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The map label for this gene is nifS [H]

Identifier: 17545738

GI number: 17545738

Start: 1072986

End: 1074203

Strand: Direct

Name: nifS [H]

Synonym: RSc1019

Alternate gene names: 17545738

Gene position: 1072986-1074203 (Clockwise)

Preceding gene: 17545737

Following gene: 17545739

Centisome position: 28.87

GC content: 64.61

Gene sequence:

>1218_bases
ATGAGTACCCCACATCTCCCCATCTACATGGACTATTCCGCGACGACGCCGGTGGATCCGCGCGTGGTCGACAAGATGAT
TCCGTACCTGCGCGAGCAGTTCGGCAATCCGGCGTCGCGCAGCCACGCGTTCGGCTGGGAAGCGGAAAAGGCCGTCGAAA
CCGCGCGGGAACAGGTGGCGGCGCTGGTCAATGCCGACCCGCGCGAGATCGTGTGGACGTCCGGCGCGACCGAGTCCGAC
AACCTGGCGCTCAAGGGTGCGGCCAACTTCTACGGCAGCAAGGGCAAGCACATCATCACCGTCAAGACCGAGCACAAGGC
CGTGCTCGATACGGCGCGCGAGTTGGAGCGCCAGGGCTTCGAGGTGACCTACCTTGACGTGCAGGAAAACGGCCTGATCG
ACATCGAGCAGTTCAAGCAGGCGCTGCGCCCGGACACCATCCTGGCCTCGGTGATGTCGGTCAACAACGAGATCGGCGTG
ATCCAGGACATCGAGCAGATCGGCGAGATCTGCCGCGACAAGGGCGTCATCTTCCACGTCGACGCCGCGCAGGCGACCGG
CAAGGTGGAGCTCGACCTGCAGAAGCTGAAGGTCGACCTGATGTCCTTCTCGGCGCACAAGACGTATGGCCCGAAGGGTA
TCGGCGCGCTGTACGTGCGCCGCAAGCCGCGCATCCGCGTCGAAGCGCAGATGCACGGCGGCGGCCACGAGCGCGGCATG
CGCTCGGGCACGCTGCCGACGCACCAGATCGTCGGCATGGGCGAGGCCTTCCGCATCGCGCGTGAAGAAATGGCCACCGA
GAACGAGCGCATCCGCATGCTGCGCGATCGCCTGCACCGCGGCCTGACCGCAATGGAAGAGGTCTACGTCAACGGCGACA
TGGAGCGCCGCGTGCCGCACAACCTCAACATCAGCTTCAACTTCGTCGAAGGCGAGTCGCTGATCATGGCGATCAAGGAT
GTCGCCGTGTCGTCGGGCTCCGCGTGCACGTCGGCATCGCTGGAACCGTCCTACGTGCTGCGCGCCCTGGGCCGCAACGA
CGAACTGGCCCACAGTTCGATCCGCTTCACGGTCGGCCGCTTCACCACCGAGCAGGAAGTCGACTACGTGGTCGAGCTGC
TCAAGAGCAAGATCGGCAAGCTGCGCGACCTGTCCCCGCTGTGGGAGATGTACAAGGACGGCGTGGATCTGAACAGCATT
CAATGGGCGGCGCACTGA

Upstream 100 bases:

>100_bases
GCCTGGCGCAATCATGAGTACGATCGCGACGGCATGACGACCGGGCGCCACGGCGCCGGTCCATACGAGAATATCGCCCC
CACACAAGGCAGATCCGATA

Downstream 100 bases:

>100_bases
GCGCGGCGTTTGACGGGTAACGGCGCACCGCGCCAGCCAACGATTGAAAGAGGTGTGAAATGTCTTACAGCAGCAAGGTT
CTGGACCACTACGAAAACCC

Product: pyridoxal-phosphate-dependent aminotransferase (NIFS protein)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSTPHLPIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVAALVNADPREIVWTSGATESD
NLALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGFEVTYLDVQENGLIDIEQFKQALRPDTILASVMSVNNEIGV
IQDIEQIGEICRDKGVIFHVDAAQATGKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGM
RSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNGDMERRVPHNLNISFNFVEGESLIMAIKD
VAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSI
QWAAH

Sequences:

>Translated_405_residues
MSTPHLPIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVAALVNADPREIVWTSGATESD
NLALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGFEVTYLDVQENGLIDIEQFKQALRPDTILASVMSVNNEIGV
IQDIEQIGEICRDKGVIFHVDAAQATGKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGM
RSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNGDMERRVPHNLNISFNFVEGESLIMAIKD
VAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSI
QWAAH
>Mature_404_residues
STPHLPIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVAALVNADPREIVWTSGATESDN
LALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGFEVTYLDVQENGLIDIEQFKQALRPDTILASVMSVNNEIGVI
QDIEQIGEICRDKGVIFHVDAAQATGKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGMR
SGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNGDMERRVPHNLNISFNFVEGESLIMAIKDV
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGRFTTEQEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSIQ
WAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=60, Blast_Score=528, Evalue=1e-150,
Organism=Homo sapiens, GI156713448, Length=420, Percent_Identity=31.6666666666667, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=74.3781094527363, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1789175, Length=213, Percent_Identity=31.4553990610329, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787970, Length=395, Percent_Identity=24.5569620253165, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=59.1022443890274, Blast_Score=511, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.1100917431193, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=56.6416040100251, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=57.5, Blast_Score=499, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45266; Mature: 45135

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPHLPIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVA
CCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ALVNADPREIVWTSGATESDNLALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGF
HHHCCCCCCEEEECCCCCCCCEEEEECHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
EVTYLDVQENGLIDIEQFKQALRPDTILASVMSVNNEIGVIQDIEQIGEICRDKGVIFHV
EEEEEEECCCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEE
DAAQATGKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGM
ECCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNGDMERRVPH
CCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
NLNISFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGR
CCCEEEEEECCCEEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEEC
FTTEQEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSIQWAAH
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure 
STPHLPIYMDYSATTPVDPRVVDKMIPYLREQFGNPASRSHAFGWEAEKAVETAREQVA
CCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
ALVNADPREIVWTSGATESDNLALKGAANFYGSKGKHIITVKTEHKAVLDTARELERQGF
HHHCCCCCCEEEECCCCCCCCEEEEECHHCCCCCCCEEEEEECCCHHHHHHHHHHHHCCC
EVTYLDVQENGLIDIEQFKQALRPDTILASVMSVNNEIGVIQDIEQIGEICRDKGVIFHV
EEEEEEECCCCCEEHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCEEEEE
DAAQATGKVELDLQKLKVDLMSFSAHKTYGPKGIGALYVRRKPRIRVEAQMHGGGHERGM
ECCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLPTHQIVGMGEAFRIAREEMATENERIRMLRDRLHRGLTAMEEVYVNGDMERRVPH
CCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCC
NLNISFNFVEGESLIMAIKDVAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTVGR
CCCEEEEEECCCEEEEEEHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCEEEEEEC
FTTEQEVDYVVELLKSKIGKLRDLSPLWEMYKDGVDLNSIQWAAH
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA