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The map label for this gene is miaB [H]

Identifier: 17228807

GI number: 17228807

Start: 1556074

End: 1557438

Strand: Direct

Name: miaB [H]

Synonym: alr1312

Alternate gene names: 17228807

Gene position: 1556074-1557438 (Clockwise)

Preceding gene: 17228806

Following gene: 17228808

Centisome position: 24.26

GC content: 47.03

Gene sequence:

>1365_bases
ATGACCACTTCTAACCGCCGCTATCACATTACTACCTTTGGTTGCCAAATGAATAAAGCCGACTCAGAGCGCATGGCTGG
CATCTTAGAAGACATGGGCTTTAAGTTTTCCGAAGACCCTAATAATGCAGATTTAATTCTCTACAATACCTGCACAATTC
GGGATAATGCTGAACAGAAAGTCTATTCTTACCTTGGTAGACAAGCCAAGCGCAAACACGAACAACCAGACTTAACCTTA
GTTGTGGCTGGTTGTGTTGCTCAACAGGAAGGCGAAGCACTGCTGCGGCGAGTGCCAGAACTAGATTTAGTCATGGGGCC
ACAACACGCCAACCGTCTCAAAGATTTGCTGGAGTCAGTGTTTGCAGGTAATCAGGTCGTCGCCACTGAAGCCGTCCATA
TTATGGAAGATATCACCCAGGCGCGGCGAGATAGCACCGTTACAGCTTGGGTAAATGTGATTTACGGCTGCAATGAACGC
TGTACTTATTGCGTAGTTCCCAACGTGCGCGGCGTGGAACAGTCCCGCACACCGGCAGCCGTTCGTGCGGAAATGGAAGA
ATTAGGACGGCAAGGATACAAAGAAATCACCCTCCTTGGTCAAAATATCGACGCTTACGGTAGGGATTTACCCGGCGCGA
CACCAGAAGGACGACACCTACACACCTTTACAGACTTGCTATATTACGTCCATGATGTGCCAGGCGTAGAAAGAATCAGA
TTTGCTACTAGTCACCCCCGTTATTTTACGGAGAGACTAATTAAAGCTTGTGCAGAGTTACCCAAAGTTTGCGAACACTT
CCACATTCCTTTTCAATCCGGGGATAACCAACTATTAAAAGCAATGGCGCGGGGTTACACTCAGGAGAAATATCGTCGGA
TTATCGACACAATTCGTAGATATATGCCAGATGCTTCGATTAGTGCCGATGCAATTGTCGGTTTCCCAGGAGAGACAGAG
GAACAGTTTGAAAATACGTTGAAACTGGTAGATGATATTGGCTTTGACCAATTAAACACCGCCGCCTATTCACCCCGTCC
CGGCACACCAGCCGCTTTATGGGAAAATCAACTGAGTGAAGAGGTGAAGAGCGATCGCCTACAAAGATTAAACCATTTAG
TGAACGTCAAAGCAGCCGAGCGATCGCAACGTTACATGGGACGTATCGAGGAAGTCTTAGTAGAAGAGCAGAACCCCAAA
GATCAAACCCAAGTCATGGGACGGACAGGCGGTAATCGTCTCACCTTCTTCAAGGGAGATATTCATGAACTAAAAGGGCA
ATTAGTCATGGTGAAAATTAACGAAGTTCGCGCTTTTAGCTTGACTGGTGAACCGATAGAAATGCGGCAAGCTCTGCCAA
TTTAA

Upstream 100 bases:

>100_bases
TCCCAGCTTGGGGGTTGTTAAAGTTGCTCGACAGTCACTAGAGTAAATAGAGAAGCACACAAAGCATATTTAACATAAAG
CTAACTAAGCATAATTGCCC

Downstream 100 bases:

>100_bases
ACAAATTTATACGAATGCCTCATGGCATAATTACAATCGCTCGGTGTAGAACCAGAGGCTTAAATAGCCTACATTCCCCA
ATTACAAATTACAAATTACG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTL
VVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPAAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIR
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETE
EQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEEVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPK
DQTQVMGRTGGNRLTFFKGDIHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI

Sequences:

>Translated_454_residues
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTL
VVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNER
CTYCVVPNVRGVEQSRTPAAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIR
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETE
EQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEEVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPK
DQTQVMGRTGGNRLTFFKGDIHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI
>Mature_453_residues
TTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQKVYSYLGRQAKRKHEQPDLTLV
VAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERC
TYCVVPNVRGVEQSRTPAAVRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIRF
ATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASISADAIVGFPGETEE
QFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEEVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKD
QTQVMGRTGGNRLTFFKGDIHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=479, Percent_Identity=31.9415448851775, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI28872782, Length=479, Percent_Identity=31.9415448851775, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI93277076, Length=451, Percent_Identity=28.8248337028825, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=446, Percent_Identity=39.6860986547085, Blast_Score=323, Evalue=2e-89,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=25.9423503325942, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=33.0434782608696, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI71996771, Length=385, Percent_Identity=26.4935064935065, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21356207, Length=479, Percent_Identity=32.3590814196242, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19922432, Length=460, Percent_Identity=29.1304347826087, Blast_Score=165, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51510; Mature: 51379

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQK
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPAA
HCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHH
VRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRR
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
YMPDASISADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
EVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTGGNRLTFFKGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECC
IHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI
HHHHCCCEEEEEEECEEEEEECCCCHHHHHCCCC
>Mature Secondary Structure 
TTSNRRYHITTFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQK
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCHHHH
VYSYLGRQAKRKHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESV
HHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
FAGNQVVATEAVHIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPAA
HCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHH
VRAEMEELGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIR
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEE
FATSHPRYFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRR
ECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
YMPDASISADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSE
HCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHH
EVKSDRLQRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTGGNRLTFFKGD
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEECC
IHELKGQLVMVKINEVRAFSLTGEPIEMRQALPI
HHHHCCCEEEEEEECEEEEEECCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA