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The map label for this gene is rsmF [H]

Identifier: 17228503

GI number: 17228503

Start: 1179311

End: 1180264

Strand: Direct

Name: rsmF [H]

Synonym: alr1008

Alternate gene names: 17228503

Gene position: 1179311-1180264 (Clockwise)

Preceding gene: 17228500

Following gene: 17228505

Centisome position: 18.39

GC content: 36.69

Gene sequence:

>954_bases
ATGGAAAAACCATCGAACTTACTGCTTAAACTCGCAAGACGTTTGTTTGAAAATCCTGATAAACAAGAAAAATTTATCGA
TGCGTTAGTTGCTCCTCAGCCTTTTAATCCCTGTATTCTGTGGTGTCAAAATCAGCCTGATAATTCACCTTTTTCCGTAG
AATCTCCATTAAGTTGGCAACCAAAATTTGTTGACCGTCTGCACCTGGGAGAAAAACCCGGACAGCATACCTTACATCAT
CAAGGATATTTCTATTGTTTAGATTTTTCATCTGTATTTGCTGCGTCTATTTTATTAACAATCCCTCAATCAGTAAGCTT
AGTTTTTGATATGTGCGCTGCTCCTGGTGGTAAAAGCGTTTTTGCTTGGCAAGCTTTACACCCAGAATTAATCATTTGTA
ATGAGGTTATTGGTAAACGTTTAGGAATGTTAATTTCTAATTTAAAGCGTTGTCAGATTAGCCCTTCTATTATATTTAAT
AGAGATTCTAGTATTTTTGCAGAAAATATTCCTTTATCTAGTAATTTAGTCATAGTTGATGCCCCCTGTACTGGACAATC
TCTCTTAGCAAAAGGAGAGAAAGCCCCAGGATGTTTTCATCCTACTGCTATTAACAAGAGTGCAAATCGCCAAAAGAGAA
TCATCGCTAATTCTGCACAAATTGTTGCCCCACAAGGTTATCTGGCTTATATGACTTGTACTTATTCCCCTGAAGAGAAT
GAGCAGGTGTGTGAATGGTTTTTGTCACGATTTCCCCACTTTCAAGCAGTGGAGATTAACCATTTACAAGAGTATCAGTC
TCACTTAACAAATATTCCTTGTTATCGGTTGTTCCCACAAGATAGATTAGGTGCTGGTGCGTTCACAGCACTTTTTAAAA
ATACTGAAGTAGGAGAAGTTAAAGAATTAGCTCCACAGATTTTAGATGATTTAGGTATTATTCAGAAATTCTAA

Upstream 100 bases:

>100_bases
TCAAAAGTTCTCTTCTCTCGCTACTCTTACCATTCCCCATTCCCTAGTATTATGAAGCCAAGCCACTTCTAAGCGTTGTT
CTCCTGTTACCGTTTTACTA

Downstream 100 bases:

>100_bases
ATCTTTGTAGCGCAGTTAAGTCGGAATAATTAATCAATATACCAATGATAAAAGGACACAAACATTGTGTCCCTTGAGGT
TGTATTAGAGGTATTCGCTT

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDNSPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPELIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPHFQAVEINHLQEYQSHLTNIPCYRLFPQDRLGAGAFTALFKNTEVGEVKELAPQILDDLGIIQKF

Sequences:

>Translated_317_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDNSPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPELIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPHFQAVEINHLQEYQSHLTNIPCYRLFPQDRLGAGAFTALFKNTEVGEVKELAPQILDDLGIIQKF
>Mature_317_residues
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDNSPFSVESPLSWQPKFVDRLHLGEKPGQHTLHH
QGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSVFAWQALHPELIICNEVIGKRLGMLISNLKRCQISPSIIFN
RDSSIFAENIPLSSNLVIVDAPCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
EQVCEWFLSRFPHFQAVEINHLQEYQSHLTNIPCYRLFPQDRLGAGAFTALFKNTEVGEVKELAPQILDDLGIIQKF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=225, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI76150623, Length=225, Percent_Identity=26.6666666666667, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI11545785, Length=222, Percent_Identity=27.027027027027, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI87081985, Length=229, Percent_Identity=29.6943231441048, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17536757, Length=174, Percent_Identity=27.0114942528736, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319447, Length=160, Percent_Identity=26.875, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=179, Percent_Identity=25.6983240223464, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI22024126, Length=174, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355201, Length=264, Percent_Identity=25, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920494, Length=158, Percent_Identity=28.4810126582279, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 35561; Mature: 35561

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDNSPFSVESPLSWQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKFVDRLHLGEKPGQHTLHHQGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSV
HHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
FAWQALHPELIICNEVIGKRLGMLISNLKRCQISPSIIFNRDSSIFAENIPLSSNLVIVD
EEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEEE
APCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
CCCCCHHHHHCCCCCCCCCCCHHCCCCCCHHHHHEECCCEEECCCCEEEEEEEECCCCCC
EQVCEWFLSRFPHFQAVEINHLQEYQSHLTNIPCYRLFPQDRLGAGAFTALFKNTEVGEV
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCEEEECCCHHCCCHHHHHHHCCCCCHHH
KELAPQILDDLGIIQKF
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKPSNLLLKLARRLFENPDKQEKFIDALVAPQPFNPCILWCQNQPDNSPFSVESPLSWQ
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCC
PKFVDRLHLGEKPGQHTLHHQGYFYCLDFSSVFAASILLTIPQSVSLVFDMCAAPGGKSV
HHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCE
FAWQALHPELIICNEVIGKRLGMLISNLKRCQISPSIIFNRDSSIFAENIPLSSNLVIVD
EEEECCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEECCCCCCCCEEEEE
APCTGQSLLAKGEKAPGCFHPTAINKSANRQKRIIANSAQIVAPQGYLAYMTCTYSPEEN
CCCCCHHHHHCCCCCCCCCCCHHCCCCCCHHHHHEECCCEEECCCCEEEEEEEECCCCCC
EQVCEWFLSRFPHFQAVEINHLQEYQSHLTNIPCYRLFPQDRLGAGAFTALFKNTEVGEV
HHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCEEEECCCHHCCCHHHHHHHCCCCCHHH
KELAPQILDDLGIIQKF
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA