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The map label for this gene is rfaE [H]

Identifier: 17545633

GI number: 17545633

Start: 965989

End: 966948

Strand: Direct

Name: rfaE [H]

Synonym: RSc0914

Alternate gene names: 17545633

Gene position: 965989-966948 (Clockwise)

Preceding gene: 17545632

Following gene: 17545634

Centisome position: 25.99

GC content: 67.6

Gene sequence:

>960_bases
TTGCCCGCAGCCATGACCAAGACCACGCTCGCTTCCGAACAGATCCATGCCGCTCGCATCCTCGTGGTGGGCGACATGAT
GCTCGACCGCTACTGGTTCGGCGATGTCGAACGGATTTCGCCCGAAGCCCCGGTGCCGGTGGTGCAGATCAAGCGCTCGG
ACGAACGGCTAGGCGGCGCCGCCAACGTGGCGCGCAACGTGGCCGCGCTGGGGGCGCAGGCTGGCATGCTGGGCGTGATC
GGCGAGGATGAGCCGGGCCGCACCATCGAGGCGCTGCTCGACGCCAGCCATGTCCGCGGCCATCTGCACCGCGATCCGAG
CGTCAACACCACCATCAAGCTGCGCGTGCTGGCACGCCAGCAGCAGCTGATCCGCGTCGATTTCGAGAATCCGCCGACGC
ACGAAGTGCTGATATCGGTGCTGGACCGCTTCGGCACGCTGCTGCCCGAGCACGACGTGGTGGTGCTGTCCGACTACGGC
AAGGGCGGGCTCACGCACGTCGCCAAGATGATCGAGGCGGCGCGCCAGGCCGGCCGCCGCGTGCTGGTCGATCCGAAGGG
CGACGACTACTCGCGCTACAAGGGCGCGACCATGATCACGCCCAACCGCGCCGAGATGCGGCAGGTGGTCGGCAGCTGGA
AGACCGAGGCCGACCTGACCATCCGCGCGCAGAACCTGCGCCGCGAACTGCAGCTCGAGGCGCTGCTGCTGACGCGCTCG
GAAGAGGGCATGACGCTCTACACCGAGCATGAGGTGCTGCATGTGTCGGCCCAGGCGCGCGAGGTGTACGACGTGTCCGG
CGCAGGCGACACCGTCATCGCCACGCTGGCGACGCTGCTGGGCGCCGGCGCCAACATCAAGGAAGCCGTGCAGTACGCCA
ACCGTGCGGGCGGCATCGTGGTCGGCAAGCTGGGTACCGCCGTCGTCACCTACGCAGAACTCTTCAATCAAGCATCATGA

Upstream 100 bases:

>100_bases
GCAATCTGTTCGAGCCGCAAGCCATGCGCGACGCGGGGTTCGAATACCAGGCCATCGGGCGCTCCACCCAATCGCCTTCG
CAGTAACCGGAGGGCCGCTT

Downstream 100 bases:

>100_bases
CCATCATCGTGACCGGCGCGGCCGGCTTCATCGGCGCCAACCTCGTCAAGGGCCTCAATGACCGCGGCGAGACCGACATC
ATCGCGGTCGACAACCTGAC

Product: ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MPAAMTKTTLASEQIHAARILVVGDMMLDRYWFGDVERISPEAPVPVVQIKRSDERLGGAANVARNVAALGAQAGMLGVI
GEDEPGRTIEALLDASHVRGHLHRDPSVNTTIKLRVLARQQQLIRVDFENPPTHEVLISVLDRFGTLLPEHDVVVLSDYG
KGGLTHVAKMIEAARQAGRRVLVDPKGDDYSRYKGATMITPNRAEMRQVVGSWKTEADLTIRAQNLRRELQLEALLLTRS
EEGMTLYTEHEVLHVSAQAREVYDVSGAGDTVIATLATLLGAGANIKEAVQYANRAGGIVVGKLGTAVVTYAELFNQAS

Sequences:

>Translated_319_residues
MPAAMTKTTLASEQIHAARILVVGDMMLDRYWFGDVERISPEAPVPVVQIKRSDERLGGAANVARNVAALGAQAGMLGVI
GEDEPGRTIEALLDASHVRGHLHRDPSVNTTIKLRVLARQQQLIRVDFENPPTHEVLISVLDRFGTLLPEHDVVVLSDYG
KGGLTHVAKMIEAARQAGRRVLVDPKGDDYSRYKGATMITPNRAEMRQVVGSWKTEADLTIRAQNLRRELQLEALLLTRS
EEGMTLYTEHEVLHVSAQAREVYDVSGAGDTVIATLATLLGAGANIKEAVQYANRAGGIVVGKLGTAVVTYAELFNQAS
>Mature_318_residues
PAAMTKTTLASEQIHAARILVVGDMMLDRYWFGDVERISPEAPVPVVQIKRSDERLGGAANVARNVAALGAQAGMLGVIG
EDEPGRTIEALLDASHVRGHLHRDPSVNTTIKLRVLARQQQLIRVDFENPPTHEVLISVLDRFGTLLPEHDVVVLSDYGK
GGLTHVAKMIEAARQAGRRVLVDPKGDDYSRYKGATMITPNRAEMRQVVGSWKTEADLTIRAQNLRRELQLEALLLTRSE
EGMTLYTEHEVLHVSAQAREVYDVSGAGDTVIATLATLLGAGANIKEAVQYANRAGGIVVGKLGTAVVTYAELFNQAS

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=318, Percent_Identity=49.685534591195, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 34644; Mature: 34513

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAMTKTTLASEQIHAARILVVGDMMLDRYWFGDVERISPEAPVPVVQIKRSDERLGGA
CCCCHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHCCCCCCCCEEEEECCHHHCCCH
ANVARNVAALGAQAGMLGVIGEDEPGRTIEALLDASHVRGHLHRDPSVNTTIKLRVLARQ
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
QQLIRVDFENPPTHEVLISVLDRFGTLLPEHDVVVLSDYGKGGLTHVAKMIEAARQAGRR
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCE
VLVDPKGDDYSRYKGATMITPNRAEMRQVVGSWKTEADLTIRAQNLRRELQLEALLLTRS
EEECCCCCCHHHHCCCEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHEEEEC
EEGMTLYTEHEVLHVSAQAREVYDVSGAGDTVIATLATLLGAGANIKEAVQYANRAGGIV
CCCCEEEECCEEEEEECHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
VGKLGTAVVTYAELFNQAS
EECHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PAAMTKTTLASEQIHAARILVVGDMMLDRYWFGDVERISPEAPVPVVQIKRSDERLGGA
CCCHHHHHHHHHHHHHEEEEEEHHHHHHHHHCCCHHHCCCCCCCCEEEEECCHHHCCCH
ANVARNVAALGAQAGMLGVIGEDEPGRTIEALLDASHVRGHLHRDPSVNTTIKLRVLARQ
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECC
QQLIRVDFENPPTHEVLISVLDRFGTLLPEHDVVVLSDYGKGGLTHVAKMIEAARQAGRR
CCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCE
VLVDPKGDDYSRYKGATMITPNRAEMRQVVGSWKTEADLTIRAQNLRRELQLEALLLTRS
EEECCCCCCHHHHCCCEEECCCHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHEEEEC
EEGMTLYTEHEVLHVSAQAREVYDVSGAGDTVIATLATLLGAGANIKEAVQYANRAGGIV
CCCCEEEECCEEEEEECHHHHEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
VGKLGTAVVTYAELFNQAS
EECHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA