYSGVVLINKKTGEYYSNRVWFYEKPYNLSTPKEVLESLSKNDNVVFKEINSSDN
HHHCCCCCCEEEEECCCCCHHHCEEEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCC
YEEIYFSRGELYDSEINSIRIAFIATMTTTLLMLLLIIKKLVMIKNMGIRAYKDSWKNGY
HHHHEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCH
FFRFLDSLNVLITKKMFIEEFLKDKVVMFVISFTIIYILINGFMRNIEFVWKQYHYVLTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
NRYLWIIPIVLVIHFIVKFIRKYDGLDTVIKGIEKVKKGDTDIEIKDNDDRQIRELADDV
CCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
NDLRLGYKELVEEGIKNEKLKTELISNVSHDLKTPLTSIINYVNILQLNNIKEEERKEYI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
EILDKKSQTLKKLIDDLFEVSKMSSGKVELFKYNIDIIQLVYQCIAEVEDVYSEKEIEFK
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCEEEEE
INGPEECMVKIDPQRMSRVFQNLTTNALKYSLEKTRVYVEIKDLENYVEISFKNISSYAL
ECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHHHCCHHHHC
DFDEKDILERFARGDESRNSTIEGSGLGLAIAKTIVELHNGKFRVECEGDLFKAFVIIPK
CCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEEC
NKDDNNEE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]