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The map label for this gene is aroA

Identifier: 17545626

GI number: 17545626

Start: 958441

End: 959751

Strand: Direct

Name: aroA

Synonym: RSc0907

Alternate gene names: 17545626

Gene position: 958441-959751 (Clockwise)

Preceding gene: 17545625

Following gene: 17545627

Centisome position: 25.79

GC content: 69.72

Gene sequence:

>1311_bases
ATGGAACACCTCGACGTCGGTCCGCTGAAGGCGGCGCGCGGCACGGTCAAGCTGCCGGGCTCGAAGAGCATCTCCAACCG
CGTGCTGCTGCTGGCCGCCCTCGCCGAGGGCGAGACCGTCGTGCGCGACCTGCTCGATTCCGACGACACCCGCGTGATGC
TGGCCGCGCTCGACACGCTCGGCGTGCGATGCGAGCCCCTCGGCACCGCCAACGCCTATCGCGTGACCGGCACGGGCGGT
CGTTTCCCGGCCAAGTCCGCCGATCTGTTCATGGGCAACGCCGGCACCGCCATCCGCCCGCTGACGGCGGCCCTGGCGCT
GCAGGGCGGCGAGTACACGCTGCACGGCGTGCCGCGCATGCACGAGCGGCCGATCGGCGATCTGGTCGACGGCCTGCGGC
AAGTCGGCGCGCGCATCGACTACACCGGCAATGAAGGTTTCCCGCCGCTGGCGATCCGCGCGGCCTCCATCCGGATCGAC
GCGCCGATCCGCGTGCGCGGCGATGTGTCGAGCCAGTTCCTGACCGCGCTACTGATGGCGCTGCCGCTGGTGGAGGGGAG
CGGTCGGCCGGTGACCATCGAGGTGGTCGGCGAGCTGATCTCGAAGCCGTACATCGAAATCACGCTCAACCTGATGGCGC
GCTTCGGAGTACAGGTCGAGCGCAACGGCTGGGCGTCGTTCAGCGTGCCGACCGGCGTGGCTTACCGTGCGCCGGGCGAG
ATCTTTGTCGAGGGCGACGCGTCGTCCGCGTCGTACTTCCTGGCGGCGGGCGCGCTCGGCGGCGGGCCGGTGCGCGTGGA
AGGGGTCGGCATGTCGAGCATCCAGGGCGACGTGCGCTTTGCCGATGCGCTCAATCGCATGGGCGCCAACGTCATGGCCG
GCGACAACTGGATCGAAGTGCGGGGCGTGGAGCGCGACGACGGCAAGCTGCATGCGCTGGAGCTCGACTGCAATCACATC
CCCGATGCCGCCATGACGCTGGCGGTGGCCGCGCTGTTCGCCGATGGCACCACCACGCTGACCAACATCGGCAGTTGGCG
CGTCAAGGAGACCGACCGGCTGACCGCGATGGCCACCGAGCTGCGCAAGCTTGGCGCCGCGGTGGAAGAGGGCACCGACT
ACATCCGCGTGACCCCGCCGTCGCACTGGACCGCGCCTGCCGGCGGGATCGACACCTACGACGATCACCGCATGGCGATG
GCCTTTTCGCTGGCGGCCTTCGGCCCGGTGCCGGTACGCATCAATGACCCGCGCTGCGTGGCCAAGACGTTCCCCGAATA
CTTCACGGCGTTCGGCGGAATCGCCGCCTGA

Upstream 100 bases:

>100_bases
CGAGCGCGTGTTCCGCCGCGCCAGCGAAGCGCGCCTGGCCTGGCCGCAGCGCGCGGCCTCCGCCACACAACCCGAATAAC
GTTTCTCTCTGACTGGATCC

Downstream 100 bases:

>100_bases
AACGCAATTAACCGCCAATCATCATGTCTGACGTTGCTGCTTTTACCGCGTACCCGGTCATCGCCATCGACGGTCCGACC
GCGTCCGGCAAGGGCACGGT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MEHLDVGPLKAARGTVKLPGSKSISNRVLLLAALAEGETVVRDLLDSDDTRVMLAALDTLGVRCEPLGTANAYRVTGTGG
RFPAKSADLFMGNAGTAIRPLTAALALQGGEYTLHGVPRMHERPIGDLVDGLRQVGARIDYTGNEGFPPLAIRAASIRID
APIRVRGDVSSQFLTALLMALPLVEGSGRPVTIEVVGELISKPYIEITLNLMARFGVQVERNGWASFSVPTGVAYRAPGE
IFVEGDASSASYFLAAGALGGGPVRVEGVGMSSIQGDVRFADALNRMGANVMAGDNWIEVRGVERDDGKLHALELDCNHI
PDAAMTLAVAALFADGTTTLTNIGSWRVKETDRLTAMATELRKLGAAVEEGTDYIRVTPPSHWTAPAGGIDTYDDHRMAM
AFSLAAFGPVPVRINDPRCVAKTFPEYFTAFGGIAA

Sequences:

>Translated_436_residues
MEHLDVGPLKAARGTVKLPGSKSISNRVLLLAALAEGETVVRDLLDSDDTRVMLAALDTLGVRCEPLGTANAYRVTGTGG
RFPAKSADLFMGNAGTAIRPLTAALALQGGEYTLHGVPRMHERPIGDLVDGLRQVGARIDYTGNEGFPPLAIRAASIRID
APIRVRGDVSSQFLTALLMALPLVEGSGRPVTIEVVGELISKPYIEITLNLMARFGVQVERNGWASFSVPTGVAYRAPGE
IFVEGDASSASYFLAAGALGGGPVRVEGVGMSSIQGDVRFADALNRMGANVMAGDNWIEVRGVERDDGKLHALELDCNHI
PDAAMTLAVAALFADGTTTLTNIGSWRVKETDRLTAMATELRKLGAAVEEGTDYIRVTPPSHWTAPAGGIDTYDDHRMAM
AFSLAAFGPVPVRINDPRCVAKTFPEYFTAFGGIAA
>Mature_436_residues
MEHLDVGPLKAARGTVKLPGSKSISNRVLLLAALAEGETVVRDLLDSDDTRVMLAALDTLGVRCEPLGTANAYRVTGTGG
RFPAKSADLFMGNAGTAIRPLTAALALQGGEYTLHGVPRMHERPIGDLVDGLRQVGARIDYTGNEGFPPLAIRAASIRID
APIRVRGDVSSQFLTALLMALPLVEGSGRPVTIEVVGELISKPYIEITLNLMARFGVQVERNGWASFSVPTGVAYRAPGE
IFVEGDASSASYFLAAGALGGGPVRVEGVGMSSIQGDVRFADALNRMGANVMAGDNWIEVRGVERDDGKLHALELDCNHI
PDAAMTLAVAALFADGTTTLTNIGSWRVKETDRLTAMATELRKLGAAVEEGTDYIRVTPPSHWTAPAGGIDTYDDHRMAM
AFSLAAFGPVPVRINDPRCVAKTFPEYFTAFGGIAA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=436, Percent_Identity=55.045871559633, Blast_Score=435, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320332, Length=449, Percent_Identity=39.4209354120267, Blast_Score=246, Evalue=5e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_RALSO (Q8Y0Y6)

Other databases:

- EMBL:   AL646052
- RefSeq:   NP_519028.1
- ProteinModelPortal:   Q8Y0Y6
- SMR:   Q8Y0Y6
- GeneID:   1219715
- GenomeReviews:   AL646052_GR
- KEGG:   rso:RSc0907
- NMPDR:   fig|267608.1.peg.907
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- BioCyc:   RSOL267608:RSC0907-MONOMER
- BRENDA:   2.5.1.19
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46145; Mature: 46145

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLDVGPLKAARGTVKLPGSKSISNRVLLLAALAEGETVVRDLLDSDDTRVMLAALDTL
CCCCCCCCCHHCCCEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEHHHC
GVRCEPLGTANAYRVTGTGGRFPAKSADLFMGNAGTAIRPLTAALALQGGEYTLHGVPRM
CCEECCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHEEECCCCEEEECCCCH
HERPIGDLVDGLRQVGARIDYTGNEGFPPLAIRAASIRIDAPIRVRGDVSSQFLTALLMA
HCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCCEEEECCCHHHHHHHHHHH
LPLVEGSGRPVTIEVVGELISKPYIEITLNLMARFGVQVERNGWASFSVPTGVAYRAPGE
HHHCCCCCCEEEHHHHHHHHCCCEEEEEHEEHHHCCCEEECCCCEEECCCCCEEEECCCC
IFVEGDASSASYFLAAGALGGGPVRVEGVGMSSIQGDVRFADALNRMGANVMAGDNWIEV
EEEECCCCCCEEEEEECCCCCCCEEEEECCHHHCCCCHHHHHHHHHCCCEEEECCCEEEE
RGVERDDGKLHALELDCNHIPDAAMTLAVAALFADGTTTLTNIGSWRVKETDRLTAMATE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEECCCHHHHHHHHH
LRKLGAAVEEGTDYIRVTPPSHWTAPAGGIDTYDDHRMAMAFSLAAFGPVPVRINDPRCV
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHEEEEEHHHCCCEEEEECCCCHH
AKTFPEYFTAFGGIAA
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEHLDVGPLKAARGTVKLPGSKSISNRVLLLAALAEGETVVRDLLDSDDTRVMLAALDTL
CCCCCCCCCHHCCCEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCCCCCEEEEEEHHHC
GVRCEPLGTANAYRVTGTGGRFPAKSADLFMGNAGTAIRPLTAALALQGGEYTLHGVPRM
CCEECCCCCCCEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHEEECCCCEEEECCCCH
HERPIGDLVDGLRQVGARIDYTGNEGFPPLAIRAASIRIDAPIRVRGDVSSQFLTALLMA
HCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCCEEEECCCHHHHHHHHHHH
LPLVEGSGRPVTIEVVGELISKPYIEITLNLMARFGVQVERNGWASFSVPTGVAYRAPGE
HHHCCCCCCEEEHHHHHHHHCCCEEEEEHEEHHHCCCEEECCCCEEECCCCCEEEECCCC
IFVEGDASSASYFLAAGALGGGPVRVEGVGMSSIQGDVRFADALNRMGANVMAGDNWIEV
EEEECCCCCCEEEEEECCCCCCCEEEEECCHHHCCCCHHHHHHHHHCCCEEEECCCEEEE
RGVERDDGKLHALELDCNHIPDAAMTLAVAALFADGTTTLTNIGSWRVKETDRLTAMATE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCEEECCCHHHHHHHHH
LRKLGAAVEEGTDYIRVTPPSHWTAPAGGIDTYDDHRMAMAFSLAAFGPVPVRINDPRCV
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCHHEEEEEHHHCCCEEEEECCCCHH
AKTFPEYFTAFGGIAA
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852