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The map label for this gene is nadB

Identifier: 17228712

GI number: 17228712

Start: 1433852

End: 1435588

Strand: Direct

Name: nadB

Synonym: alr1217

Alternate gene names: 17228712

Gene position: 1433852-1435588 (Clockwise)

Preceding gene: 17228711

Following gene: 17228717

Centisome position: 22.36

GC content: 46.57

Gene sequence:

>1737_bases
GTGATTACCCATTATTCATTACCCATTACCCGTTACCCATTACCCAGATTTCTAACCTTGTCACAAATAGAGTTTCCTAG
CCAATTTGATGTTTTAGTGGTCGGTGCTGGTGCGGCTGGACTGTATACGGCATTGTGTCTGCCAGAATCCTTACGAGTCG
GCTTGATTACCAAAGAAACTGTTGCTTTATCTGCTAGTGATTGGGCGCAGGGTGGTATAGCTGCTGCGATCGCCCCTAAT
GATTCTCCTTCTCTACATATTGAAGATACAATTCAAGCAGGAGCAGGCTTGTGTGATGTTGCAGCCGTAGAATTTCTGGC
TCAACAAGCCCCCAAATGTATCGAATCTTTGGTTAACTTGGGAGTTGCTTTTGACCGACACGGACAAGCTTTAGCATTAA
CTTTAGAAGCTGCCCATTCTCGTAACCGTGTTCTTCACGCCGCCGACACTACAGGTAGGGAAGTCACAACTACTCTCACG
GATCAAGTATTACGTCGTCCCAATATCCAAGTAATTCAGCAAGCTTTGGCTTTGAGTCTTTGGCTGGAACCAGAAACTGG
TCATTGTCAAGGAATCAGCCTGTTTTATCAAGGTGCAATTACATGGGTCAGGGCTGGGGCTGTAGTCCTGGCGACTGGTG
GCGGTGGTCAAGTATTTGCCCAAACCACAAACCCGGCGGTCAGTACTGGCGATGGAGTTGCAATTGCATGGCGGGCTGGG
GCTATTCTCCGGGACTTAGAATTTGTCCAATTTCACCCCACAGCCTTAACTAAACCAGGTGCAGACCGTTTCCTGATTAG
TGAAGCAGTCCGTGGTGAAGGCGCACACCTAGTAGATAATGAAGGGCGGCGATTTGCTTTTGACTATCACCCGGCTGGAG
AATTAGCACCCAGAGACGTTGTGAGTCGGGCTATATTTAGTCATTTACAACGCACTGCTGTCGATCCTGCCACAGCCCAT
GTGTGGTTGGATATGCGCCCCATCCCCGAAGACAAGATTCGTCTCCGGTTTCCTAATATTGTTAAGGTTTGTCAGCGTTG
GGGTATAGATGTTTTCCGCGAACCAATTCCTGTAGCCCCAGCCGCCCATTATTGGATGGGTGGTATTGCCACCAATTTAA
AGAATCAAACTAATATACCTGGATTGTATGCAGTGGGAGAAACCGCTAGTACAGGTGTACATGGTGCTAATCGTTTAGCA
AGTAATTCATTGCTGGAGTGTATTGTCTTTGGCGCGCAACTAGCAAATCTCACGCCAGCAGATTTGATACAGCATACAGA
AACACCAGCCTTACCCACAAGAGAATTTAAGATTGATGCCAGCGAGTGGCAACACCAGCAATCACAATTAGCCATACTGC
GAGAAAAGTTACCGCGTCTAGTTTGGCAAAGCGCTGGTATTTGTCGAGAACAGTCTACTTTATCTGTGGCGATCGCTACT
GTAAAATCTTGGCAACAGGATTTTGCAGCGCTGCCTTTAAGCCAATTTTTGTTATCTCTACAACCAAACGAGCCAGTTAG
CTGTAATTTTTCAGATACCGACCAACAACTACGACTCTGGGCAGAAACAGGCAATTTACTCGATGTTGCCTATTTAATAC
TCAAAAGCGCTGCTTTTAGAATTGAAAGCCGTGGCGGACACTTCCGTTTAGACTATCCCCACTCAAATCCTGATTGGCAA
GTCCACTCTCTAGTACAAAAGCACCAATGGTGGCAATCTCCGATTATGAAATCTTAA

Upstream 100 bases:

>100_bases
ATTTTCCGTCAACAAGACTAACCCACTCTCTATCCCAGGGGGTGGGAGTCTTATTATTAAATCAGGGGATAGGTAATAGG
TAATAGGTGATTGGTGATTG

Downstream 100 bases:

>100_bases
ATAATGCTTAAGTATATTCAACTAAGTGTTTAAGTTCCATATACTACGCGATGTGCAACTTATTTTTGAAACCCCTCTCC
AAACCTCTCCCCGAAGCGGA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MITHYSLPITRYPLPRFLTLSQIEFPSQFDVLVVGAGAAGLYTALCLPESLRVGLITKETVALSASDWAQGGIAAAIAPN
DSPSLHIEDTIQAGAGLCDVAAVEFLAQQAPKCIESLVNLGVAFDRHGQALALTLEAAHSRNRVLHAADTTGREVTTTLT
DQVLRRPNIQVIQQALALSLWLEPETGHCQGISLFYQGAITWVRAGAVVLATGGGGQVFAQTTNPAVSTGDGVAIAWRAG
AILRDLEFVQFHPTALTKPGADRFLISEAVRGEGAHLVDNEGRRFAFDYHPAGELAPRDVVSRAIFSHLQRTAVDPATAH
VWLDMRPIPEDKIRLRFPNIVKVCQRWGIDVFREPIPVAPAAHYWMGGIATNLKNQTNIPGLYAVGETASTGVHGANRLA
SNSLLECIVFGAQLANLTPADLIQHTETPALPTREFKIDASEWQHQQSQLAILREKLPRLVWQSAGICREQSTLSVAIAT
VKSWQQDFAALPLSQFLLSLQPNEPVSCNFSDTDQQLRLWAETGNLLDVAYLILKSAAFRIESRGGHFRLDYPHSNPDWQ
VHSLVQKHQWWQSPIMKS

Sequences:

>Translated_578_residues
MITHYSLPITRYPLPRFLTLSQIEFPSQFDVLVVGAGAAGLYTALCLPESLRVGLITKETVALSASDWAQGGIAAAIAPN
DSPSLHIEDTIQAGAGLCDVAAVEFLAQQAPKCIESLVNLGVAFDRHGQALALTLEAAHSRNRVLHAADTTGREVTTTLT
DQVLRRPNIQVIQQALALSLWLEPETGHCQGISLFYQGAITWVRAGAVVLATGGGGQVFAQTTNPAVSTGDGVAIAWRAG
AILRDLEFVQFHPTALTKPGADRFLISEAVRGEGAHLVDNEGRRFAFDYHPAGELAPRDVVSRAIFSHLQRTAVDPATAH
VWLDMRPIPEDKIRLRFPNIVKVCQRWGIDVFREPIPVAPAAHYWMGGIATNLKNQTNIPGLYAVGETASTGVHGANRLA
SNSLLECIVFGAQLANLTPADLIQHTETPALPTREFKIDASEWQHQQSQLAILREKLPRLVWQSAGICREQSTLSVAIAT
VKSWQQDFAALPLSQFLLSLQPNEPVSCNFSDTDQQLRLWAETGNLLDVAYLILKSAAFRIESRGGHFRLDYPHSNPDWQ
VHSLVQKHQWWQSPIMKS
>Mature_578_residues
MITHYSLPITRYPLPRFLTLSQIEFPSQFDVLVVGAGAAGLYTALCLPESLRVGLITKETVALSASDWAQGGIAAAIAPN
DSPSLHIEDTIQAGAGLCDVAAVEFLAQQAPKCIESLVNLGVAFDRHGQALALTLEAAHSRNRVLHAADTTGREVTTTLT
DQVLRRPNIQVIQQALALSLWLEPETGHCQGISLFYQGAITWVRAGAVVLATGGGGQVFAQTTNPAVSTGDGVAIAWRAG
AILRDLEFVQFHPTALTKPGADRFLISEAVRGEGAHLVDNEGRRFAFDYHPAGELAPRDVVSRAIFSHLQRTAVDPATAH
VWLDMRPIPEDKIRLRFPNIVKVCQRWGIDVFREPIPVAPAAHYWMGGIATNLKNQTNIPGLYAVGETASTGVHGANRLA
SNSLLECIVFGAQLANLTPADLIQHTETPALPTREFKIDASEWQHQQSQLAILREKLPRLVWQSAGICREQSTLSVAIAT
VKSWQQDFAALPLSQFLLSLQPNEPVSCNFSDTDQQLRLWAETGNLLDVAYLILKSAAFRIESRGGHFRLDYPHSNPDWQ
VHSLVQKHQWWQSPIMKS

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=533, Percent_Identity=33.0206378986867, Blast_Score=234, Evalue=2e-61,
Organism=Escherichia coli, GI1788928, Length=552, Percent_Identity=41.6666666666667, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1790597, Length=531, Percent_Identity=34.0866290018832, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1786942, Length=572, Percent_Identity=31.4685314685315, Blast_Score=216, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17550100, Length=572, Percent_Identity=32.1678321678322, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17505833, Length=573, Percent_Identity=32.2862129144852, Blast_Score=225, Evalue=6e-59,
Organism=Caenorhabditis elegans, GI71986328, Length=475, Percent_Identity=29.2631578947368, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6322701, Length=587, Percent_Identity=33.5604770017036, Blast_Score=234, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6322416, Length=578, Percent_Identity=33.7370242214533, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322511, Length=468, Percent_Identity=26.0683760683761, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6320788, Length=469, Percent_Identity=28.1449893390192, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI17137288, Length=544, Percent_Identity=34.1911764705882, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24655642, Length=544, Percent_Identity=34.1911764705882, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24655647, Length=544, Percent_Identity=34.1911764705882, Blast_Score=241, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24663005, Length=560, Percent_Identity=31.4285714285714, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_NOSS1 (Q8YXJ6)

Other databases:

- EMBL:   BA000019
- PIR:   AF1958
- RefSeq:   NP_485260.1
- ProteinModelPortal:   Q8YXJ6
- SMR:   Q8YXJ6
- STRING:   Q8YXJ6
- GeneID:   1104812
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr1217
- NMPDR:   fig|103690.1.peg.1527
- eggNOG:   COG0029
- HOGENOM:   HBG293998
- OMA:   AACTGLH
- PhylomeDB:   Q8YXJ6
- ProtClustDB:   PRK07395
- BioCyc:   NSP103690:ALR1217-MONOMER
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 63174; Mature: 63174

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 252-252 ACT_SITE 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITHYSLPITRYPLPRFLTLSQIEFPSQFDVLVVGAGAAGLYTALCLPESLRVGLITKET
CCEEECCCCCCCCCCCCEEEHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECE
VALSASDWAQGGIAAAIAPNDSPSLHIEDTIQAGAGLCDVAAVEFLAQQAPKCIESLVNL
EEEECCCCCCCCEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVAFDRHGQALALTLEAAHSRNRVLHAADTTGREVTTTLTDQVLRRPNIQVIQQALALSL
CEEECCCCCEEEEEEEHHCCCCCEEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHEE
WLEPETGHCQGISLFYQGAITWVRAGAVVLATGGGGQVFAQTTNPAVSTGDGVAIAWRAG
EECCCCCCCCCCHHHHHHHHHHEECCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECC
AILRDLEFVQFHPTALTKPGADRFLISEAVRGEGAHLVDNEGRRFAFDYHPAGELAPRDV
HHHHHCHHEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHH
VSRAIFSHLQRTAVDPATAHVWLDMRPIPEDKIRLRFPNIVKVCQRWGIDVFREPIPVAP
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHCCHHHHCCCCCCCC
AAHYWMGGIATNLKNQTNIPGLYAVGETASTGVHGANRLASNSLLECIVFGAQLANLTPA
CHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
DLIQHTETPALPTREFKIDASEWQHQQSQLAILREKLPRLVWQSAGICREQSTLSVAIAT
HHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHH
VKSWQQDFAALPLSQFLLSLQPNEPVSCNFSDTDQQLRLWAETGNLLDVAYLILKSAAFR
HHHHHHHHHHCCHHHHHHHCCCCCCEECCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHE
IESRGGHFRLDYPHSNPDWQVHSLVQKHQWWQSPIMKS
EECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MITHYSLPITRYPLPRFLTLSQIEFPSQFDVLVVGAGAAGLYTALCLPESLRVGLITKET
CCEEECCCCCCCCCCCCEEEHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEECE
VALSASDWAQGGIAAAIAPNDSPSLHIEDTIQAGAGLCDVAAVEFLAQQAPKCIESLVNL
EEEECCCCCCCCEEEEECCCCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHC
GVAFDRHGQALALTLEAAHSRNRVLHAADTTGREVTTTLTDQVLRRPNIQVIQQALALSL
CEEECCCCCEEEEEEEHHCCCCCEEEECCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHEE
WLEPETGHCQGISLFYQGAITWVRAGAVVLATGGGGQVFAQTTNPAVSTGDGVAIAWRAG
EECCCCCCCCCCHHHHHHHHHHEECCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECC
AILRDLEFVQFHPTALTKPGADRFLISEAVRGEGAHLVDNEGRRFAFDYHPAGELAPRDV
HHHHHCHHEEECCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCHHHH
VSRAIFSHLQRTAVDPATAHVWLDMRPIPEDKIRLRFPNIVKVCQRWGIDVFREPIPVAP
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEECHHHHHHHHHCCHHHHCCCCCCCC
AAHYWMGGIATNLKNQTNIPGLYAVGETASTGVHGANRLASNSLLECIVFGAQLANLTPA
CHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHH
DLIQHTETPALPTREFKIDASEWQHQQSQLAILREKLPRLVWQSAGICREQSTLSVAIAT
HHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHH
VKSWQQDFAALPLSQFLLSLQPNEPVSCNFSDTDQQLRLWAETGNLLDVAYLILKSAAFR
HHHHHHHHHHCCHHHHHHHCCCCCCEECCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHE
IESRGGHFRLDYPHSNPDWQVHSLVQKHQWWQSPIMKS
EECCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840