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The map label for this gene is lipA2

Identifier: 17228777

GI number: 17228777

Start: 1525389

End: 1526291

Strand: Direct

Name: lipA2

Synonym: alr1282

Alternate gene names: 17228777

Gene position: 1525389-1526291 (Clockwise)

Preceding gene: 17228773

Following gene: 17228778

Centisome position: 23.78

GC content: 48.5

Gene sequence:

>903_bases
ATGACTGCCTCACAATCTGCTCAGTTTAAGTCGGAAATTACGGCAATGCCCAGTTGGTTGCGCCGTCCCATTGGCAAAGC
TAGCGAACTGTCAACAGTACAGCGCATTATTAAGCAGCGTCAAATTCATACAATTTGTGAAGAAGGTCGCTGCCCGAATC
GGGGTGAGTGCTATGCCCAAAAAACGGCTACATTCTTACTTATGGGGCCTACCTGCACCCGTGTCTGTGCTTTTTGTCAA
GTCGATAAAGGTCATGCACCGATGCCACTTGACCCAGAGGAAGGGCAAAAGGTGGCAGAAGCGGTACAACTTTTGGGACT
GCGTTATGTAGTACTTACGTCTGTAGCTCGTGATGATTTACAAGACCAGGGAGCAAGCCACTTTGTCCAAACTATGGAGG
CAATCCGTCAATTAAATCCAGGTACTCAAATTGAAGTTTTGACACCGGATTTTTGGGGTGGTGCAGGTGCGGGGGAATCC
GGACAACGCCAGCGCATAAAAATGATTGTAGAGGCACAACCGGCTTGTTTTAACCATAACATTGAGACAGTGCGCCGCTT
AACCGGGCCAGTACGCCGGGGTGCGAAATACGATCGCTCTTTGCGAGTTCTTTCTCTGGTCAAAGAACTCAACCCCCGTA
TTCCCACCAAATCAGGCTTAATGGTGGGATATGGCGAGACGGTTGCTGAACTAGTTGAAGCAATGACAGACTTAAGGAAC
ATAGGATGCGATCGCCTAACTATTGGTCAGTATATGCGCCCATCCCTAGAACATCTACCAGTCCAAAAATATTGGACACC
AGAAGAATTTGAGCATCTAGGCAAACTAGCCAGAGAAATGGGCTTTAGCCATGTCCGTTCGGCTCCACTAGTTCGCAGTT
CCTATCATGCTGGAGAGGAGTGA

Upstream 100 bases:

>100_bases
CGTCTGTCTTTCATTTGAAGTTATATTTCTCTTCATCCCCCTTCTCCTGCAACCATTACATACAAGAGATATTTATTTTT
ATCTAGTCAACTACAAGCCT

Downstream 100 bases:

>100_bases
AGAGGTAGGGGTGTGGCAATACGGTTCGGTTAAGGGATTTCTTGGTTAAGGCAGGCAGGGGAAGGCAGGGGAAGTCAGGG
GAGAAGAGAAGCAGGGGAAG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MTASQSAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRVCAFCQ
VDKGHAPMPLDPEEGQKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGES
GQRQRIKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPRIPTKSGLMVGYGETVAELVEAMTDLRN
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREMGFSHVRSAPLVRSSYHAGEE

Sequences:

>Translated_300_residues
MTASQSAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRVCAFCQ
VDKGHAPMPLDPEEGQKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGES
GQRQRIKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPRIPTKSGLMVGYGETVAELVEAMTDLRN
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREMGFSHVRSAPLVRSSYHAGEE
>Mature_299_residues
TASQSAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQKTATFLLMGPTCTRVCAFCQV
DKGHAPMPLDPEEGQKVAEAVQLLGLRYVVLTSVARDDLQDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGESG
QRQRIKMIVEAQPACFNHNIETVRRLTGPVRRGAKYDRSLRVLSLVKELNPRIPTKSGLMVGYGETVAELVEAMTDLRNI
GCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREMGFSHVRSAPLVRSSYHAGEE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=296, Percent_Identity=42.2297297297297, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI37577164, Length=259, Percent_Identity=40.5405405405405, Blast_Score=194, Evalue=6e-50,
Organism=Escherichia coli, GI1786846, Length=282, Percent_Identity=44.3262411347518, Blast_Score=256, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI32564533, Length=293, Percent_Identity=42.320819112628, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=301, Percent_Identity=42.5249169435216, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI221513272, Length=290, Percent_Identity=42.7586206896552, Blast_Score=235, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA2_NOSS1 (Q8YXD1)

Other databases:

- EMBL:   BA000019
- PIR:   AG1966
- RefSeq:   NP_485325.1
- ProteinModelPortal:   Q8YXD1
- STRING:   Q8YXD1
- GeneID:   1104877
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1282
- NMPDR:   fig|103690.1.peg.1592
- eggNOG:   COG0320
- HOGENOM:   HBG284542
- OMA:   KTKIPMG
- PhylomeDB:   Q8YXD1
- ProtClustDB:   PRK12928
- BioCyc:   NSP103690:ALR1282-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33492; Mature: 33361

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASQSAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQ
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KTATFLLMGPTCTRVCAFCQVDKGHAPMPLDPEEGQKVAEAVQLLGLRYVVLTSVARDDL
HHEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGESGQRQRIKMIVEAQPACFNHN
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCC
IETVRRLTGPVRRGAKYDRSLRVLSLVKELNPRIPTKSGLMVGYGETVAELVEAMTDLRN
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREMGFSHVRSAPLVRSSYHAGEE
CCCCCEEHHHHHCCHHHHCCCHHCCCHHHHHHHHHHHHHHCHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure 
TASQSAQFKSEITAMPSWLRRPIGKASELSTVQRIIKQRQIHTICEEGRCPNRGECYAQ
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
KTATFLLMGPTCTRVCAFCQVDKGHAPMPLDPEEGQKVAEAVQLLGLRYVVLTSVARDDL
HHEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDQGASHFVQTMEAIRQLNPGTQIEVLTPDFWGGAGAGESGQRQRIKMIVEAQPACFNHN
HHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHCCC
IETVRRLTGPVRRGAKYDRSLRVLSLVKELNPRIPTKSGLMVGYGETVAELVEAMTDLRN
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
IGCDRLTIGQYMRPSLEHLPVQKYWTPEEFEHLGKLAREMGFSHVRSAPLVRSSYHAGEE
CCCCCEEHHHHHCCHHHHCCCHHCCCHHHHHHHHHHHHHHCHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840