Click here to switch to the map view.

The map label for this gene is cisA [H]

Identifier: 17545610

GI number: 17545610

Start: 937582

End: 938964

Strand: Direct

Name: cisA [H]

Synonym: RSc0891

Alternate gene names: 17545610

Gene position: 937582-938964 (Clockwise)

Preceding gene: 17545609

Following gene: 17545611

Centisome position: 25.23

GC content: 64.71

Gene sequence:

>1383_bases
ATGAGCCTCGAAGCAAGCAGGAAGCGCCGGCGCTGCGCGGTCTACGCCCGTAAGTCCTCGGACGAGGGGCTCGACCAGGA
CTTCAACTCGATCGATGCGCAGCGTGACGCGGGTCACGCCTACATCGCCAGCCAGCGCGCCGAGGGCTGGATTCCGGTGG
CGGACGACTACGACGATCCCGCGTACTCCGGCGGCAACATGGAGCGGCCGGGCCTGAAGCGTTTGCTGGCGGACATCGAG
CGAGGCTTGGTCGATATCGTCGTGGTCTACAAGATCGACCGGCTGACCCGCAGCCTGGCGGACTTCTCCAAGATGGTGGA
GGTGTTCGAACGCCACGATGTGTCCTTCGTGTCGGTCACCCAGCAGTTCAACACGACCACGTCGATGGGGCGGCTGATGC
TGAATGTGCTGCTGTCCTTCGCGCAATTCGAGCGCGAGGTGACGGGCGAACGTATCCGCGACAAGATCGCGGCGAGCAAA
CGCAAGGGCATGTGGATGGGCGGCGTGCCCCCGCTGGGCTATGACGTGCTCCACCGTAAGCTGATTGTCAACGAGGCGGA
GGCCACGGTGGTGCGCCGCCTGTTCGCGGAGTTTCCGCGCGCTGCGTCCACGACACTGTTGGTGCAGCGGCTGCGTCACG
AAGGTGTGATGACCAAGTCGTGGGTCGCGCAGTCTGGCAACGACCGGATGGGCAAGCTGATGGACAAGGGCGCGCTGTAC
AAGATTCTGAGCAATCCGGTCTACCTGGGCCAGATCCGACACAAGGAGATCAGCTACGCCGGCGAGCACGAGCCTATCGT
GACGCAGGAGCAATGGGATGCCGTGCAGTCGGCGCTGACCAGCAAGCCCACCGGCGTGAGCCGCGGCCAGATCCGTACTG
AACGTCCGGCGCTGCTCAAGGGACTGATTTGCACGGCTGACGGCCGGGCCATGACGCCGCATACAACCAAACGGCGGGGT
GGGCGGTTGTATCGGTACTACTTGTCGACACGGGATGCCCAGGAGGGATACGGCGCGTCCGACGTCAGAATGCTGCCGGC
GGGCGAGGTCGAGGAGGCCGTGGTGGCGCAGTTGCGCGGCATCTTGCGCGCGCCGGAAATGGTCGCCCGGGTTTGGCGGG
AAGTGGCCAGAAGCAACGATACCCACGACATGACCGAAATGCAGGTGGCCGTGGCACTGTCACGCATCGACATCGTGTGG
GAGAACCTGTTCCCGCTGGAGCAGCACCGCATCGTGCAACTGCTGGTCGAGCGTGTCGTCGTGTCGCCCCAGGAACTGCG
GGTGCAGTTGCATCGCAACGGCATCGAGCATTTTGCGCTGGACGTGGTGCGTGCCGCCGGTGGGAAATCCGTGCCCGCTG
CCGCAGGGGAGGCCCTGGCATGA

Upstream 100 bases:

>100_bases
TCACGGCGATCGCCTGCGAAATCGCGGGCACGCGCTGGTCGGGGCCGGCGTTCTTCGGTCTGCGGGATGGCACGAAGAAA
CAGCGCAAGGGGACCGGTGC

Downstream 100 bases:

>100_bases
GGCGCACGACCCTGGAATTTCCTGGGGAGCCGATGGTGTTGCATACGGGCAACGGCGGTATCGAGGTGTCCATCCCGATC
CGCGTGCGCCGCTACAGCGG

Product: site-specific integrase/recombinase

Products: NA

Alternate protein names: Stage IV sporulation protein CA [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSLEASRKRRRCAVYARKSSDEGLDQDFNSIDAQRDAGHAYIASQRAEGWIPVADDYDDPAYSGGNMERPGLKRLLADIE
RGLVDIVVVYKIDRLTRSLADFSKMVEVFERHDVSFVSVTQQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKIAASK
RKGMWMGGVPPLGYDVLHRKLIVNEAEATVVRRLFAEFPRAASTTLLVQRLRHEGVMTKSWVAQSGNDRMGKLMDKGALY
KILSNPVYLGQIRHKEISYAGEHEPIVTQEQWDAVQSALTSKPTGVSRGQIRTERPALLKGLICTADGRAMTPHTTKRRG
GRLYRYYLSTRDAQEGYGASDVRMLPAGEVEEAVVAQLRGILRAPEMVARVWREVARSNDTHDMTEMQVAVALSRIDIVW
ENLFPLEQHRIVQLLVERVVVSPQELRVQLHRNGIEHFALDVVRAAGGKSVPAAAGEALA

Sequences:

>Translated_460_residues
MSLEASRKRRRCAVYARKSSDEGLDQDFNSIDAQRDAGHAYIASQRAEGWIPVADDYDDPAYSGGNMERPGLKRLLADIE
RGLVDIVVVYKIDRLTRSLADFSKMVEVFERHDVSFVSVTQQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKIAASK
RKGMWMGGVPPLGYDVLHRKLIVNEAEATVVRRLFAEFPRAASTTLLVQRLRHEGVMTKSWVAQSGNDRMGKLMDKGALY
KILSNPVYLGQIRHKEISYAGEHEPIVTQEQWDAVQSALTSKPTGVSRGQIRTERPALLKGLICTADGRAMTPHTTKRRG
GRLYRYYLSTRDAQEGYGASDVRMLPAGEVEEAVVAQLRGILRAPEMVARVWREVARSNDTHDMTEMQVAVALSRIDIVW
ENLFPLEQHRIVQLLVERVVVSPQELRVQLHRNGIEHFALDVVRAAGGKSVPAAAGEALA
>Mature_459_residues
SLEASRKRRRCAVYARKSSDEGLDQDFNSIDAQRDAGHAYIASQRAEGWIPVADDYDDPAYSGGNMERPGLKRLLADIER
GLVDIVVVYKIDRLTRSLADFSKMVEVFERHDVSFVSVTQQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKIAASKR
KGMWMGGVPPLGYDVLHRKLIVNEAEATVVRRLFAEFPRAASTTLLVQRLRHEGVMTKSWVAQSGNDRMGKLMDKGALYK
ILSNPVYLGQIRHKEISYAGEHEPIVTQEQWDAVQSALTSKPTGVSRGQIRTERPALLKGLICTADGRAMTPHTTKRRGG
RLYRYYLSTRDAQEGYGASDVRMLPAGEVEEAVVAQLRGILRAPEMVARVWREVARSNDTHDMTEMQVAVALSRIDIVWE
NLFPLEQHRIVQLLVERVVVSPQELRVQLHRNGIEHFALDVVRAAGGKSVPAAAGEALA

Specific function: Putative site-specific recombinase having a very important role in sporulation. It probably plays a role in the recombination of spoIIIC and spoIVCB to form sigma K factor [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 recombinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=110, Percent_Identity=37.2727272727273, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011109
- InterPro:   IPR006118
- InterPro:   IPR006119 [H]

Pfam domain/function: PF07508 Recombinase; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 51567; Mature: 51436

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLEASRKRRRCAVYARKSSDEGLDQDFNSIDAQRDAGHAYIASQRAEGWIPVADDYDDP
CCCHHHHHHHHHHEEECCCCCCCCHHHHHHCCHHCCCCHHEECCCCCCCCCCCCCCCCC
AYSGGNMERPGLKRLLADIERGLVDIVVVYKIDRLTRSLADFSKMVEVFERHDVSFVSVT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHH
QQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKIAASKRKGMWMGGVPPLGYDVLHRK
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LIVNEAEATVVRRLFAEFPRAASTTLLVQRLRHEGVMTKSWVAQSGNDRMGKLMDKGALY
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHH
KILSNPVYLGQIRHKEISYAGEHEPIVTQEQWDAVQSALTSKPTGVSRGQIRTERPALLK
HHHCCCEEEECHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
GLICTADGRAMTPHTTKRRGGRLYRYYLSTRDAQEGYGASDVRMLPAGEVEEAVVAQLRG
HHEEECCCCCCCCCCHHHCCCCEEEEEECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHH
ILRAPEMVARVWREVARSNDTHDMTEMQVAVALSRIDIVWENLFPLEQHRIVQLLVERVV
HHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
VSPQELRVQLHRNGIEHFALDVVRAAGGKSVPAAAGEALA
CCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105293; 7704261; 8969508; 9384377 [H]