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The map label for this gene is citA

Identifier: 16764059

GI number: 16764059

Start: 751371

End: 752675

Strand: Reverse

Name: citA

Synonym: STM0689

Alternate gene names: 16764059

Gene position: 752675-751371 (Counterclockwise)

Preceding gene: 16764060

Following gene: 16764054

Centisome position: 15.5

GC content: 54.02

Gene sequence:

>1305_bases
ATGGCACAACACACACCTGCAACATCGCGCGCCGGCACGTTCGGCGCAATACTGCGCGTGACAAGCGGCAATTTCCTTGA
ACAGTTTGATTTTTTCTTGTTCGGATTTTACGCCACCTATATCGCCAGAACGTTTTTTCCGGCGGAAAGCGAATTTGCCT
CATTAATGTTGACCTTTGCCGTCTTTGGCTCCGGTTTTTTAATGCGCCCCGTCGGCGCGATTGTGCTTGGCGCCTATATT
GACAGAATCGGACGCCGTAAAGGGCTGATGGTGACACTGGCGATTATGGGCTGCGGTACGTTGCTCATCGCCCTCGTCCC
CGGCTACCAAACGATCGGCCTGGCAGCGCCTGCGTTGGTGCTGTTGGGCCGGTTATTACAAGGATTTTCTGCGGGCGTTG
AGTTAGGCGGCGTCTCGGTCTATCTGTCCGAAATCGCAACGCCAGGCAATAAAGGGTTTTATACCAGCTGGCAATCCGCC
AGTCAGCAGGTCGCGATCGTTGTTGCCGCGTTGATTGGTTATAGCCTGAATATCACGCTGGGACACGACGCGATATCGGA
GTGGGGCTGGCGAATTCCGTTCTTTATCGGCTGTATGATCATTCCGCTGATTTTTGTTTTACGTCGTTCATTACAAGAAA
CAGAAGCGTTTTTACAACGCAAGCATCGCCCCGACACCAGGGAAATTTTTGCAACTATCGCCAAAAACTGGCGCATTATT
ACAGCCGGAACGCTGCTGGTGGCGATGACCACCACAACGTTTTATTTTATCACCGTTTATACGCCGACCTATGGCAGAAC
CGTGCTTAATCTCAGCGCGCGGGACAGTTTGATCGTCACCATGTTAGTGGGGGTGTCCAATTTTATCTGGTTACCCATTG
GCGGCGCGATTTCCGACCGGATTGGCCGTCGCGCTGTGTTAATGGGCATTACGTTGCTGGCGCTGATCACCACCTGGCCC
GTCATGCAGTGGCTGACCGCCGCGCCCGACTTTACCCGCATGACGCTGGTACTGCTGTGGTTCTCTTTCTTTTTTGGCAT
GTATAACGGCGCAATGGTCGCGGCGTTAACCGAAGTGATGCCAGTCTATGTGCGTACCGTTGGTTTCTCGCTGGCCTTTA
GCCTGGCGACGGCGATTTTTGGCGGCCTGACGCCGGCCATCTCTACCGCGCTGGTAAAGTTAACCGGCGATAAAAGCTCG
CCCGGCTGGTGGCTGATGTGCGCAGCGTTATGTGGACTTGCCGCGACGGCGATGCTGTTTGTACGTCTGAGTCGCGGCTA
TATCGCGGCAGAAAATAAAGCCTGA

Upstream 100 bases:

>100_bases
GGGCAGTTGAGAAGCGACGCGGAAAACATGCGGGGGATACAGGCAACTGACCCGCAACATCTTACCCATAAAACAATAAA
GACAGTGGAGAGCAAACCCT

Downstream 100 bases:

>100_bases
AAAAACCACGGGCGGAAAACATTCGCCCGTGGGTGGCAAGGCAGGGTTTTATTGCACGTTATTATGACTGCGAATTTCCT
GCCAGACCTTATCGCAGTCA

Product: citrate-proton symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MAQHTPATSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIARTFFPAESEFASLMLTFAVFGSGFLMRPVGAIVLGAYI
DRIGRRKGLMVTLAIMGCGTLLIALVPGYQTIGLAAPALVLLGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSA
SQQVAIVVAALIGYSLNITLGHDAISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRKHRPDTREIFATIAKNWRII
TAGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLIVTMLVGVSNFIWLPIGGAISDRIGRRAVLMGITLLALITTWP
VMQWLTAAPDFTRMTLVLLWFSFFFGMYNGAMVAALTEVMPVYVRTVGFSLAFSLATAIFGGLTPAISTALVKLTGDKSS
PGWWLMCAALCGLAATAMLFVRLSRGYIAAENKA

Sequences:

>Translated_434_residues
MAQHTPATSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIARTFFPAESEFASLMLTFAVFGSGFLMRPVGAIVLGAYI
DRIGRRKGLMVTLAIMGCGTLLIALVPGYQTIGLAAPALVLLGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSA
SQQVAIVVAALIGYSLNITLGHDAISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRKHRPDTREIFATIAKNWRII
TAGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLIVTMLVGVSNFIWLPIGGAISDRIGRRAVLMGITLLALITTWP
VMQWLTAAPDFTRMTLVLLWFSFFFGMYNGAMVAALTEVMPVYVRTVGFSLAFSLATAIFGGLTPAISTALVKLTGDKSS
PGWWLMCAALCGLAATAMLFVRLSRGYIAAENKA
>Mature_433_residues
AQHTPATSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIARTFFPAESEFASLMLTFAVFGSGFLMRPVGAIVLGAYID
RIGRRKGLMVTLAIMGCGTLLIALVPGYQTIGLAAPALVLLGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSAS
QQVAIVVAALIGYSLNITLGHDAISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRKHRPDTREIFATIAKNWRIIT
AGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLIVTMLVGVSNFIWLPIGGAISDRIGRRAVLMGITLLALITTWPV
MQWLTAAPDFTRMTLVLLWFSFFFGMYNGAMVAALTEVMPVYVRTVGFSLAFSLATAIFGGLTPAISTALVKLTGDKSSP
GWWLMCAALCGLAATAMLFVRLSRGYIAAENKA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system)

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Homologues:

Organism=Escherichia coli, GI1790550, Length=418, Percent_Identity=31.3397129186603, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI1788942, Length=417, Percent_Identity=33.8129496402878, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788292, Length=410, Percent_Identity=28.2926829268293, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789941, Length=436, Percent_Identity=28.4403669724771, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082231, Length=202, Percent_Identity=31.1881188118812, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CITA_SALTI (P0A2G4)

Other databases:

- EMBL:   AL627267
- EMBL:   AE014613
- RefSeq:   NP_455250.1
- RefSeq:   NP_805936.1
- ProteinModelPortal:   P0A2G4
- GeneID:   1067275
- GeneID:   1247184
- GenomeReviews:   AE014613_GR
- GenomeReviews:   AL513382_GR
- KEGG:   stt:t2186
- KEGG:   sty:STY0727
- HOGENOM:   HBG757988
- OMA:   ILIACVP
- ProtClustDB:   PRK15075
- BioCyc:   SENT209261:T2186-MONOMER
- BioCyc:   SENT220341:STY0727-MONOMER
- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829
- TIGRFAMs:   TIGR00883

Pfam domain/function: PF00083 Sugar_tr; SSF103473 MFS_gen_substrate_transporter

EC number: NA

Molecular weight: Translated: 47189; Mature: 47058

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS; PS00216 SUGAR_TRANSPORT_1; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xd0de374)-; HASH(0xb2d8768)-; HASH(0xd108d7c)-; HASH(0xd290688)-; HASH(0xd11eb0c)-; HASH(0xd3b5c74)-; HASH(0xd4be4ac)-; HASH(0xd10cda8)-; HASH(0xd109118)-; HASH(0xd10c5a4)-; HASH(0xd444bec)-; HASH(0xd4bb694)-;

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTPATSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIARTFFPAESEFASLMLTFA
CCCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFGSGFLMRPVGAIVLGAYIDRIGRRKGLMVTLAIMGCGTLLIALVPGYQTIGLAAPALV
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSASQQVAIVVAALIGYSLNITL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
GHDAISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRKHRPDTREIFATIAKNWRII
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEE
TAGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLIVTMLVGVSNFIWLPIGGAISDR
EECHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCHHHHH
IGRRAVLMGITLLALITTWPVMQWLTAAPDFTRMTLVLLWFSFFFGMYNGAMVAALTEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
PVYVRTVGFSLAFSLATAIFGGLTPAISTALVKLTGDKSSPGWWLMCAALCGLAATAMLF
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
VRLSRGYIAAENKA
HHHHCCEEEECCCH
>Mature Secondary Structure 
AQHTPATSRAGTFGAILRVTSGNFLEQFDFFLFGFYATYIARTFFPAESEFASLMLTFA
CCCCCCCCCCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFGSGFLMRPVGAIVLGAYIDRIGRRKGLMVTLAIMGCGTLLIALVPGYQTIGLAAPALV
HHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LLGRLLQGFSAGVELGGVSVYLSEIATPGNKGFYTSWQSASQQVAIVVAALIGYSLNITL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEE
GHDAISEWGWRIPFFIGCMIIPLIFVLRRSLQETEAFLQRKHRPDTREIFATIAKNWRII
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEE
TAGTLLVAMTTTTFYFITVYTPTYGRTVLNLSARDSLIVTMLVGVSNFIWLPIGGAISDR
EECHHHHHHHHCEEEEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCEEEEECCCHHHHH
IGRRAVLMGITLLALITTWPVMQWLTAAPDFTRMTLVLLWFSFFFGMYNGAMVAALTEVM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
PVYVRTVGFSLAFSLATAIFGGLTPAISTALVKLTGDKSSPGWWLMCAALCGLAATAMLF
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
VRLSRGYIAAENKA
HHHHCCEEEECCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504