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The map label for this gene is leuB [H]

Identifier: 17228808

GI number: 17228808

Start: 1557607

End: 1558695

Strand: Direct

Name: leuB [H]

Synonym: alr1313

Alternate gene names: 17228808

Gene position: 1557607-1558695 (Clockwise)

Preceding gene: 17228807

Following gene: 17228809

Centisome position: 24.29

GC content: 47.66

Gene sequence:

>1089_bases
ATGACTCAGAACTATCGAATTACCCTACTCCCTGGCGATGGTATTGGCCCTGAAATTATGGCAGTGGCGGTAGATGTGCT
GAAAGTCGTAGGGCAGCAATTTGATATTCAGTTTGATTTCCAAGAAGCTTTGATTGGTGGTGCAGCAATTGACGCGACAG
GCGAACCTCTGCCATCGGCTACTTTAGATACTTGTCGCCACAGTGATGCTGTATTACTCGCTGCTATTGGTGGTTATAAG
TGGGATTCCTTACCCCCTCACCAACGCCCGGAAGGGGGTTTGTTGGGATTGCGTGCGGGATTAGAATTATTTGCCAATTT
ACGCCCAGCCCAAATTTTACCGCAGTTAATCGACGCTTCGACTTTGAAGCGAGAAGTTGTGGAAGGTGTAGATATTATGG
TGGTGCGTGAACTCACTGGTGGGATTTACTTTGGTAAACCCAGAGGAATCTTTACTACTGAGACTGGCGAGAAACGTGGC
GTTAATACGATGGTTTATACAGAATCGGAAATCGACAGAATTGGCCGGATAGCTTTTGAGACTGCGCGGAAACGTCGGGG
TAAACTCTGTTCAGTAGATAAAGCCAATGTATTAGATGTATCCCAATTGTGGCGCGATCGCATCACCAATCTTTCCCAAG
AATACCCAGACGTGGAACTTTCCCATCTATATGTAGATAATGCCGCCATGCAATTGGTACGCGCTCCCAAGCAGTTCGAT
ACCATCGTTACAGGCAACTTGTTTGGGGATATTCTCTCGGATGCGGCTGCCATGCTTACAGGTAGTATTGGAATGTTACC
CTCGGCTAGTCTTGGGGCTTCTGGGCCGGGAGTCTTTGAACCAGTCCACGGTTCTGCCCCAGATATCGCAGGACAGGATA
AAGCCAACCCCCTCGCCCAAGTTTTGAGTGCGGCGATGATGTTGCGTTACGCTTTAGACCAACCACAAGCAGCAGACAAA
ATAGAACAGGCTGTATTACAAGTTTTAGAGCAAGGTGATCGCACAGGGGATATAATTTCAGTGGGTATGAATCTTTTGGG
TTGCCGGGGTATGGGTGACTCCCTCATAAAGGCATTAGCAAAATCATAA

Upstream 100 bases:

>100_bases
CTACATTCCCCAATTACAAATTACAAATTACGAATTACGTTGGTACACTTGCTCGGTATGATATTTAATCTGCAAGCATT
TACCAAATGAAAATTCAGCT

Downstream 100 bases:

>100_bases
TTTCCCGGTGAAAACAGGCAAATTGGCAACCATTCCCGGATGTTTCGGCTAAACTGGAACCAAAATCTAGCAATCACATA
ACAGCATAGTGTACGCTTTA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSATLDTCRHSDAVLLAAIGGYK
WDSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDASTLKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRG
VNTMVYTESEIDRIGRIAFETARKRRGKLCSVDKANVLDVSQLWRDRITNLSQEYPDVELSHLYVDNAAMQLVRAPKQFD
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQVLSAAMMLRYALDQPQAADK
IEQAVLQVLEQGDRTGDIISVGMNLLGCRGMGDSLIKALAKS

Sequences:

