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The map label for this gene is paaF [C]

Identifier: 17545195

GI number: 17545195

Start: 511093

End: 511857

Strand: Direct

Name: paaF [C]

Synonym: RSc0476

Alternate gene names: 17545195

Gene position: 511093-511857 (Clockwise)

Preceding gene: 17545194

Following gene: 17545197

Centisome position: 13.75

GC content: 70.46

Gene sequence:

>765_bases
ATGTCGATTCGTACCGAGACCGCAGACGGCATCCTCACGCTGACCTTCGACCGCCTGGACAAGAAGAACGCCATCACCGC
CGCGATGTACCAGGCGCTGGCGGATGCGCTGGTGGCCGCCGAGGACGATCCCGCCGTGCGCGTGATCGTGCTGGCCGGGC
ACGAGAGCGCCTTCACGGCCGGCAACGATCTCGAAGATTTCATGAAGCACCCGCCCAAGGACGAGCACGCGCCCGTGCAC
CGGTTCCTCAGGGCGATCAGCGCGGCGACCAAGCCGCTGGTCGCGTCGGTGAGCGGCGTGGCGGTCGGGGTGGGCACCAC
CATGCTGCTGCACTGCGACCTGGTGTACGCCTCGGACACCGCCAGGCTGTCGATGCCGTTCGCGCAGCTGGGGCTGTGTC
CCGAGGCCGCGTCGAGCCTGCTGCTGCCGCAGCTGGCGGGCTACCACCGCGCGGCCGAGAAACTGCTGTTCGGCGAGCCC
TTCGACGCCGGCGAGGCGCGCGAGCTGGGGCTGGTCAACCGCGTGCTGCCGGTGGCCGAGCTGGAGGCCTTCGTGCGCGC
CCAGGCGCGCAAGCTGACGCTGCTGCCGCCGTCGTCGCTGCGTGCGACCAAGCGGCTGATGAAGGAGGGCGCCACGCCGC
AGATCGCGGCGCGCATGTCGCTGGAGGGCCGGCAGTTCGGCGAGATGCTGCGCGCGCCCGAGGCACGCGAGGCGTTCACG
GCGTTCTTCGAGAAGCGCCGCCCGGATTTCTCCAGGTTCAGCTGA

Upstream 100 bases:

>100_bases
CGAGGGGACGGCCCAGGGGCCAACGCGTTCTCCGTCGGCGATCTGTCCGATTCGCCCGTGCCGGTGTGATCCGGGCGCGG
GCATTCTTTCTGGAGCATTC

Downstream 100 bases:

>100_bases
CCGGGCCTGGGCCCGCCGCGGCAAAAGAAAACCCGGCCGTGGCCGGGTCGTTTTGCGGGGCGTTGCCGGTTCAGCGCGGC
GGCAGCGCGCGCGGTTTGCG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSIRTETADGILTLTFDRLDKKNAITAAMYQALADALVAAEDDPAVRVIVLAGHESAFTAGNDLEDFMKHPPKDEHAPVH
RFLRAISAATKPLVASVSGVAVGVGTTMLLHCDLVYASDTARLSMPFAQLGLCPEAASSLLLPQLAGYHRAAEKLLFGEP
FDAGEARELGLVNRVLPVAELEAFVRAQARKLTLLPPSSLRATKRLMKEGATPQIAARMSLEGRQFGEMLRAPEAREAFT
AFFEKRRPDFSRFS

Sequences:

>Translated_254_residues
MSIRTETADGILTLTFDRLDKKNAITAAMYQALADALVAAEDDPAVRVIVLAGHESAFTAGNDLEDFMKHPPKDEHAPVH
RFLRAISAATKPLVASVSGVAVGVGTTMLLHCDLVYASDTARLSMPFAQLGLCPEAASSLLLPQLAGYHRAAEKLLFGEP
FDAGEARELGLVNRVLPVAELEAFVRAQARKLTLLPPSSLRATKRLMKEGATPQIAARMSLEGRQFGEMLRAPEAREAFT
AFFEKRRPDFSRFS
>Mature_253_residues
SIRTETADGILTLTFDRLDKKNAITAAMYQALADALVAAEDDPAVRVIVLAGHESAFTAGNDLEDFMKHPPKDEHAPVHR
FLRAISAATKPLVASVSGVAVGVGTTMLLHCDLVYASDTARLSMPFAQLGLCPEAASSLLLPQLAGYHRAAEKLLFGEPF
DAGEARELGLVNRVLPVAELEAFVRAQARKLTLLPPSSLRATKRLMKEGATPQIAARMSLEGRQFGEMLRAPEAREAFTA
FFEKRRPDFSRFS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI260274832, Length=247, Percent_Identity=33.1983805668016, Blast_Score=136, Evalue=1e-32,
Organism=Homo sapiens, GI45643119, Length=210, Percent_Identity=36.6666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI260275230, Length=210, Percent_Identity=36.6666666666667, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI194097323, Length=248, Percent_Identity=28.6290322580645, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=27.6, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI25453481, Length=250, Percent_Identity=25.2, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538573, Length=250, Percent_Identity=25.2, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4757966, Length=214, Percent_Identity=28.0373831775701, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI51538581, Length=214, Percent_Identity=28.0373831775701, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI4757968, Length=250, Percent_Identity=24.8, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI49355787, Length=250, Percent_Identity=24.8, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI221136756, Length=196, Percent_Identity=26.530612244898, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221136753, Length=196, Percent_Identity=26.530612244898, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI221307494, Length=196, Percent_Identity=26.530612244898, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI116235487, Length=162, Percent_Identity=30.8641975308642, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI68989263, Length=168, Percent_Identity=30.3571428571429, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI70995211, Length=196, Percent_Identity=27.0408163265306, Blast_Score=74, Evalue=9e-14,
Organism=Homo sapiens, GI4502327, Length=254, Percent_Identity=26.3779527559055, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=32.6530612244898, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=30.6513409961686, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=31.9066147859922, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI221142681, Length=245, Percent_Identity=31.4285714285714, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87082183, Length=255, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790281, Length=189, Percent_Identity=25.9259259259259, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=31.8367346938775, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17549921, Length=232, Percent_Identity=34.9137931034483, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=30.4, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=242, Percent_Identity=29.7520661157025, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=226, Percent_Identity=25.6637168141593, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=177, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=215, Percent_Identity=25.5813953488372, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=242, Percent_Identity=25.2066115702479, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24654903, Length=248, Percent_Identity=31.4516129032258, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI20129971, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24653477, Length=256, Percent_Identity=27.734375, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=28.9156626506024, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922422, Length=250, Percent_Identity=24, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24650670, Length=187, Percent_Identity=31.5508021390374, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21357171, Length=189, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=25.1366120218579, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27575; Mature: 27444

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRTETADGILTLTFDRLDKKNAITAAMYQALADALVAAEDDPAVRVIVLAGHESAFTA
CCCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
GNDLEDFMKHPPKDEHAPVHRFLRAISAATKPLVASVSGVAVGVGTTMLLHCDLVYASDT
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHEEEHEEEEEEECCC
ARLSMPFAQLGLCPEAASSLLLPQLAGYHRAAEKLLFGEPFDAGEARELGLVNRVLPVAE
CHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LEAFVRAQARKLTLLPPSSLRATKRLMKEGATPQIAARMSLEGRQFGEMLRAPEAREAFT
HHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHH
AFFEKRRPDFSRFS
HHHHHCCCCCCCCC
>Mature Secondary Structure 
SIRTETADGILTLTFDRLDKKNAITAAMYQALADALVAAEDDPAVRVIVLAGHESAFTA
CCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
GNDLEDFMKHPPKDEHAPVHRFLRAISAATKPLVASVSGVAVGVGTTMLLHCDLVYASDT
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEHHHHEEEHEEEEEEECCC
ARLSMPFAQLGLCPEAASSLLLPQLAGYHRAAEKLLFGEPFDAGEARELGLVNRVLPVAE
CHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
LEAFVRAQARKLTLLPPSSLRATKRLMKEGATPQIAARMSLEGRQFGEMLRAPEAREAFT
HHHHHHHHHCEEEECCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHH
AFFEKRRPDFSRFS
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA