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The map label for this gene is mnmC

Identifier: 17546428

GI number: 17546428

Start: 1824638

End: 1826611

Strand: Direct

Name: mnmC

Synonym: RSc1709

Alternate gene names: 17546428

Gene position: 1824638-1826611 (Clockwise)

Preceding gene: 17546427

Following gene: 17546429

Centisome position: 49.1

GC content: 73.76

Gene sequence:

>1974_bases
ATGCCTCGCGGCCTCATTCTCGCCACACCTCAACTGCCCCCGGACGGCACGCCGTTCTCCGCCCGGTACGACGACGTCTA
CCACAGCACCGAAGGCGGCCTGGCGCAGGCGCATCACGTGTTCCTGGGCGGCAACGGCCTGCCCGGCGGGTGGCAGGGCA
AGCGCAGCTTCACCATCGTGGAGACCGGCTTCGGGCAGGGCCTGAACTTCCTGGCCACCTGGGCGGCCTGGCGCGACGAT
CCGCAGCGCTGCGGGCGTCTCGATTTCGTTTCGGTCGAAAAGCACCCGTTCGATCGCGCCGGGCTGGCCCTCGTGCACGC
GCAGGAGGGCGCGCTGGCCTCGCTCGCCGAGGCGTTGCGTCAGGCGTGGCCGGTGCCGGTGCCGGGCGTGCACCGCCTCG
TCTTCGATGCGGGGCGTGTGACGCTCACCCTGATGCTGGGGGACGCGGAAACGCTGCTGCCGCAGCTGTCCTGCGCCGCC
GATGCCTTCTACCTGGACGGCTTCTCACCCGCCAAGAACCCTGATCTGTGGCAGCCGGGCGTGTTCAAGCAGCTCGCCCG
CATCGCGCGCGGCGGTGCGACGCTGGCAACCTATACGGCGGCCGGCGCCGTGCGCCACGGCTTGCGCGAGGCGGGTTTCG
AGGTGCGCAAGGCGCCCGGCTTCGGCCGCAAGCGCGACATGACCGTCGCCGCATTCCCGACGCACTGGCGGACCCGGCGC
GGGCCGGCGGAGGCACCGGTCTGGCCGGAGCGCCACGCCATCGTGCTGGGCGCCGGGCTGGCGGGCTGCTCGGTGGCGGA
GCGGCTGGCCGCGCGCGGCTGGCGCATCACCCTGATCGACGAGCACGAGGCGCCGGCAGGCGGCGCCTCGGCGCACCGGG
CGGCGGCCATGCATCCGCACGTGTCGATCGATGACAGCGTGCTCTCGCGCCTGTCCCGCGCGGGCAACCTGCTGGCGCGT
CGCCATTGGGAGGCGCTCGACCGCGCCGGCTTTGCCACCGGTTTCCACCCGACCGGCGTGCTGCAGTTGGCCGAGCACGC
CGACGATGCCGCCGAGCAGCAGCGCATCGTCCAGGCGCTCGGTTTCCCGGCCGATTTCATCGCGTGGCTGGACGCCGCCC
GCGCGGCCGATCGTGTCGGCGCGGGCGTGCCGCAGGGGGGCTGGTGGTTCGCGCAGGCCGGCTGGGTGGCGCCGCCCGAC
ATCTGCCGCGCAGCCCTCGCCGCGGCAGGTGAGGCCGTCGAGCTGCGGTGGCGCACCCGGATCGAATCCCTGCTGCGGCA
TGACGCCGGGTGGCGGGCCTGCGACGCGCAGGGCAACGTGATTGCGCACGCACCGGTCGTCGTGCTCGCCAACAGTCTGG
ATGCGGTCCGGCTGGCGCCGCTGGCATCCGCCGCGCTCAAGCCGGTGCGCGGCCAGCTGACGGATGTGCCGGTTGCCGCG
CTCGAAGACGGCGTGCCATGGCCTCGCGCCGTCGTCTGCGGCGACGGCTATCTGTTGCCCCGGGAGCGCGGAGCCCCGAC
GGTGCGCGTGGGTTCCTCTTTCCAGCCGGGCGAGACCGATCCCGACGCGCGTACCGCCGACCACGCCGCCAACCTGCGCC
GGCTGGCCGGGCTGCAGCCCGCGCATGCCGCCGCCCTGGCCCGCGTGGACCCGGCGCGGCTCCTTGGCTACGTGGGCGTG
CGCTGCGTCTCCGCCAACCGGTTGCCGCTGATTGGCGCCGTGGCGGACGAGGCAGCGATCATGGCGCCCGGCTTCCGCTT
GCGCGGACCGCATGCCGCGCTGCCGCGCGTGCCTGGGCTCTATGCCGCGCTGGCCTATGGCTCACGCGGCCTCACGTGGT
CGGTGCTGGGCGCGGAGCTGCTCGCGGCGCAGATCGACGGCGGCCCGCTTCCGCTCGAATCCGAACTGGCAGCCGCATTG
GACCCCGGTCGTTTCCTGATGCGGGCGTTGCGCCACGGCAAGCACGCGGCATGA

Upstream 100 bases:

>100_bases
GTCGCCACACCCGTGGCGATGCCGACTAGCCGTTGCGGCGGATCAAGCCGAGGCCTCGGGGGAGGGCGATACAATCCGGC
GCTTCCCGGACGGATTGCCC

Downstream 100 bases:

>100_bases
TTGCCCACAGCCATCGATTGACGAAGTGCGGCCGAAGCGGTTTGCCGGGCTGCCACCCCCAAAAAGTCAGGGCTGGCGAG
CGTTCCATGCCATAATATGC

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 657; Mature: 656

Protein sequence:

>657_residues
MPRGLILATPQLPPDGTPFSARYDDVYHSTEGGLAQAHHVFLGGNGLPGGWQGKRSFTIVETGFGQGLNFLATWAAWRDD
PQRCGRLDFVSVEKHPFDRAGLALVHAQEGALASLAEALRQAWPVPVPGVHRLVFDAGRVTLTLMLGDAETLLPQLSCAA
DAFYLDGFSPAKNPDLWQPGVFKQLARIARGGATLATYTAAGAVRHGLREAGFEVRKAPGFGRKRDMTVAAFPTHWRTRR
GPAEAPVWPERHAIVLGAGLAGCSVAERLAARGWRITLIDEHEAPAGGASAHRAAAMHPHVSIDDSVLSRLSRAGNLLAR
RHWEALDRAGFATGFHPTGVLQLAEHADDAAEQQRIVQALGFPADFIAWLDAARAADRVGAGVPQGGWWFAQAGWVAPPD
ICRAALAAAGEAVELRWRTRIESLLRHDAGWRACDAQGNVIAHAPVVVLANSLDAVRLAPLASAALKPVRGQLTDVPVAA
LEDGVPWPRAVVCGDGYLLPRERGAPTVRVGSSFQPGETDPDARTADHAANLRRLAGLQPAHAAALARVDPARLLGYVGV
RCVSANRLPLIGAVADEAAIMAPGFRLRGPHAALPRVPGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESELAAAL
DPGRFLMRALRHGKHAA

Sequences:

>Translated_657_residues
MPRGLILATPQLPPDGTPFSARYDDVYHSTEGGLAQAHHVFLGGNGLPGGWQGKRSFTIVETGFGQGLNFLATWAAWRDD
PQRCGRLDFVSVEKHPFDRAGLALVHAQEGALASLAEALRQAWPVPVPGVHRLVFDAGRVTLTLMLGDAETLLPQLSCAA
DAFYLDGFSPAKNPDLWQPGVFKQLARIARGGATLATYTAAGAVRHGLREAGFEVRKAPGFGRKRDMTVAAFPTHWRTRR
GPAEAPVWPERHAIVLGAGLAGCSVAERLAARGWRITLIDEHEAPAGGASAHRAAAMHPHVSIDDSVLSRLSRAGNLLAR
RHWEALDRAGFATGFHPTGVLQLAEHADDAAEQQRIVQALGFPADFIAWLDAARAADRVGAGVPQGGWWFAQAGWVAPPD
ICRAALAAAGEAVELRWRTRIESLLRHDAGWRACDAQGNVIAHAPVVVLANSLDAVRLAPLASAALKPVRGQLTDVPVAA
LEDGVPWPRAVVCGDGYLLPRERGAPTVRVGSSFQPGETDPDARTADHAANLRRLAGLQPAHAAALARVDPARLLGYVGV
RCVSANRLPLIGAVADEAAIMAPGFRLRGPHAALPRVPGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESELAAAL
DPGRFLMRALRHGKHAA
>Mature_656_residues
PRGLILATPQLPPDGTPFSARYDDVYHSTEGGLAQAHHVFLGGNGLPGGWQGKRSFTIVETGFGQGLNFLATWAAWRDDP
QRCGRLDFVSVEKHPFDRAGLALVHAQEGALASLAEALRQAWPVPVPGVHRLVFDAGRVTLTLMLGDAETLLPQLSCAAD
AFYLDGFSPAKNPDLWQPGVFKQLARIARGGATLATYTAAGAVRHGLREAGFEVRKAPGFGRKRDMTVAAFPTHWRTRRG
PAEAPVWPERHAIVLGAGLAGCSVAERLAARGWRITLIDEHEAPAGGASAHRAAAMHPHVSIDDSVLSRLSRAGNLLARR
HWEALDRAGFATGFHPTGVLQLAEHADDAAEQQRIVQALGFPADFIAWLDAARAADRVGAGVPQGGWWFAQAGWVAPPDI
CRAALAAAGEAVELRWRTRIESLLRHDAGWRACDAQGNVIAHAPVVVLANSLDAVRLAPLASAALKPVRGQLTDVPVAAL
EDGVPWPRAVVCGDGYLLPRERGAPTVRVGSSFQPGETDPDARTADHAANLRRLAGLQPAHAAALARVDPARLLGYVGVR
CVSANRLPLIGAVADEAAIMAPGFRLRGPHAALPRVPGLYAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESELAAALD
PGRFLMRALRHGKHAA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=669, Percent_Identity=35.8744394618834, Blast_Score=357, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_RALSO (Q8XYP9)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519830.1
- ProteinModelPortal:   Q8XYP9
- SMR:   Q8XYP9
- GeneID:   1220545
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc1709
- NMPDR:   fig|267608.1.peg.1709
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- ProtClustDB:   PRK01747
- BioCyc:   RSOL267608:RSC1709-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 69668; Mature: 69537

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRGLILATPQLPPDGTPFSARYDDVYHSTEGGLAQAHHVFLGGNGLPGGWQGKRSFTIV
CCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
ETGFGQGLNFLATWAAWRDDPQRCGRLDFVSVEKHPFDRAGLALVHAQEGALASLAEALR
ECCCCCCHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHH
QAWPVPVPGVHRLVFDAGRVTLTLMLGDAETLLPQLSCAADAFYLDGFSPAKNPDLWQPG
HHCCCCCCCHHHHHEECCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCH
VFKQLARIARGGATLATYTAAGAVRHGLREAGFEVRKAPGFGRKRDMTVAAFPTHWRTRR
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCHHCCC
GPAEAPVWPERHAIVLGAGLAGCSVAERLAARGWRITLIDEHEAPAGGASAHRAAAMHPH
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VSIDDSVLSRLSRAGNLLARRHWEALDRAGFATGFHPTGVLQLAEHADDAAEQQRIVQAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
GFPADFIAWLDAARAADRVGAGVPQGGWWFAQAGWVAPPDICRAALAAAGEAVELRWRTR
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEHHHHHH
IESLLRHDAGWRACDAQGNVIAHAPVVVLANSLDAVRLAPLASAALKPVRGQLTDVPVAA
HHHHHHHCCCCEEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LEDGVPWPRAVVCGDGYLLPRERGAPTVRVGSSFQPGETDPDARTADHAANLRRLAGLQP
HHCCCCCCCEEEECCCEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCH
AHAAALARVDPARLLGYVGVRCVSANRLPLIGAVADEAAIMAPGFRLRGPHAALPRVPGL
HHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCCEEECCCCEECCCCCCCCCCCHH
YAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESELAAALDPGRFLMRALRHGKHAA
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PRGLILATPQLPPDGTPFSARYDDVYHSTEGGLAQAHHVFLGGNGLPGGWQGKRSFTIV
CCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEE
ETGFGQGLNFLATWAAWRDDPQRCGRLDFVSVEKHPFDRAGLALVHAQEGALASLAEALR
ECCCCCCHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHH
QAWPVPVPGVHRLVFDAGRVTLTLMLGDAETLLPQLSCAADAFYLDGFSPAKNPDLWQPG
HHCCCCCCCHHHHHEECCCEEEEEEECCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCH
VFKQLARIARGGATLATYTAAGAVRHGLREAGFEVRKAPGFGRKRDMTVAAFPTHWRTRR
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEECCCCHHCCC
GPAEAPVWPERHAIVLGAGLAGCSVAERLAARGWRITLIDEHEAPAGGASAHRAAAMHPH
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHCCCC
VSIDDSVLSRLSRAGNLLARRHWEALDRAGFATGFHPTGVLQLAEHADDAAEQQRIVQAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHH
GFPADFIAWLDAARAADRVGAGVPQGGWWFAQAGWVAPPDICRAALAAAGEAVELRWRTR
CCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCCEEHHHHHH
IESLLRHDAGWRACDAQGNVIAHAPVVVLANSLDAVRLAPLASAALKPVRGQLTDVPVAA
HHHHHHHCCCCEEECCCCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
LEDGVPWPRAVVCGDGYLLPRERGAPTVRVGSSFQPGETDPDARTADHAANLRRLAGLQP
HHCCCCCCCEEEECCCEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCH
AHAAALARVDPARLLGYVGVRCVSANRLPLIGAVADEAAIMAPGFRLRGPHAALPRVPGL
HHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCCEEECCCCEECCCCCCCCCCCHH
YAALAYGSRGLTWSVLGAELLAAQIDGGPLPLESELAAALDPGRFLMRALRHGKHAA
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852