>Translated_362_residues
MTQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSATLDTCRHSDAVLLAAIGGYK
WDSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDASTLKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRG
VNTMVYTESEIDRIGRIAFETARKRRGKLCSVDKANVLDVSQLWRDRITNLSQEYPDVELSHLYVDNAAMQLVRAPKQFD
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQVLSAAMMLRYALDQPQAADK
IEQAVLQVLEQGDRTGDIISVGMNLLGCRGMGDSLIKALAKS
>Mature_361_residues
TQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSATLDTCRHSDAVLLAAIGGYKW
DSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDASTLKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRGV
NTMVYTESEIDRIGRIAFETARKRRGKLCSVDKANVLDVSQLWRDRITNLSQEYPDVELSHLYVDNAAMQLVRAPKQFDT
IVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQVLSAAMMLRYALDQPQAADKI
EQAVLQVLEQGDRTGDIISVGMNLLGCRGMGDSLIKALAKS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=332, Percent_Identity=34.9397590361446, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI28178821, Length=316, Percent_Identity=31.0126582278481, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=31.0126582278481, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=29.9120234604106, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=29.5522388059701, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=37.3333333333333, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=52.3415977961432, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1788101, Length=348, Percent_Identity=33.6206896551724, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787381, Length=409, Percent_Identity=27.3838630806846, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71986051, Length=350, Percent_Identity=36, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17550882, Length=348, Percent_Identity=30.4597701149425, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=342, Percent_Identity=30.7017543859649, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=340, Percent_Identity=31.1764705882353, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=349, Percent_Identity=44.9856733524355, Blast_Score=277, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=342, Percent_Identity=33.9181286549708, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324709, Length=345, Percent_Identity=33.6231884057971, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6324291, Length=348, Percent_Identity=31.3218390804598, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24643268, Length=346, Percent_Identity=33.5260115606936, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643270, Length=346, Percent_Identity=33.5260115606936, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24661184, Length=366, Percent_Identity=31.4207650273224, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078639, Length=335, Percent_Identity=27.1641791044776, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281362242, Length=352, Percent_Identity=29.5454545454545, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648872, Length=352, Percent_Identity=29.5454545454545, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20130355, Length=352, Percent_Identity=22.4431818181818, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38770; Mature: 38639

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECHHHHHHCCCEECCCCCCCCCH
TLDTCRHSDAVLLAAIGGYKWDSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDAS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHH
TLKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRGVNTMVYTESEIDRIGRIAFE
HHHHHHHCCCCEEEEEEHHCCEEECCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHH
TARKRRGKLCSVDKANVLDVSQLWRDRITNLSQEYPDVELSHLYVDNAAMQLVRAPKQFD
HHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHH
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQ
HEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSAAMMLRYALDQPQAADKIEQAVLQVLEQGDRTGDIISVGMNLLGCRGMGDSLIKALA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
KS
CC
>Mature Secondary Structure 
TQNYRITLLPGDGIGPEIMAVAVDVLKVVGQQFDIQFDFQEALIGGAAIDATGEPLPSA
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECHHHHHHCCCEECCCCCCCCCH
TLDTCRHSDAVLLAAIGGYKWDSLPPHQRPEGGLLGLRAGLELFANLRPAQILPQLIDAS
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHH
TLKREVVEGVDIMVVRELTGGIYFGKPRGIFTTETGEKRGVNTMVYTESEIDRIGRIAFE
HHHHHHHCCCCEEEEEEHHCCEEECCCCCEEECCCCCCCCCCEEEEEHHHHHHHHHHHHH
TARKRRGKLCSVDKANVLDVSQLWRDRITNLSQEYPDVELSHLYVDNAAMQLVRAPKQFD
HHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCHHHHH
TIVTGNLFGDILSDAAAMLTGSIGMLPSASLGASGPGVFEPVHGSAPDIAGQDKANPLAQ
HEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSAAMMLRYALDQPQAADKIEQAVLQVLEQGDRTGDIISVGMNLLGCRGMGDSLIKALA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHH
KS
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA