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The map label for this gene is rfaD

Identifier: 17545634

GI number: 17545634

Start: 966945

End: 967940

Strand: Direct

Name: rfaD

Synonym: RSc0915

Alternate gene names: 17545634

Gene position: 966945-967940 (Clockwise)

Preceding gene: 17545633

Following gene: 17545635

Centisome position: 26.02

GC content: 64.56

Gene sequence:

>996_bases
ATGACCATCATCGTGACCGGCGCGGCCGGCTTCATCGGCGCCAACCTCGTCAAGGGCCTCAATGACCGCGGCGAGACCGA
CATCATCGCGGTCGACAACCTGACCCGCGCCGACAAGTTCAGGAACCTGGTCGACTGCGAGATCAGCGACTACCTGGACA
AGACCGAGTTCGTCGAGCGCTTTGCGCGCGGCGACTTCGGCAAGGTCCGCGCGATCTTCCACGAGGGCGCCTGCTCCGAC
ACCATGGAGACCGACGGCCGCTACATGATGGAGAACAACTACCGGTACACGCTCGCGCTGATGAAGGCCTGCCTGGACCA
GGGCGTGCAGTTCCTCTACGCGTCGTCGGCGGCCACCTACGGCGCGTCGGACACCTTCCGCGAGGACCGCGAGTTCGAGA
AGCCGCTCAACGTGTACGGCTACTCCAAGTTCCTGTTCGACCAGATCGTGCGCCGCACGCTGCCGGGCGCGCTGTCGCAG
ATCGTTGGCTTCCGCTATTTCAACGTCTACGGCCCGCGCGAGCAGCACAAGGGGCGCATGGCGTCGGTGGCCTTCCACAA
CTTCAACCAGTTCCGCACGGAAGGCACCGTCAAGCTGTTCGGCGAGTACAACGGCTATCCGCAGGGTGGCCAGATGCGCG
ACTTCGTGTCGGTGGAGGACGTGGTCAAGGTCAACCTGTTCTTCTTCGACCATCCGGACAAGTCGGGCATCTTCAACCTC
GGTACCGGCCGCGCCCAGCCGTTCAACGACATCGCGACCACCGTGGTGAACACCCTGCGCGAGGCCGAAGGCAAACCGGC
GCTCTCGACCGAAGAGTTGGCGCAGGAAGGCCTGGTCGAATACGTGAAATTTCCCGATGCGCTGCGCGGCAAATACCAGT
GCTTCACCCAGGCCGACCAGGGCAAGCTGCGCGCGGCTGGCTACAGCGCGCCGTTCCTGACCGTGCAGGAAGGCGTGGCG
CGCTACTGCCGCTGGCTGATGGCCCAGCCGGCCTGA

Upstream 100 bases:

>100_bases
TCAAGGAAGCCGTGCAGTACGCCAACCGTGCGGGCGGCATCGTGGTCGGCAAGCTGGGTACCGCCGTCGTCACCTACGCA
GAACTCTTCAATCAAGCATC

Downstream 100 bases:

>100_bases
CGACCGGCGATCGGACGGTTCGTTACTTTTCGTTGGCGCGGGCCTCCCGGCCTGCTGTCCTCCCTAAGATGCATTGAAGC
CGCCACGCCGTGTGGCGGTC

Product: ADP-L-glycero-D-manno-heptose-6-epimerase

Products: NA

Alternate protein names: ADP-L-glycero-beta-D-manno-heptose-6-epimerase; ADP-glyceromanno-heptose 6-epimerase; ADP-hep 6-epimerase; AGME

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTIIVTGAAGFIGANLVKGLNDRGETDIIAVDNLTRADKFRNLVDCEISDYLDKTEFVERFARGDFGKVRAIFHEGACSD
TMETDGRYMMENNYRYTLALMKACLDQGVQFLYASSAATYGASDTFREDREFEKPLNVYGYSKFLFDQIVRRTLPGALSQ
IVGFRYFNVYGPREQHKGRMASVAFHNFNQFRTEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDHPDKSGIFNL
GTGRAQPFNDIATTVVNTLREAEGKPALSTEELAQEGLVEYVKFPDALRGKYQCFTQADQGKLRAAGYSAPFLTVQEGVA
RYCRWLMAQPA

Sequences:

>Translated_331_residues
MTIIVTGAAGFIGANLVKGLNDRGETDIIAVDNLTRADKFRNLVDCEISDYLDKTEFVERFARGDFGKVRAIFHEGACSD
TMETDGRYMMENNYRYTLALMKACLDQGVQFLYASSAATYGASDTFREDREFEKPLNVYGYSKFLFDQIVRRTLPGALSQ
IVGFRYFNVYGPREQHKGRMASVAFHNFNQFRTEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDHPDKSGIFNL
GTGRAQPFNDIATTVVNTLREAEGKPALSTEELAQEGLVEYVKFPDALRGKYQCFTQADQGKLRAAGYSAPFLTVQEGVA
RYCRWLMAQPA
>Mature_330_residues
TIIVTGAAGFIGANLVKGLNDRGETDIIAVDNLTRADKFRNLVDCEISDYLDKTEFVERFARGDFGKVRAIFHEGACSDT
METDGRYMMENNYRYTLALMKACLDQGVQFLYASSAATYGASDTFREDREFEKPLNVYGYSKFLFDQIVRRTLPGALSQI
VGFRYFNVYGPREQHKGRMASVAFHNFNQFRTEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDHPDKSGIFNLG
TGRAQPFNDIATTVVNTLREAEGKPALSTEELAQEGLVEYVKFPDALRGKYQCFTQADQGKLRAAGYSAPFLTVQEGVAR
YCRWLMAQPA

Specific function: Catalyzes the interconversion between ADP-D-glycero- beta-D-manno-heptose and ADP-L-glycero-beta-D-manno-heptose via an epimerization at carbon 6 of the heptose

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. HldD subfamily

Homologues:

Organism=Escherichia coli, GI1790049, Length=326, Percent_Identity=56.441717791411, Blast_Score=346, Evalue=1e-96,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): HLDD_RALSO (Q8Y0X8)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519036.1
- ProteinModelPortal:   Q8Y0X8
- SMR:   Q8Y0X8
- GeneID:   1219723
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0915
- NMPDR:   fig|267608.1.peg.915
- HOGENOM:   HBG755066
- OMA:   GFIGSAM
- ProtClustDB:   CLSK896740
- BioCyc:   RSOL267608:RSC0915-MONOMER
- BRENDA:   5.1.3.20
- HAMAP:   MF_01601
- InterPro:   IPR001509
- InterPro:   IPR011912
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10366:SF29
- TIGRFAMs:   TIGR02197

Pfam domain/function: PF01370 Epimerase

EC number: =5.1.3.20

Molecular weight: Translated: 37254; Mature: 37123

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: ACT_SITE 139-139 ACT_SITE 177-177 BINDING 39-39 BINDING 54-54 BINDING 92-92 BINDING 143-143 BINDING 168-168 BINDING 169-169 BINDING 177-177 BINDING 179-179 BINDING 186-186 BINDING 213-213 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIVTGAAGFIGANLVKGLNDRGETDIIAVDNLTRADKFRNLVDCEISDYLDKTEFVER
CEEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHH
FARGDFGKVRAIFHEGACSDTMETDGRYMMENNYRYTLALMKACLDQGVQFLYASSAATY
HHCCCHHHHHHHHHCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GASDTFREDREFEKPLNVYGYSKFLFDQIVRRTLPGALSQIVGFRYFNVYGPREQHKGRM
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCHHHCCCC
ASVAFHNFNQFRTEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDHPDKSGIFNL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHEEEEEEEEEECCCCCCEEEC
GTGRAQPFNDIATTVVNTLREAEGKPALSTEELAQEGLVEYVKFPDALRGKYQCFTQADQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCCCHHEECCCCC
GKLRAAGYSAPFLTVQEGVARYCRWLMAQPA
CCEEECCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIIVTGAAGFIGANLVKGLNDRGETDIIAVDNLTRADKFRNLVDCEISDYLDKTEFVER
EEEEECCCHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHH
FARGDFGKVRAIFHEGACSDTMETDGRYMMENNYRYTLALMKACLDQGVQFLYASSAATY
HHCCCHHHHHHHHHCCCCCCCHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
GASDTFREDREFEKPLNVYGYSKFLFDQIVRRTLPGALSQIVGFRYFNVYGPREQHKGRM
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCHHHCCCC
ASVAFHNFNQFRTEGTVKLFGEYNGYPQGGQMRDFVSVEDVVKVNLFFFDHPDKSGIFNL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHEEEEEEEEEECCCCCCEEEC
GTGRAQPFNDIATTVVNTLREAEGKPALSTEELAQEGLVEYVKFPDALRGKYQCFTQADQ
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCCCHHEECCCCC
GKLRAAGYSAPFLTVQEGVARYCRWLMAQPA
CCEEECCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852