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The map label for this gene is phrA [H]

Identifier: 17230217

GI number: 17230217

Start: 3320837

End: 3322276

Strand: Direct

Name: phrA [H]

Synonym: alr2725

Alternate gene names: 17230217

Gene position: 3320837-3322276 (Clockwise)

Preceding gene: 17230215

Following gene: 17230218

Centisome position: 51.78

GC content: 44.17

Gene sequence:

>1440_bases
ATGTCTGACTTAGTTTTATTTTGGCATCGCCGTGATTTACGTATTGCTGACAATATAGGGCTAGCTGCGGCTAGACAACA
AAGTTCTAAAGTCGTGGGGGTATTTTGTCTAGACCCTAATATTTTAGAAAGAGATGATGTAGCCCCTGCCAGAGTTACTT
ACATGATTGGTAGTTTACAAGAGTTGCAACAGCGATATGTGCAAGCAGGAAGCCAATTATTAATCCTGCATGGTGACCCA
GTGCAAGCTATCCCTCATTTAGCAGAGGCATTGAACGCCAAAGCTGTGTTTTGGAATTGGGATGTGGAACCATATTCACA
AACACGCGATCGCGCAATTCTTGATATCCTAAAAGATAAAGGTATCCAATTTTTAACCCACAAGTGGGATCAAATACTCC
ATTCCCCAGACGAAATCCGTTCAGGTAGCAACACACCTTACACTGTTTACACTCCCTTTTGGAAAAATTGGAGTAGCAAA
CCCAAAGCCAAACCAGTAGAAACCCTGCAAAACGCCGAAGGACTAACAGAATCTGAACAGGAACTTGCTCAAAAAGCTGG
AGTGAAAACCTTACCCACGGCGAAAGATTTAGGATTTTTCTGGGATGCAGACTTGATAATTGCCCCAGGGGAAGCAGCAG
CGCAAGCCAAGTTAGAAGAATTTACAGCCAAAGCCATCACCGAATACCAAGAACAGCGTAATTTTCCCGCCGTTGATGGC
ACATCACAACTAAGTGCAGCCTTAAAATTTGGTGTCATTGGCATTCGCACTGTTTGGCAAGCAACTTTAGAAGCGTTAGA
AAATAGCCGCAGCGAAGAAGCATCCGCAGCTATCCGCACATGGCAACAAGAATTAGCCTGGCGAGAATTTTATCAACACG
CCATGTATAACTTCCCGGAATTAGCTGATGGTGCTTACCGCGAAACCTTCAAGAATTTTCCTTGGGAAACCAACGAAGAA
CATTTTCAGGCTTGGCGTGAAGGAAAGACAGGTTATCCCATCGTAGATGCAGCCATGCGCCAGATGAACGAAAGCGGCTG
GATGCACAACCGTTGTCGAATGATTGTAGCCAGTTTCCTGACCAAAGACTTATTAATTAATCCCCAATTGGGAGAAAAAT
ACTTTATGCAGAAGCTGATTGATGGTGATTTATCTGCCAACAACGGCGGCTGGCAATGGAGTGCTTCTAGCGGGATGGAC
CCGAAGCCGTTACGAATTTTTAACCCCGCCAGCCAATCACAAAAATTTGACCCAGATGCAGAATATATTCGTCGGTGGGT
ACCTGAATTGAGGTCAATGGATACGGAGTATCTAGTAACGGGTAAGATTACACCTCTTGAGCGTCATGCTGTGGGTTATG
TTAAACCAATCGTGGATCATCAAAAACAGCAGCAACTCTTTAAACAACGTTATCAACAACAAAAAGCCACCAGTCTTTAA

Upstream 100 bases:

>100_bases
CAATCCCCAATCCCCATTTCTCAAGAAATTTGTCAGCAGAATGATGTTCAATAATACTACTAGGTCTAATCTAGTACAAG
CGAACTATAAAAATTAGAAA

Downstream 100 bases:

>100_bases
TCTTTTAACGCTAGAGTACTTCACCAGTTGAACTACTGTTTGGAAGTCCTACATAGTTCGAGCAGCAATAATAAATTTCA
GCAAAGGGGCTAATAGCTCC

Product: deoxyribopyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSDLVLFWHRRDLRIADNIGLAAARQQSSKVVGVFCLDPNILERDDVAPARVTYMIGSLQELQQRYVQAGSQLLILHGDP
VQAIPHLAEALNAKAVFWNWDVEPYSQTRDRAILDILKDKGIQFLTHKWDQILHSPDEIRSGSNTPYTVYTPFWKNWSSK
PKAKPVETLQNAEGLTESEQELAQKAGVKTLPTAKDLGFFWDADLIIAPGEAAAQAKLEEFTAKAITEYQEQRNFPAVDG
TSQLSAALKFGVIGIRTVWQATLEALENSRSEEASAAIRTWQQELAWREFYQHAMYNFPELADGAYRETFKNFPWETNEE
HFQAWREGKTGYPIVDAAMRQMNESGWMHNRCRMIVASFLTKDLLINPQLGEKYFMQKLIDGDLSANNGGWQWSASSGMD
PKPLRIFNPASQSQKFDPDAEYIRRWVPELRSMDTEYLVTGKITPLERHAVGYVKPIVDHQKQQQLFKQRYQQQKATSL

Sequences:

>Translated_479_residues
MSDLVLFWHRRDLRIADNIGLAAARQQSSKVVGVFCLDPNILERDDVAPARVTYMIGSLQELQQRYVQAGSQLLILHGDP
VQAIPHLAEALNAKAVFWNWDVEPYSQTRDRAILDILKDKGIQFLTHKWDQILHSPDEIRSGSNTPYTVYTPFWKNWSSK
PKAKPVETLQNAEGLTESEQELAQKAGVKTLPTAKDLGFFWDADLIIAPGEAAAQAKLEEFTAKAITEYQEQRNFPAVDG
TSQLSAALKFGVIGIRTVWQATLEALENSRSEEASAAIRTWQQELAWREFYQHAMYNFPELADGAYRETFKNFPWETNEE
HFQAWREGKTGYPIVDAAMRQMNESGWMHNRCRMIVASFLTKDLLINPQLGEKYFMQKLIDGDLSANNGGWQWSASSGMD
PKPLRIFNPASQSQKFDPDAEYIRRWVPELRSMDTEYLVTGKITPLERHAVGYVKPIVDHQKQQQLFKQRYQQQKATSL
>Mature_478_residues
SDLVLFWHRRDLRIADNIGLAAARQQSSKVVGVFCLDPNILERDDVAPARVTYMIGSLQELQQRYVQAGSQLLILHGDPV
QAIPHLAEALNAKAVFWNWDVEPYSQTRDRAILDILKDKGIQFLTHKWDQILHSPDEIRSGSNTPYTVYTPFWKNWSSKP
KAKPVETLQNAEGLTESEQELAQKAGVKTLPTAKDLGFFWDADLIIAPGEAAAQAKLEEFTAKAITEYQEQRNFPAVDGT
SQLSAALKFGVIGIRTVWQATLEALENSRSEEASAAIRTWQQELAWREFYQHAMYNFPELADGAYRETFKNFPWETNEEH
FQAWREGKTGYPIVDAAMRQMNESGWMHNRCRMIVASFLTKDLLINPQLGEKYFMQKLIDGDLSANNGGWQWSASSGMDP
KPLRIFNPASQSQKFDPDAEYIRRWVPELRSMDTEYLVTGKITPLERHAVGYVKPIVDHQKQQQLFKQRYQQQKATSL

Specific function: Involved in repair of UV radiation-induced DNA damage. Exhibits specific, light-dependent repair activity for a cyclobutyl pyrimidine dimer by catalyzing the light-dependent monomerization (300-600 nm) of the dimers (in cis-syn configuration), which are f

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=493, Percent_Identity=28.1947261663286, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI4758072, Length=491, Percent_Identity=28.9205702647658, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI188536103, Length=447, Percent_Identity=27.5167785234899, Blast_Score=163, Evalue=3e-40,
Organism=Escherichia coli, GI1786926, Length=471, Percent_Identity=36.3057324840764, Blast_Score=278, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324962, Length=505, Percent_Identity=28.9108910891089, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI17137248, Length=451, Percent_Identity=30.5986696230599, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24585455, Length=451, Percent_Identity=30.5986696230599, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24648152, Length=473, Percent_Identity=26.215644820296, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR019947
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54771; Mature: 54640

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLVLFWHRRDLRIADNIGLAAARQQSSKVVGVFCLDPNILERDDVAPARVTYMIGSLQ
CCCCEEEEECCCCEECCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
ELQQRYVQAGSQLLILHGDPVQAIPHLAEALNAKAVFWNWDVEPYSQTRDRAILDILKDK
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHC
GIQFLTHKWDQILHSPDEIRSGSNTPYTVYTPFWKNWSSKPKAKPVETLQNAEGLTESEQ
CHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCHHCCCCCCCCCCHHHHHHHCCCCCHHHH
ELAQKAGVKTLPTAKDLGFFWDADLIIAPGEAAAQAKLEEFTAKAITEYQEQRNFPAVDG
HHHHHCCCCCCCCHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TSQLSAALKFGVIGIRTVWQATLEALENSRSEEASAAIRTWQQELAWREFYQHAMYNFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LADGAYRETFKNFPWETNEEHFQAWREGKTGYPIVDAAMRQMNESGWMHNRCRMIVASFL
HCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
TKDLLINPQLGEKYFMQKLIDGDLSANNGGWQWSASSGMDPKPLRIFNPASQSQKFDPDA
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCH
EYIRRWVPELRSMDTEYLVTGKITPLERHAVGYVKPIVDHQKQQQLFKQRYQQQKATSL
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLVLFWHRRDLRIADNIGLAAARQQSSKVVGVFCLDPNILERDDVAPARVTYMIGSLQ
CCCEEEEECCCCEECCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
ELQQRYVQAGSQLLILHGDPVQAIPHLAEALNAKAVFWNWDVEPYSQTRDRAILDILKDK
HHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHC
GIQFLTHKWDQILHSPDEIRSGSNTPYTVYTPFWKNWSSKPKAKPVETLQNAEGLTESEQ
CHHHHHHHHHHHHCCHHHHCCCCCCCEEEECCHHCCCCCCCCCCHHHHHHHCCCCCHHHH
ELAQKAGVKTLPTAKDLGFFWDADLIIAPGEAAAQAKLEEFTAKAITEYQEQRNFPAVDG
HHHHHCCCCCCCCHHHCCEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TSQLSAALKFGVIGIRTVWQATLEALENSRSEEASAAIRTWQQELAWREFYQHAMYNFPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LADGAYRETFKNFPWETNEEHFQAWREGKTGYPIVDAAMRQMNESGWMHNRCRMIVASFL
HCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
TKDLLINPQLGEKYFMQKLIDGDLSANNGGWQWSASSGMDPKPLRIFNPASQSQKFDPDA
HHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEEECCCCCCCCCCCCH
EYIRRWVPELRSMDTEYLVTGKITPLERHAVGYVKPIVDHQKQQQLFKQRYQQQKATSL
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11254140; 8905231; 10896222; 10871367 [H]