Click here to switch to the map view.

The map label for this gene is aksA

Identifier: 17228902

GI number: 17228902

Start: 1671878

End: 1673011

Strand: Direct

Name: aksA

Synonym: alr1407

Alternate gene names: 17228902

Gene position: 1671878-1673011 (Clockwise)

Preceding gene: 17228901

Following gene: 17228903

Centisome position: 26.07

GC content: 46.91

Gene sequence:

>1134_bases
ATGAATAAAGTTCTCATTAATGACACTACATTACGTGATGGCGAACAAGCCGCAGGTGTGGTTTTTACCTTAGAAGAGAA
AGTGGCGATCGCTAAATTTCTCGATACCATCGGCGTACCCGAATTAGAAGTGGGGATTCCGGCGATGGGTGAGGAAGAAA
TGCGGGCGATCTGCGCCATTTCTAACTTAGGTTTAAAAGCTAACCTTCTGGCGTGGAACCGCGCTGTGATCTCAGATATC
AAAGCTTCTGTTGCTTGCGGGATGGAGCGAGTGCATATTGCGATCCCCGTTTCGGGGATTCAAATCGCCGCAAAATTCCA
CGGACAATGGCGGGTAAGTCTGCAAAGACTCAAAGATTGTATCAGCTTCGCAGTTGATCAAGGTCTTTGGGTAGCAGTAG
GTGGGGAAGATTCCTCTAGGGCTGATGAAAACTTCCTCTTGGATGTAGCATTATACGCCCAAGAATGGGGTGCATCCCGA
TTTCGTTTCTGTGACACAGTAGGAGTCCTTGATCCTTTCACCACTTACGGGAAAGTTAAGCTACTTGTCTCAGCTTTAAC
AATTCCTGTAGAAGTCCATACCCACAATGATTTTGGTATGGCGACAGCTAACGCCCTAGCAGGAATTAAAGCTGGAGCCT
CATCTGTCAATACTACCGTGATTGGGTTGGGTGAAAGAGCAGGTAACGCCGCCTTAGAAGAAGTCGTCATGGCTATCAAA
CGCATCTATGGCGTTGATATGGGGATTGACACACCCCGTTTGTTGGAGTTGTCCCAACTGGTAGCAGCAGCCTCCGGTGC
TAATGTCCCACCTTGGAAGGCGATCGTTGGTGAAAATACCTTTGCTCACGAATCAGGTATTCATGCACATGGTGTACTGC
AAAATCCTGATACCTATGAACCATTCGCACCTGAAGAGGTCGGTTGGGAACGCCGCTTAGTTGTAGGTAAACATTCTGGC
AGACATTCAGTCTCCAACTTGCTAGAACAGCATGGTATTTTTTTGAACCCAGAAGAAACCCAGTCTGTTTTAGATGCAGT
GCGCCAACAGTCAATTAAGAAAAAACGCAGTTTGACTACAGAAGAACTGTTGAACTTGGTCAAAGAACAGAGGTATTCTC
ATGCAGCGCGATGA

Upstream 100 bases:

>100_bases
CAAGGTGAAAAACAGGGATTTATCACTACTAGTGATTGCAGAAAAACAAACTCTCAACCTAAAAAGAATATTAAGAAAAT
TCCTAAGAGGGACGCTTGAG

Downstream 100 bases:

>100_bases
AAACGAGTTAGAGTCAGAACCCGTTTATGAGATTGGCGAAAAAGTCCGGCTTCGTAAACAAATTAAAAATGATGGCACAT
TTCCAGGCAGAGACCTTGGG

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAISNLGLKANLLAWNRAVISDI
KASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQRLKDCISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASR
FRFCDTVGVLDPFTTYGKVKLLVSALTIPVEVHTHNDFGMATANALAGIKAGASSVNTTVIGLGERAGNAALEEVVMAIK
RIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHAHGVLQNPDTYEPFAPEEVGWERRLVVGKHSG
RHSVSNLLEQHGIFLNPEETQSVLDAVRQQSIKKKRSLTTEELLNLVKEQRYSHAAR

Sequences:

>Translated_377_residues
MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAISNLGLKANLLAWNRAVISDI
KASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQRLKDCISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASR
FRFCDTVGVLDPFTTYGKVKLLVSALTIPVEVHTHNDFGMATANALAGIKAGASSVNTTVIGLGERAGNAALEEVVMAIK
RIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHAHGVLQNPDTYEPFAPEEVGWERRLVVGKHSG
RHSVSNLLEQHGIFLNPEETQSVLDAVRQQSIKKKRSLTTEELLNLVKEQRYSHAAR
>Mature_377_residues
MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAISNLGLKANLLAWNRAVISDI
KASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQRLKDCISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASR
FRFCDTVGVLDPFTTYGKVKLLVSALTIPVEVHTHNDFGMATANALAGIKAGASSVNTTVIGLGERAGNAALEEVVMAIK
RIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHAHGVLQNPDTYEPFAPEEVGWERRLVVGKHSG
RHSVSNLLEQHGIFLNPEETQSVLDAVRQQSIKKKRSLTTEELLNLVKEQRYSHAAR

Specific function: This protein is a Fe-Mo-cofactor biosynthetic component

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family

Homologues:

Organism=Escherichia coli, GI1786261, Length=367, Percent_Identity=34.3324250681199, Blast_Score=172, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6320019, Length=372, Percent_Identity=29.8387096774194, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=29.5081967213115, Blast_Score=167, Evalue=2e-42,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NIFV1_NOSS1 (Q44290)

Other databases:

- EMBL:   X99902
- EMBL:   BA000019
- PIR:   AD1982
- RefSeq:   NP_485450.1
- ProteinModelPortal:   Q44290
- SMR:   Q44290
- STRING:   Q44290
- GeneID:   1105002
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1407
- NMPDR:   fig|103690.1.peg.1717
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   ERQISAK
- PhylomeDB:   Q44290
- ProtClustDB:   PRK11858
- BioCyc:   NSP103690:ALR1407-MONOMER
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02660

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.14

Molecular weight: Translated: 40851; Mature: 40851

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAI
CCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
SNLGLKANLLAWNRAVISDIKASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQRLKDC
HCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCHHHHHHHHHHH
ISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASRFRFCDTVGVLDPFTTYGKVK
HHHHHCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHH
LLVSALTIPVEVHTHNDFGMATANALAGIKAGASSVNTTVIGLGERAGNAALEEVVMAIK
HHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
RIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHAHGVLQNPDTYE
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEECCCCCCC
PFAPEEVGWERRLVVGKHSGRHSVSNLLEQHGIFLNPEETQSVLDAVRQQSIKKKRSLTT
CCCCHHCCCCEEEEEECCCCHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCH
EELLNLVKEQRYSHAAR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKVLINDTTLRDGEQAAGVVFTLEEKVAIAKFLDTIGVPELEVGIPAMGEEEMRAICAI
CCCEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHH
SNLGLKANLLAWNRAVISDIKASVACGMERVHIAIPVSGIQIAAKFHGQWRVSLQRLKDC
HCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCEEEEEEECCCHHHHHHHHHHH
ISFAVDQGLWVAVGGEDSSRADENFLLDVALYAQEWGASRFRFCDTVGVLDPFTTYGKVK
HHHHHCCCEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHH
LLVSALTIPVEVHTHNDFGMATANALAGIKAGASSVNTTVIGLGERAGNAALEEVVMAIK
HHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
RIYGVDMGIDTPRLLELSQLVAAASGANVPPWKAIVGENTFAHESGIHAHGVLQNPDTYE
HHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEECCCCCCC
PFAPEEVGWERRLVVGKHSGRHSVSNLLEQHGIFLNPEETQSVLDAVRQQSIKKKRSLTT
CCCCHHCCCCEEEEEECCCCHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCH
EELLNLVKEQRYSHAAR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9139910; 11759840