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The map label for this gene is epsF [H]

Identifier: 18311267

GI number: 18311267

Start: 2628890

End: 2630095

Strand: Reverse

Name: epsF [H]

Synonym: CPE2285

Alternate gene names: 18311267

Gene position: 2630095-2628890 (Counterclockwise)

Preceding gene: 18311268

Following gene: 18311266

Centisome position: 86.76

GC content: 30.18

Gene sequence:

>1206_bases
ATGGCAAATTTTAAATATAAAGCTATTAATTCAGAAGGGCAAAGAATAGAAGGTTCTCAAAGTGCTGATTCTGAAAGCCA
AGTTAGGGAAATGCTTTTATCAAATCAATATTATCCTCTAAGTATAGAAAAAGAAAATAGTAAAAATAAAATCAGTTTTT
CTTTTAATCGTAAGGTAAAGCTTAAGGACATAGCAATATTTTGTAGACAGTTTTATGTTATGTTAGATTCTGGATTATCT
ATTGGTAAGGCTTTAAATATATTAATAGAGCAAGGTGAGAAGCCAAAGATTAGGGAAGCCTTAATTGGAGTAAATGGAGA
TTTAAAAAGAGGGGAAACTCTAGCAAACTCCATGAGAAAAAGAAAGGATGTTTTTCCAAACTTACTTACTAGCATGATTG
ATGCTGGTGAGAGAAGTGGTAATTTAGATATAATCTTAAAAAGAATGGCTGAGTACTATGAAAAAGAGACAAAGATAAGA
GGAAAGATTAAAAGTGCTATGATTTATCCTATAGTTCTTGGAGTGGTAGCTATTATTGCTATTACTTTTATTCTTACCTT
TGTAATGCCAACCTTTGTGCAGATGTTTGAAGAAAATAATGTAGATCTTCCAATGTCAACCAAGATGGTTCTTGGGACAA
GCAAAATGTTAGGTAAGTATGGAATAATAATTTTTTTAATTTTAGTAACAGCAATTATATTACTTGGCAAATATTTAAAG
AGTGAAGAGGGTCAATATAAGCTAAGTATAATAAACTTAAAAATTCCAGTGATAAAGAAATTAACTCAAAAAATAATAGT
TTCTAGATTTACAAGAACAATGGGAATAGTATCATCAAGTGGAATGTCTTTAGTAACCTCTCTAGAAATAGTTGCCTCTG
TAGTTGGAAATAAAATTGCAGAGAAGGAATTACTAAAAGTAAAAGAAAAGGTCTTAAAAGGTGAGGGGTTAGGAGATTCA
ATAATGAAAATAAAAATTTTCCCACCAATGCTTGCTTCCATGGTTAAGGTTGGAGAAGAAGCAGGTTCACTAGATAGCAT
ATTAGATAAAACAGCAGATTTTTATGATGATGAACTAGAAAGAGAAATACAAACAGCAACAGCCTTAATAGAGCCAGCAA
TGATAGTTGTTATGGGAATAATTATAGGATTTTTACTAATTTCAATATTAACACCTATGTTTAAAATGTATAACAGTATA
GGTTAA

Upstream 100 bases:

>100_bases
AATCATGTAGAAGTTTAGTCTTGGAGGGGAATACCACAGTTGAAGAAATGTTAAATATAACTTATGATATGTAATTTGGT
GAAAGAAAAGGAGAGGAATT

Downstream 100 bases:

>100_bases
AAGCATAATTAATAAGAATTAAAAGTTTAGCTTTTAATATAAATTACATATTATTTTAAGGAGGAAAACCAATGAATACA
AAAAAACAAAACAAAAAAAA

Product: type II secretion system protein F

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEKENSKNKISFSFNRKVKLKDIAIFCRQFYVMLDSGLS
IGKALNILIEQGEKPKIREALIGVNGDLKRGETLANSMRKRKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIR
GKIKSAMIYPIVLGVVAIIAITFILTFVMPTFVQMFEENNVDLPMSTKMVLGTSKMLGKYGIIIFLILVTAIILLGKYLK
SEEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGIVSSSGMSLVTSLEIVASVVGNKIAEKELLKVKEKVLKGEGLGDS
IMKIKIFPPMLASMVKVGEEAGSLDSILDKTADFYDDELEREIQTATALIEPAMIVVMGIIIGFLLISILTPMFKMYNSI
G

Sequences:

>Translated_401_residues
MANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEKENSKNKISFSFNRKVKLKDIAIFCRQFYVMLDSGLS
IGKALNILIEQGEKPKIREALIGVNGDLKRGETLANSMRKRKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIR
GKIKSAMIYPIVLGVVAIIAITFILTFVMPTFVQMFEENNVDLPMSTKMVLGTSKMLGKYGIIIFLILVTAIILLGKYLK
SEEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGIVSSSGMSLVTSLEIVASVVGNKIAEKELLKVKEKVLKGEGLGDS
IMKIKIFPPMLASMVKVGEEAGSLDSILDKTADFYDDELEREIQTATALIEPAMIVVMGIIIGFLLISILTPMFKMYNSI
G
>Mature_400_residues
ANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEKENSKNKISFSFNRKVKLKDIAIFCRQFYVMLDSGLSI
GKALNILIEQGEKPKIREALIGVNGDLKRGETLANSMRKRKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIRG
KIKSAMIYPIVLGVVAIIAITFILTFVMPTFVQMFEENNVDLPMSTKMVLGTSKMLGKYGIIIFLILVTAIILLGKYLKS
EEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGIVSSSGMSLVTSLEIVASVVGNKIAEKELLKVKEKVLKGEGLGDSI
MKIKIFPPMLASMVKVGEEAGSLDSILDKTADFYDDELEREIQTATALIEPAMIVVMGIIIGFLLISILTPMFKMYNSIG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=405, Percent_Identity=26.9135802469136, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1786295, Length=403, Percent_Identity=23.0769230769231, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44705; Mature: 44574

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEKENSKNKISFSFNRKVK
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCEEE
LKDIAIFCRQFYVMLDSGLSIGKALNILIEQGEKPKIREALIGVNGDLKRGETLANSMRK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
RKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIRGKIKSAMIYPIVLGVVAIIA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITFILTFVMPTFVQMFEENNVDLPMSTKMVLGTSKMLGKYGIIIFLILVTAIILLGKYLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGIVSSSGMSLVTSLEIVASVVGNKIA
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHH
EKELLKVKEKVLKGEGLGDSIMKIKIFPPMLASMVKVGEEAGSLDSILDKTADFYDDELE
HHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
REIQTATALIEPAMIVVMGIIIGFLLISILTPMFKMYNSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANFKYKAINSEGQRIEGSQSADSESQVREMLLSNQYYPLSIEKENSKNKISFSFNRKVK
CCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCEEE
LKDIAIFCRQFYVMLDSGLSIGKALNILIEQGEKPKIREALIGVNGDLKRGETLANSMRK
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHH
RKDVFPNLLTSMIDAGERSGNLDIILKRMAEYYEKETKIRGKIKSAMIYPIVLGVVAIIA
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITFILTFVMPTFVQMFEENNVDLPMSTKMVLGTSKMLGKYGIIIFLILVTAIILLGKYLK
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEEGQYKLSIINLKIPVIKKLTQKIIVSRFTRTMGIVSSSGMSLVTSLEIVASVVGNKIA
CCCCCEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHH
EKELLKVKEKVLKGEGLGDSIMKIKIFPPMLASMVKVGEEAGSLDSILDKTADFYDDELE
HHHHHHHHHHHHCCCCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
REIQTATALIEPAMIVVMGIIIGFLLISILTPMFKMYNSIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]