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The map label for this gene is nifH2

Identifier: 17228369

GI number: 17228369

Start: 1008496

End: 1009389

Strand: Direct

Name: nifH2

Synonym: alr0874

Alternate gene names: 17228369

Gene position: 1008496-1009389 (Clockwise)

Preceding gene: 17228366

Following gene: 17228372

Centisome position: 15.72

GC content: 44.52

Gene sequence:

>894_bases
ATGAGTATCGACAAGAAAATTAGACAAATTGCTTTCTACGGTAAAGGCGGTATTGGTAAGTCTACCACTTCTCAAAACAC
CTTGGCAGCTATGGCAGAAATGGGTCAACGCATCCTTATCGTAGGTTGCGACCCTAAAGCTGACTCCACCCGTTTGATGC
TGCACTCCAAAGCTCAAACCACCGTTCTTCACTTGGCTGCTGAACGTGGTGCAGTAGAAGACCTCGAACTCGAAGAAGTA
ATGTTGACCGGCTTCCGTGGCGTTAAGTGCGTGGAATCTGGTGGTCCAGAACCCGGTGTAGGTTGCGCTGGTCGTGGTAT
TATCACCGCCATCAACTTCTTAGAAGAAAACGGCGCTTACCAAGACGTTGATTTCGTATCTTATGACGTATTAGGCGACG
TTGTATGCGGCGGTTTCGCAATGCCTATCCGCGAAAATAAAGCGCAAGAAATATACATCGTCACATCAGGTGAAATGATG
GCAATGTATGCTGCTAACAACATTGCTCGTGGTATTTTGAAATATGCCCACACTGGTGGTGTACGTTTAGGCGGTTTAAT
TTGTAACAGCCGTAACGTTGACAGAGAAATCGAACTTATCGAAACTCTGGCAAAACGTTTGAACACCCAAATGATTCACT
ACGTACCCCGCGACAACATTGTTCAACACGCTGAGTTGCGCCGGATGACAGTTAACGAATACGCACCCGATAGCAACCAA
GGTAACGAATACCGGATTTTAGCTAACAAAATCATCAACAACGAAAATCTGAAGATTCCTACCCCAATTGAAATGGAAGA
ATTAGAAGAGTTGCTGATTGAGTTCGGTATTCTCGAAAGCGAAGAAAATGCTGCAAAAATGATTGCTACAACTTCAGAAA
GCAAATCTAAGTAA

Upstream 100 bases:

>100_bases
CTTGCAATCTTTCCTCAATACAAATCCTTATCTACGCAACCTCTAACCAATATTTACTCTAGAACATTGCATTTACATTC
ATTGCAGGAAAACACAAATC

Downstream 100 bases:

>100_bases
GTAATGTGATGCAGTTCTGATAGGGTTTATAACCCCGACTTTTAAACAAGTCGGGGTTATACCATGTGAATTGGGAATTG
GTATAACGTCCCACTTGCAC

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MSIDKKIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRILIVGCDPKADSTRLMLHSKAQTTVLHLAAERGAVEDLELEEV
MLTGFRGVKCVESGGPEPGVGCAGRGIITAINFLEENGAYQDVDFVSYDVLGDVVCGGFAMPIRENKAQEIYIVTSGEMM
AMYAANNIARGILKYAHTGGVRLGGLICNSRNVDREIELIETLAKRLNTQMIHYVPRDNIVQHAELRRMTVNEYAPDSNQ
GNEYRILANKIINNENLKIPTPIEMEELEELLIEFGILESEENAAKMIATTSESKSK

Sequences:

>Translated_297_residues
MSIDKKIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRILIVGCDPKADSTRLMLHSKAQTTVLHLAAERGAVEDLELEEV
MLTGFRGVKCVESGGPEPGVGCAGRGIITAINFLEENGAYQDVDFVSYDVLGDVVCGGFAMPIRENKAQEIYIVTSGEMM
AMYAANNIARGILKYAHTGGVRLGGLICNSRNVDREIELIETLAKRLNTQMIHYVPRDNIVQHAELRRMTVNEYAPDSNQ
GNEYRILANKIINNENLKIPTPIEMEELEELLIEFGILESEENAAKMIATTSESKSK
>Mature_296_residues
SIDKKIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRILIVGCDPKADSTRLMLHSKAQTTVLHLAAERGAVEDLELEEVM
LTGFRGVKCVESGGPEPGVGCAGRGIITAINFLEENGAYQDVDFVSYDVLGDVVCGGFAMPIRENKAQEIYIVTSGEMMA
MYAANNIARGILKYAHTGGVRLGGLICNSRNVDREIELIETLAKRLNTQMIHYVPRDNIVQHAELRRMTVNEYAPDSNQG
NEYRILANKIINNENLKIPTPIEMEELEELLIEFGILESEENAAKMIATTSESKSK

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFH2_NOSS1 (O30577)

Other databases:

- EMBL:   AF012326
- EMBL:   BA000019
- PIR:   AG1915
- RefSeq:   NP_484917.1
- ProteinModelPortal:   O30577
- SMR:   O30577
- STRING:   O30577
- GeneID:   1104468
- GenomeReviews:   BA000019_GR
- KEGG:   ana:alr0874
- NMPDR:   fig|103690.1.peg.1184
- eggNOG:   COG1348
- HOGENOM:   HBG488165
- OMA:   YRILANK
- ProtClustDB:   PRK13236
- BioCyc:   NSP103690:ALR0874-MONOMER
- HAMAP:   MF_00533
- InterPro:   IPR000392
- InterPro:   IPR005977
- PIRSF:   PIRSF000363
- PRINTS:   PR00091
- TIGRFAMs:   TIGR01287

Pfam domain/function: PF00142 Fer4_NifH

EC number: =1.18.6.1

Molecular weight: Translated: 32603; Mature: 32472

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00746 NIFH_FRXC_1; PS00692 NIFH_FRXC_2; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDKKIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRILIVGCDPKADSTRLMLHSKAQT
CCHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHH
TVLHLAAERGAVEDLELEEVMLTGFRGVKCVESGGPEPGVGCAGRGIITAINFLEENGAY
EEEEEHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
QDVDFVSYDVLGDVVCGGFAMPIRENKAQEIYIVTSGEMMAMYAANNIARGILKYAHTGG
CCCHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCC
VRLGGLICNSRNVDREIELIETLAKRLNTQMIHYVPRDNIVQHAELRRMTVNEYAPDSNQ
EEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
GNEYRILANKIINNENLKIPTPIEMEELEELLIEFGILESEENAAKMIATTSESKSK
CCEEEEEEEEHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
SIDKKIRQIAFYGKGGIGKSTTSQNTLAAMAEMGQRILIVGCDPKADSTRLMLHSKAQT
CHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCHH
TVLHLAAERGAVEDLELEEVMLTGFRGVKCVESGGPEPGVGCAGRGIITAINFLEENGAY
EEEEEHHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCC
QDVDFVSYDVLGDVVCGGFAMPIRENKAQEIYIVTSGEMMAMYAANNIARGILKYAHTGG
CCCHHHHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHCCC
VRLGGLICNSRNVDREIELIETLAKRLNTQMIHYVPRDNIVQHAELRRMTVNEYAPDSNQ
EEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCC
GNEYRILANKIINNENLKIPTPIEMEELEELLIEFGILESEENAAKMIATTSESKSK
CCEEEEEEEEHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840