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The map label for this gene is yfiY [H]

Identifier: 17230075

GI number: 17230075

Start: 3088246

End: 3089232

Strand: Direct

Name: yfiY [H]

Synonym: alr2583

Alternate gene names: 17230075

Gene position: 3088246-3089232 (Clockwise)

Preceding gene: 17230074

Following gene: 17230079

Centisome position: 48.15

GC content: 33.84

Gene sequence:

>987_bases
ATGTCAATTAAAAATAATTTATTTATCAGGGTATTTACCTTCCTGCACCATATACTTAATTATCATCAAATTAAGTTAAT
TTTATTCAGCATCATCACAGCTTTAATAGTTATCGGCTGTGAAATGAGTACACCAAACAATGTCACTATAAATTCTGTGA
ATGCAACTTCAGAAATGCGCGTAGTTAAACATACTATGGGCGAAACAAAAATACCTTTGCGTCCACAGAGGGTAGTTGTA
CTAGGTGGCTTAGATAATATTTTAGCTTTAGGGGTAAAACCAATAGCAGCTACAACATTCAGTGATGATAACTTTGCTGA
TTACTTACAAGATGTAACATCAGGAATTGAAAAAATTGGTATTAATGGTCAACCAAATCTAGAAAAGATTTTATATCTCA
AACCAGACTTAATTTTAGGTTTTTCTTGGGATGCTGAATTATATGGACAACTATCGCAAATTGCCCCAACAGTATTGGCA
GATCAAGATAGCGATTGGAAAGATTGGTTAAAGAAATATGCGGAAGCAGTTGGAGAAACAGCAAAAGCTGAAAAATTGTT
GCAAGAATATGATCAAAGAATGGAAAGCCTGCGTCAACAAATGGGAGATACCCTTTCCCAAACCAAAGTATCTTTAGTCA
ACTTTTGGGCTAATTTTACTCGTCTTTATATGAATAATTCCTTTGGTGGATCAATCCTCAAAGAAATCGGTTTACCTCGT
CCTAAATATCAAGATAAAGATAAAAATCACGAAAATATCTCTTTAGAATTAATTCCTCAGATCAATGGTGATGTTATTTT
CTTAATTCTTGGAGGACACAATGAATCAAGGTTGAAACAATTTACCAATCATCCTTTGTGGTCGCAGTTACAAGCTGTAC
AAAAAAATCAGGTTTATCCAGTTACAGGTGATGTTTGGATTTCTGCATGGGGAATCATTGGAGCTAATCTTGTTTTGGAT
GACTTATTCAAATATTTGCTTGAGTAA

Upstream 100 bases:

>100_bases
ATGCGGCAATACTGAATTAGTAAAGATTTCTCAATTTAGCATTACAGAACTTGTTGATATAGCCACATCATTAATCGCAG
TTTAATTAATCATCAGGAAA

Downstream 100 bases:

>100_bases
TTACAGCGCTTAATCTATATAAAATGTTTCTTCACTGCGCTGAGGACTCTGCACCCTGCAAAAAGCCGCAGAGGCTTCTA
CAAAATGACAATTCTATAGC

Product: iron(III) dicitrate-binding periplasmic protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSIKNNLFIRVFTFLHHILNYHQIKLILFSIITALIVIGCEMSTPNNVTINSVNATSEMRVVKHTMGETKIPLRPQRVVV
LGGLDNILALGVKPIAATTFSDDNFADYLQDVTSGIEKIGINGQPNLEKILYLKPDLILGFSWDAELYGQLSQIAPTVLA
DQDSDWKDWLKKYAEAVGETAKAEKLLQEYDQRMESLRQQMGDTLSQTKVSLVNFWANFTRLYMNNSFGGSILKEIGLPR
PKYQDKDKNHENISLELIPQINGDVIFLILGGHNESRLKQFTNHPLWSQLQAVQKNQVYPVTGDVWISAWGIIGANLVLD
DLFKYLLE

Sequences:

>Translated_328_residues
MSIKNNLFIRVFTFLHHILNYHQIKLILFSIITALIVIGCEMSTPNNVTINSVNATSEMRVVKHTMGETKIPLRPQRVVV
LGGLDNILALGVKPIAATTFSDDNFADYLQDVTSGIEKIGINGQPNLEKILYLKPDLILGFSWDAELYGQLSQIAPTVLA
DQDSDWKDWLKKYAEAVGETAKAEKLLQEYDQRMESLRQQMGDTLSQTKVSLVNFWANFTRLYMNNSFGGSILKEIGLPR
PKYQDKDKNHENISLELIPQINGDVIFLILGGHNESRLKQFTNHPLWSQLQAVQKNQVYPVTGDVWISAWGIIGANLVLD
DLFKYLLE
>Mature_327_residues
SIKNNLFIRVFTFLHHILNYHQIKLILFSIITALIVIGCEMSTPNNVTINSVNATSEMRVVKHTMGETKIPLRPQRVVVL
GGLDNILALGVKPIAATTFSDDNFADYLQDVTSGIEKIGINGQPNLEKILYLKPDLILGFSWDAELYGQLSQIAPTVLAD
QDSDWKDWLKKYAEAVGETAKAEKLLQEYDQRMESLRQQMGDTLSQTKVSLVNFWANFTRLYMNNSFGGSILKEIGLPRP
KYQDKDKNHENISLELIPQINGDVIFLILGGHNESRLKQFTNHPLWSQLQAVQKNQVYPVTGDVWISAWGIIGANLVLDD
LFKYLLE

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=261, Percent_Identity=30.6513409961686, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786807, Length=277, Percent_Identity=25.6317689530686, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 37091; Mature: 36960

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKNNLFIRVFTFLHHILNYHQIKLILFSIITALIVIGCEMSTPNNVTINSVNATSEMR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHH
VVKHTMGETKIPLRPQRVVVLGGLDNILALGVKPIAATTFSDDNFADYLQDVTSGIEKIG
HHHHHCCCCCCCCCCCEEEEECCCHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHCC
INGQPNLEKILYLKPDLILGFSWDAELYGQLSQIAPTVLADQDSDWKDWLKKYAEAVGET
CCCCCCHHEEEEECCCEEEEECCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCH
AKAEKLLQEYDQRMESLRQQMGDTLSQTKVSLVNFWANFTRLYMNNSFGGSILKEIGLPR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
PKYQDKDKNHENISLELIPQINGDVIFLILGGHNESRLKQFTNHPLWSQLQAVQKNQVYP
CCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
VTGDVWISAWGIIGANLVLDDLFKYLLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIKNNLFIRVFTFLHHILNYHQIKLILFSIITALIVIGCEMSTPNNVTINSVNATSEMR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHH
VVKHTMGETKIPLRPQRVVVLGGLDNILALGVKPIAATTFSDDNFADYLQDVTSGIEKIG
HHHHHCCCCCCCCCCCEEEEECCCHHHHHHCCCHHEEEECCCCCHHHHHHHHHHHHHHCC
INGQPNLEKILYLKPDLILGFSWDAELYGQLSQIAPTVLADQDSDWKDWLKKYAEAVGET
CCCCCCHHEEEEECCCEEEEECCCHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHCCH
AKAEKLLQEYDQRMESLRQQMGDTLSQTKVSLVNFWANFTRLYMNNSFGGSILKEIGLPR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
PKYQDKDKNHENISLELIPQINGDVIFLILGGHNESRLKQFTNHPLWSQLQAVQKNQVYP
CCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCC
VTGDVWISAWGIIGANLVLDDLFKYLLE
CCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]