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The map label for this gene is nrdF [H]

Identifier: 18311342

GI number: 18311342

Start: 2721085

End: 2722119

Strand: Reverse

Name: nrdF [H]

Synonym: CPE2360

Alternate gene names: 18311342

Gene position: 2722119-2721085 (Counterclockwise)

Preceding gene: 18311343

Following gene: 18311341

Centisome position: 89.8

GC content: 28.79

Gene sequence:

>1035_bases
ATGGAAATAAATAAAAGACCGCTTTTTAATGAGTTTGGAGATATTGAAACTAATAAAAAGAAAATGATAAACGGTAATAC
AACTAATTTAAATGACTTTAATAATATGAAATATACTTGGGTTTCAGAATGGTACAGACAAGCAATGAATAATTTCTGGA
TACCAGAGGAAATAAACTTAGCTCAAGACTTAAAAGATTATAAAAAGTTGACTAAGGAAGAAAAAACAGCATATGATAAA
ATCTTATCTTTCTTAATATTTTTAGATTCAATACAAACAGCTAACTTAGGAAATATAAATAGTTATATTACTGCTTCAGA
AGTTAACTTATGTTTAACCATACAAGCTTTCCAAGAAGCGGTTCACTCACAAAGTTATAGTTATATGCTAGATAGTATTT
GTTCACCAGAGGAGAGAAACGAAATATTATTCCAATGGAAAGACGATGCTATATTACTTCAAAGAAATAAATTTATAGGA
GACTTATATAATAACTTCTTAGAAGATTCAAGCATGGAAAACTTTATAAAATCAGTAATGGCTAACTATATTTTAGAGGG
TGTTTATTTCTATTCAGGATTTATGTTTTTCTATAACTTAGAGAGAAATGGAAAAATGCCTGGTTCAGCTCAAGAGATTA
GATATATAAACAGAGATGAAAATACTCATTTATGGTTATTTAGAAGTATAATAAAAGAATTAAAAGAAGAAATACCAGAA
GTATTCACTAAAGAGTTAAAAGAAGAATTAAGAGAAATGGTTAGAACTGGTGTTGAACATGAAATTGCATGGGGGCACTA
TGTTATAGGTGATAATGTTACTGGAATAAATAAAAACCTTATAGAGAGATATATAAAATACATAGGAAACTTAAGAGTTA
AAGCTATAGGTTTAGAGCCTTTATTTGAAGGCTACAATGAGAATCCAGCACCATGGGTAGATTATTATGCTGATGCAAAT
CAAGTAAAAACAGACTTCTTTGAGGCAAAATCAACAGCATATGCTAAAGCAGGAGCCTTAATAGACGATCTATAA

Upstream 100 bases:

>100_bases
CTTAGAATATAAATTAAGAATTTTAAAGATACTCATCTAGGCTTAGGTGAGTATCTATTAATTATAGATAGAGAAATAAA
GAGATGAAAGGTGACGTAAA

Downstream 100 bases:

>100_bases
AAATAGAATTTAATATTTAACTGCATAAATTATAAAAGAGAGCTATATCAATACTATTTATGAGAGAATTTATAAATATA
AAATTTATGAATTCTTATAA

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEWYRQAMNNFWIPEEINLAQDLKDYKKLTKEEKTAYDK
ILSFLIFLDSIQTANLGNINSYITASEVNLCLTIQAFQEAVHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIG
DLYNNFLEDSSMENFIKSVMANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINRDENTHLWLFRSIIKELKEEIPE
VFTKELKEELREMVRTGVEHEIAWGHYVIGDNVTGINKNLIERYIKYIGNLRVKAIGLEPLFEGYNENPAPWVDYYADAN
QVKTDFFEAKSTAYAKAGALIDDL

Sequences:

>Translated_344_residues
MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEWYRQAMNNFWIPEEINLAQDLKDYKKLTKEEKTAYDK
ILSFLIFLDSIQTANLGNINSYITASEVNLCLTIQAFQEAVHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIG
DLYNNFLEDSSMENFIKSVMANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINRDENTHLWLFRSIIKELKEEIPE
VFTKELKEELREMVRTGVEHEIAWGHYVIGDNVTGINKNLIERYIKYIGNLRVKAIGLEPLFEGYNENPAPWVDYYADAN
QVKTDFFEAKSTAYAKAGALIDDL
>Mature_344_residues
MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEWYRQAMNNFWIPEEINLAQDLKDYKKLTKEEKTAYDK
ILSFLIFLDSIQTANLGNINSYITASEVNLCLTIQAFQEAVHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIG
DLYNNFLEDSSMENFIKSVMANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINRDENTHLWLFRSIIKELKEEIPE
VFTKELKEELREMVRTGVEHEIAWGHYVIGDNVTGINKNLIERYIKYIGNLRVKAIGLEPLFEGYNENPAPWVDYYADAN
QVKTDFFEAKSTAYAKAGALIDDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=336, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI289177074, Length=336, Percent_Identity=24.1071428571429, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4557845, Length=313, Percent_Identity=25.2396166134185, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI260064013, Length=313, Percent_Identity=25.2396166134185, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI289177076, Length=291, Percent_Identity=24.3986254295533, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788567, Length=351, Percent_Identity=25.0712250712251, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789031, Length=303, Percent_Identity=25.4125412541254, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17551968, Length=338, Percent_Identity=22.189349112426, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI193206379, Length=259, Percent_Identity=23.1660231660232, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322434, Length=311, Percent_Identity=25.08038585209, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321619, Length=306, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18079279, Length=314, Percent_Identity=24.203821656051, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40369; Mature: 40369

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEWYRQAMNNFWIPEEINL
CCCCCCCCCHHCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
AQDLKDYKKLTKEEKTAYDKILSFLIFLDSIQTANLGNINSYITASEVNLCLTIQAFQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHH
VHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIGDLYNNFLEDSSMENFIKSVM
HHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
ANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINRDENTHLWLFRSIIKELKEEIPE
HHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
VFTKELKEELREMVRTGVEHEIAWGHYVIGDNVTGINKNLIERYIKYIGNLRVKAIGLEP
HHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCH
LFEGYNENPAPWVDYYADANQVKTDFFEAKSTAYAKAGALIDDL
HHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure
MEINKRPLFNEFGDIETNKKKMINGNTTNLNDFNNMKYTWVSEWYRQAMNNFWIPEEINL
CCCCCCCCCHHCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCH
AQDLKDYKKLTKEEKTAYDKILSFLIFLDSIQTANLGNINSYITASEVNLCLTIQAFQEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHH
VHSQSYSYMLDSICSPEERNEILFQWKDDAILLQRNKFIGDLYNNFLEDSSMENFIKSVM
HHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHH
ANYILEGVYFYSGFMFFYNLERNGKMPGSAQEIRYINRDENTHLWLFRSIIKELKEEIPE
HHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
VFTKELKEELREMVRTGVEHEIAWGHYVIGDNVTGINKNLIERYIKYIGNLRVKAIGLEP
HHHHHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEECCCH
LFEGYNENPAPWVDYYADANQVKTDFFEAKSTAYAKAGALIDDL
HHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]