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The map label for this gene is ushA [H]

Identifier: 16763874

GI number: 16763874

Start: 553634

End: 555286

Strand: Direct

Name: ushA [H]

Synonym: STM0494

Alternate gene names: 16763874

Gene position: 553634-555286 (Clockwise)

Preceding gene: 16763871

Following gene: 16763879

Centisome position: 11.4

GC content: 51.72

Gene sequence:

>1653_bases
ATGAAATTTTTGAAACGGGGTGTGGCGCTGGCGTTACTGGCGGCGTTCGCGCTGACGACTCAGCCTGCACAGGCTTACGA
AAAAGATAAAACCTATAAAATTACTATCCTGCATACCAACGATCACCACGGTCACTTCTGGCGCAGCGAATATGGCGAAT
ATGGTCTGGCGGCGCAAAAAACGCTGGTGGACAGTATCCGTAAAGAGGTGGCGCAAGAGGGGGGAAGCGTCCTGTTGTTA
TCCGGCGGCGACATTAATACCGGGGTGCCGGAATCCGATCTCCAGGATGCGGAGCCCGATTTCCGCGGGATGAATCTGAT
TGGCTACGACGCTATGGCCGTCGGTAATCATGAATTTGATAATCCGCTCACCGTATTGCGCCAGCAGGAAAAGTGGGCGA
AGTTTCCCTTTCTTTACGCCAATATTTATCAAAAAAGTACCGGCGAGCGTCTGTTTAAGCCGTGGGCTATTTTTACACGC
CAGGATATAAAAATCGCGGTAATCGGCTTAACCACCGATGACACGGCGAAAATAGGCAACCCGGAATATTTCACCGATAT
TGAGTTTCGTAAACCTGCTGAAGAAGCAAAGGTGGTGATTCAGGAACTTAATATGAATGAAAAACCGGACGTGATTATCG
CGACCACGCATATGGGACATTATGACAACGGCGATCACGGTTCGAACGCGCCGGGCGACGTTGAGATGGCGCGTAGCCTG
CCTGCCGGTTCGTTGGCGATGATTGTGGGCGGTCACTCACAAGACCCGGTATGCATGGCGTCGGAAAATAAAAAACAGGT
GAATTACGTACCGGGAACGCCCTGCGCGCCGGATAAGCAAAATGGCATCTGGATCGTGCAGGCGCATGAGTGGGGTAAAT
ATGTGGGCCGTGCGGATTTCGAATTCCGTAACGGCGAGATGAAAATGGTTAACTACCAGCTTATTCCGGTAAATCTCAAG
AAAAAAGTGACCTGGGATAACGGGAAAAGCGAGCGTGTACTTTACACGCCGGAAATCGCAGAAAATCCGCAAATGCTCTC
GTTATTAACGCCGTTCCAGAATAAAGGTAAAGCGCAACTGGAGGTGAAAATTGGTAGCGTGAATGGCCTTCTTGAAGGCG
ATCGCAGTAAGGTCAGATTTGTCCAGACCAATATGGGACGGGTGATTCTGGCTGCGCAGATCGCGCGCACCGGCGCCGAT
TTTGGCGTGATGAGCGGCGGCGGTATTCGCGACTCGATTGAGGCGGGAGATATTACCTATAAAAGCGTGCTCAAGGTACA
GCCGTTCGGCAACATTGTGGTGTATGCCGATATGAGCGGCAAAGAGGTGGTTGATTATCTCACCGCCGTAGCACAGATGA
AACCGGACTCCGGCGCCTATCCACAGCTCGCCAATGTGAGCTTTGTCGCCAAAGAGGGCAAGCTCACCGATCTGAAAATC
AAAGGCGAGCCTGTTGATCCGGCTAAAACCTATCGCATGGCGACGCTGAGTTTCAACGCCACGGGCGGCGATGGTTATCC
GCGCATTGATAACAAACCGGGCTACGTGAATACCGGGTTTATTGACGCGGAAGTGCTGAAAGAGTTTATTCAGCAAAATT
CACCGCTGGATGCGGCGGCGTTTACGCCAAATGGTGAGGTGAGCTGGCTGTAG

Upstream 100 bases:

>100_bases
TGAAATGTGTTTGAAGGAAGTCTCAATTCTGAAAACATATTTGTCTATTATTGCAAGGAAAGGTAATTTCTGCGGTTGAT
ATTGAGTCAGGGAGAGAAAG

Downstream 100 bases:

>100_bases
CGGTACGGATAGCCATTTCTGCCTGATGGCGCGCAACCTTAAGGCCTACGGATTATGTCTGCTGTAGGCCGGATAAGGCG
TTTGCGCCGCTATCCGGCAA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: UDP-sugar hydrolase; UDP-sugar diphosphatase; UDP-sugar pyrophosphatase; 5'-nucleotidase; 5'-NT [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLYANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMASENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQLANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPNGEVSWL

Sequences:

>Translated_550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLYANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMASENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQLANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPNGEVSWL
>Mature_550_residues
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQKTLVDSIRKEVAQEGGSVLLL
SGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFDNPLTVLRQQEKWAKFPFLYANIYQKSTGERLFKPWAIFTR
QDIKIAVIGLTTDDTAKIGNPEYFTDIEFRKPAEEAKVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
PAGSLAMIVGGHSQDPVCMASENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLK
KKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQLEVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGAD
FGVMSGGGIRDSIEAGDITYKSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQLANVSFVAKEGKLTDLKI
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAAFTPNGEVSWL

Specific function: Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=586, Percent_Identity=25.2559726962457, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=87.6138433515483, Blast_Score=1027, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=557, Percent_Identity=22.0825852782765, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922446, Length=583, Percent_Identity=25.557461406518, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24654424, Length=583, Percent_Identity=25.557461406518, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI28573524, Length=556, Percent_Identity=23.9208633093525, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922444, Length=571, Percent_Identity=22.5919439579685, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24641187, Length=544, Percent_Identity=21.6911764705882, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI221329836, Length=573, Percent_Identity=21.2914485165794, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.6.1.45; =3.1.3.5 [H]

Molecular weight: Translated: 60557; Mature: 60557

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHH
TLVDSIRKEVAQEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLTVLRQQEKWAKFPFLYANIYQKSTGERLFKPWAIFTRQDIKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHCCEEEEEECCEEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEAKVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
CCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMASENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKMVNYQLIPVNLKKKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEECEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEE
EVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGADFGVMSGGGIRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCEEE
KSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQLANVSFVAKEGKLTDLKI
EHEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCEEE
FTPNGEVSWL
ECCCCCEEEC
>Mature Secondary Structure
MKFLKRGVALALLAAFALTTQPAQAYEKDKTYKITILHTNDHHGHFWRSEYGEYGLAAQK
CCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEEEEECCCCCCCHHHHCCCCCCHHHH
TLVDSIRKEVAQEGGSVLLLSGGDINTGVPESDLQDAEPDFRGMNLIGYDAMAVGNHEFD
HHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCEEEECCCCCC
NPLTVLRQQEKWAKFPFLYANIYQKSTGERLFKPWAIFTRQDIKIAVIGLTTDDTAKIGN
CHHHHHHHHHHHHCCCEEEEEHHHCCCCCHHHCCEEEEEECCEEEEEEEECCCCCCCCCC
PEYFTDIEFRKPAEEAKVVIQELNMNEKPDVIIATTHMGHYDNGDHGSNAPGDVEMARSL
CCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHCC
PAGSLAMIVGGHSQDPVCMASENKKQVNYVPGTPCAPDKQNGIWIVQAHEWGKYVGRADF
CCCCEEEEEECCCCCCEEEECCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCE
EFRNGEMKMVNYQLIPVNLKKKVTWDNGKSERVLYTPEIAENPQMLSLLTPFQNKGKAQL
EEECCCEEEEEEEEEEEECEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEE
EVKIGSVNGLLEGDRSKVRFVQTNMGRVILAAQIARTGADFGVMSGGGIRDSIEAGDITY
EEEEECCCCEECCCCCEEEEEECCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCCCCCEEE
KSVLKVQPFGNIVVYADMSGKEVVDYLTAVAQMKPDSGAYPQLANVSFVAKEGKLTDLKI
EHEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCEEEEEE
KGEPVDPAKTYRMATLSFNATGGDGYPRIDNKPGYVNTGFIDAEVLKEFIQQNSPLDAAA
ECCCCCHHHHEEEEEEEEECCCCCCCCCCCCCCCEEECCEECHHHHHHHHHCCCCCCEEE
FTPNGEVSWL
ECCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11429465 [H]