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The map label for this gene is gatA [H]

Identifier: 17228513

GI number: 17228513

Start: 1187627

End: 1189021

Strand: Direct

Name: gatA [H]

Synonym: alr1018

Alternate gene names: 17228513

Gene position: 1187627-1189021 (Clockwise)

Preceding gene: 17228512

Following gene: 17228519

Centisome position: 18.52

GC content: 47.81

Gene sequence:

>1395_bases
ATGAATGATGCTGTCTCCACCGCTAAAGCTATACGTGAGGGCAAAGTTACCGCCGTAGAGGTGACGCAAGCTGCTTTAGC
GCGGATAGCAGCACAGGATGTAGAACTGAATTGTTTTACAGCTGTAACTAGGGAAACGGCTTTGGCGGATGCAGCACGTA
TTGATAAGGAGATGGCCGCAGGTAATTCTCCTGGGTTACTGGCTGGTGTGCCTTTTGCTGTGAAAAACTTATTTGATATC
GCTGGTTTGACGACTCTGGCGGGGGCGAAAATCAATGCCGAAAATCCTCCAGCTAGCCGAGATGCAACGGCAGTAGCCAA
GCTGAAACAAGCTGGTGCGGTATTGGTAGGCGCTTTAAATATGGATGAATACGCCTACGGATTTGTTACAGAAAATTCTC
ATTATGGTGCTACCCATAACCCCCAAGATTTACAGCGAGTGGCTGGGGGTTCCTCTGGTGGTTCGGCGGCGGCGGTGGCG
GCTGGGTTGGTTACTTTCAGCTTGGGTTCTGATACTAACGGTTCCATTCGTGTCCCGGCGGCGTTGTGTGGTGTGTTTGG
TTTCAAGCCGACTTATGGGCGTTTGTCCCGTGCTGGGGTAGCTTTATTTTCTAGTAGTTTTGACCACATCGGCCCCTTTG
CCCGTTCGGTTGGGGATATCGCAACAATATTTGATATTCTGCAAGGAGAAGACGATCGCGATCCTATCTGCACTCAACGA
CCTGCAAACCCCACTTTACCCCAACTAAATCAAGATATCTCTCATATCAGAATAGCGATCGCTGACGATTATTTCCAAAA
AGGGGCAACATCGGCAGCATTAGCAGCCGTGCAGGAAGTAGCCAATGCTTTAAACGTAACCAATTACGTTACTTTACCAG
AAGCCCACCGCGCCAGGGCAGCCGCATTTGTAATTACCGCCAGTGAAGGCGCAAATTTACATTTAGATAAATTAAGAACA
CGTCCCCAGGACTTTGACCCAGCAACACGCGATCGCTTTTTGGCAGGGGCATTAATTCCTAGTAATTGGTATATACAAGC
ACAACGCTTTCGGCAATGGTACCGCGATCGCATCCGTGAAGTTTTTCAAAATGTCGATGTAATTCTCGCCCCTACCACAC
CTATTACAGCACCGCTAATAGGTCAACAGACAATGATTTTAGATGGGGAGGAAATTCTAGTGCGTCCCCATTTAGGATTG
TTCACTCAACCCTTATCTTTTATTGGCTTACCAGTTTTATCAGTACCAATTCAGTGTCCAAACGCTTTACCTTTGGGTGT
GCAGTTAATCGCCGCCCCATACAATGAAGCTTTGATTTTACAGGTGGCGGCTGTATTAGAAGCCCAAGGTGTAGTTTCAG
CTGTGAGTCAGGTAAATTTAGGACTTACGCGCTGA

Upstream 100 bases:

>100_bases
ATGGGGTGGTGGCAAATTTTGAGAGAATAAAAGCGATCGCTCAACTTGTTAACTCATTTCCACTACCAGAAGCAATAGAA
CCCGCCCCTGTCTTTGAACC

Downstream 100 bases:

>100_bases
ACTAAAAAACTCTTCGGTGGTTAATAGTCCACAGTCCAAAAACCTTGATTTTGACTATGGACTATGGACTATTGACAAAT
TAATCATTGGCTGAGGAAGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MNDAVSTAKAIREGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDKEMAAGNSPGLLAGVPFAVKNLFDI
AGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGATHNPQDLQRVAGGSSGGSAAAVA
AGLVTFSLGSDTNGSIRVPAALCGVFGFKPTYGRLSRAGVALFSSSFDHIGPFARSVGDIATIFDILQGEDDRDPICTQR
PANPTLPQLNQDISHIRIAIADDYFQKGATSAALAAVQEVANALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RPQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQCPNALPLGVQLIAAPYNEALILQVAAVLEAQGVVSAVSQVNLGLTR

Sequences:

>Translated_464_residues
MNDAVSTAKAIREGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDKEMAAGNSPGLLAGVPFAVKNLFDI
AGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGATHNPQDLQRVAGGSSGGSAAAVA
AGLVTFSLGSDTNGSIRVPAALCGVFGFKPTYGRLSRAGVALFSSSFDHIGPFARSVGDIATIFDILQGEDDRDPICTQR
PANPTLPQLNQDISHIRIAIADDYFQKGATSAALAAVQEVANALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RPQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQCPNALPLGVQLIAAPYNEALILQVAAVLEAQGVVSAVSQVNLGLTR
>Mature_464_residues
MNDAVSTAKAIREGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDKEMAAGNSPGLLAGVPFAVKNLFDI
AGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALNMDEYAYGFVTENSHYGATHNPQDLQRVAGGSSGGSAAAVA
AGLVTFSLGSDTNGSIRVPAALCGVFGFKPTYGRLSRAGVALFSSSFDHIGPFARSVGDIATIFDILQGEDDRDPICTQR
PANPTLPQLNQDISHIRIAIADDYFQKGATSAALAAVQEVANALNVTNYVTLPEAHRARAAAFVITASEGANLHLDKLRT
RPQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIREVFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGL
FTQPLSFIGLPVLSVPIQCPNALPLGVQLIAAPYNEALILQVAAVLEAQGVVSAVSQVNLGLTR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=29.5774647887324, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI195972892, Length=498, Percent_Identity=27.5100401606426, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI166795287, Length=185, Percent_Identity=34.5945945945946, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=29.4964028776978, Blast_Score=151, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17556264, Length=391, Percent_Identity=27.1099744245524, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17537465, Length=487, Percent_Identity=24.2299794661191, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17556276, Length=286, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=286, Percent_Identity=30.7692307692308, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17538252, Length=293, Percent_Identity=26.2798634812287, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=372, Percent_Identity=26.3440860215054, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17538254, Length=196, Percent_Identity=28.0612244897959, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=385, Percent_Identity=31.6883116883117, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=471, Percent_Identity=26.7515923566879, Blast_Score=116, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6320448, Length=227, Percent_Identity=29.0748898678414, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648113, Length=484, Percent_Identity=33.8842975206612, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=27.1255060728745, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=26.9617706237425, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=26.9617706237425, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=26.9617706237425, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=26.9617706237425, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI45550774, Length=490, Percent_Identity=27.7551020408163, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648435, Length=490, Percent_Identity=27.7551020408163, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24648437, Length=490, Percent_Identity=27.7551020408163, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356731, Length=235, Percent_Identity=33.6170212765957, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI161078093, Length=488, Percent_Identity=26.844262295082, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24648441, Length=409, Percent_Identity=27.1393643031785, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648439, Length=409, Percent_Identity=27.1393643031785, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49027; Mature: 49027

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAVSTAKAIREGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDKEMAA
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHC
GNSPGLLAGVPFAVKNLFDIAGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALN
CCCCCEEECCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTENSHYGATHNPQDLQRVAGGSSGGSAAAVAAGLVTFSLGSDTNGSIRVPA
CCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCEECHH
ALCGVFGFKPTYGRLSRAGVALFSSSFDHIGPFARSVGDIATIFDILQGEDDRDPICTQR
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PANPTLPQLNQDISHIRIAIADDYFQKGATSAALAAVQEVANALNVTNYVTLPEAHRARA
CCCCCCHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHCCE
AAFVITASEGANLHLDKLRTRPQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIRE
EEEEEEECCCCCEEHHHHHCCCCCCCCHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHH
VFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGLFTQPLSFIGLPVLSVPIQCP
HHCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHHHCCCHHCCCCCCC
NALPLGVQLIAAPYNEALILQVAAVLEAQGVVSAVSQVNLGLTR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNDAVSTAKAIREGKVTAVEVTQAALARIAAQDVELNCFTAVTRETALADAARIDKEMAA
CCCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHC
GNSPGLLAGVPFAVKNLFDIAGLTTLAGAKINAENPPASRDATAVAKLKQAGAVLVGALN
CCCCCEEECCCHHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHHHHCCEEEEEEEC
MDEYAYGFVTENSHYGATHNPQDLQRVAGGSSGGSAAAVAAGLVTFSLGSDTNGSIRVPA
CCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCCEECHH
ALCGVFGFKPTYGRLSRAGVALFSSSFDHIGPFARSVGDIATIFDILQGEDDRDPICTQR
HHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
PANPTLPQLNQDISHIRIAIADDYFQKGATSAALAAVQEVANALNVTNYVTLPEAHRARA
CCCCCCHHHHCCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHCCE
AAFVITASEGANLHLDKLRTRPQDFDPATRDRFLAGALIPSNWYIQAQRFRQWYRDRIRE
EEEEEEECCCCCEEHHHHHCCCCCCCCHHHHHHHEEEECCCCCEEEHHHHHHHHHHHHHH
VFQNVDVILAPTTPITAPLIGQQTMILDGEEILVRPHLGLFTQPLSFIGLPVLSVPIQCP
HHCCCCEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCHHHCCHHHHCCCHHCCCCCCC
NALPLGVQLIAAPYNEALILQVAAVLEAQGVVSAVSQVNLGLTR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA