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The map label for this gene is rph [H]

Identifier: 16767019

GI number: 16767019

Start: 3926679

End: 3927395

Strand: Reverse

Name: rph [H]

Synonym: STM3734

Alternate gene names: 16767019

Gene position: 3927395-3926679 (Counterclockwise)

Preceding gene: 16767021

Following gene: 16767018

Centisome position: 80.85

GC content: 54.95

Gene sequence:

>717_bases
ATGCGTCCAGCAGGCCGTAGCGCTAATCAGGTGCGTCCCGTAACCCTGACCCGTAACTATACAAAACACGCTGAAGGCTC
GGTACTGGTCGAATTTGGAGATACTAAAGTGTTGTGCACTGCCTCCATTGAAGAGGGCGTTCCACGTTTTCTGAAAGGAC
AAGGCCAGGGGTGGATCACCGCAGAGTATGGCATGTTGCCTCGCGCTACCCATACCCGTAACGCGCGCGAAGCGGCAAAG
GGTAAGCAGGGCGGCCGTACTATGGAAATTCAGCGTTTGATCGCGCGTGCGCTGCGTGCGGCGGTCGATCTGAAAACGCT
CGGCGAATTTACCATTACGCTGGACTGCGATGTAATCCAGGCCGATGGCGGTACGCGTACAGCGTCTATTACCGGTGCCT
GCGTGGCGTTGGCGGATGCGCTGAATAAATTGGTTGCCAACGGTAAGCTGAAAACCAATCCGATGAAAGGCATGGTGGCG
GCGGTTTCCGTAGGCATCGTTAACGGCGAAGCGATCTGCGATCTGGAATATGTTGAAGATTCCGCAGCAGAAACCGACAT
GAACGTCGTGATGACTGAAGACGGCCGTATTATTGAAGTACAGGGTACCGCGGAAGGCGAGCCGTTCAGCCATGAAGAAC
TTCTCACTTTGTTGGCGCTGGCCCGAGGGGGAATTGAATCCATTGTGGCGACGCAGAAGGCGGCGTTAGAAAATTGA

Upstream 100 bases:

>100_bases
ATGAGGGGATTATAGCTATCCAGGCCTTGTCAGGATAGGAATAACCATCGGAAGTCCGTATAATGCGCAGCCACATTCGT
TTTAAGCCGGAGATCTCAAT

Downstream 100 bases:

>100_bases
TTTTAAGGCGACTGAAAAGTCGCCTTTTTTTTGTCCGTAAATTTCGTAAATGATGAAAGTAAGAAAAGGAGCGAATCCAT
GAAACCGTATCAGCGCCAGT

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPAGRSANQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRATHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKTLGEFTITLDCDVIQADGGTRTASITGACVALADALNKLVANGKLKTNPMKGMVA
AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFSHEELLTLLALARGGIESIVATQKAALEN

Sequences:

>Translated_238_residues
MRPAGRSANQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRATHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKTLGEFTITLDCDVIQADGGTRTASITGACVALADALNKLVANGKLKTNPMKGMVA
AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFSHEELLTLLALARGGIESIVATQKAALEN
>Mature_238_residues
MRPAGRSANQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWITAEYGMLPRATHTRNAREAAK
GKQGGRTMEIQRLIARALRAAVDLKTLGEFTITLDCDVIQADGGTRTASITGACVALADALNKLVANGKLKTNPMKGMVA
AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFSHEELLTLLALARGGIESIVATQKAALEN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=223, Percent_Identity=95.5156950672646, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71981632, Length=189, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25269; Mature: 25269

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPAGRSANQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRATHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKTLGEFTITLDCDVIQ
ECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALNKLVANGKLKTNPMKGMVAAVSVGIVNGEAICDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEEEEEHHHCC
SAAETDMNVVMTEDGRIIEVQGTAEGEPFSHEELLTLLALARGGIESIVATQKAALEN
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPAGRSANQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIEEGVPRFLKGQGQGWIT
CCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCEEEEEECHHHCCHHHHCCCCCEEEE
AEYGMLPRATHTRNAREAAKGKQGGRTMEIQRLIARALRAAVDLKTLGEFTITLDCDVIQ
ECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEE
ADGGTRTASITGACVALADALNKLVANGKLKTNPMKGMVAAVSVGIVNGEAICDLEYVED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCHHHEEEEEEEEEECCCEEEEEHHHCC
SAAETDMNVVMTEDGRIIEVQGTAEGEPFSHEELLTLLALARGGIESIVATQKAALEN
CCCCCCCEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA